Agilent Technologies Patent Grants

Method for amplification of target nucleic acid

Granted: May 23, 2017
Patent Number: 9657339
A method for amplifying a target nucleic acid is disclosed, which includes: (a) fragmenting a nucleic acid sample to create a target fragment comprising a target nucleic acid and two probe-complementary portions; (b) contacting said fragmented nucleic acid sample with a probe comprising two target fragment-complementary portions complementary to the probe-complementary portions of the target fragment; (c) rendering the fragmented nucleic acid sample single-stranded; (d) allowing the…

Light source with controllable linear polarization

Granted: May 16, 2017
Patent Number: 9651426
A light source having first and second wire-grid polarizers and a laser that emits a beam of linearly polarized light that is characterized by a propagation direction is disclosed. The first wire-grid polarization filter is characterized by a first linear polarization pass direction and a first actuator for causing the first linear polarization pass direction to rotate relative to the beam of linearly polarized light. The second wire-grid polarization filter is characterized by a second…

Immobilized transposase complexes for DNA fragmentation and tagging

Granted: May 9, 2017
Patent Number: 9644199
The present invention provides a simple and rapid method for preparing purified transposase complexes that are highly suited for fragmenting DNA. The method includes forming transposase complexes with oligonucleotide adapters in cell lysate, then purifying the complexes from the other substance in the cell lysate. Purification is accomplished using a specific binding pair, in which one member of the pair is bound to an oligonucleotide adapter of the complex and the other member of the…

GC column connection with a planar connection to mating devices

Granted: May 2, 2017
Patent Number: 9638676
A representative embodiment is directed to a fitting for fluidically coupling a GC column to another structure. The fitting comprises: a first end configured to receive a ferrule having a tubular element disposed therein, the tubular element being oriented in a first direction; and a second end fluidically connected to the first end and having an opening to provide a fluid from the tubular element in a second direction that is different from the first direction. The second end comprises…

Chromatograph

Granted: April 25, 2017
Patent Number: D785198

Determination of a fluid loss of a piston pump based on evacuation time variation

Granted: April 25, 2017
Patent Number: 9631618
A device for determining a leakage of fluid in a piston pump, wherein the piston pump comprises a piston arranged in such a manner that it can reciprocate in a piston chamber for delivering fluid, wherein the device comprises a control unit for controlling the piston in such a manner that the piston executes two piston chamber evacuation processes with different evacuation times, in each case for at least partial evacuating of fluid located in the piston chamber, and a determination unit…

Magnifying assembly for an infrared microscope

Granted: April 18, 2017
Patent Number: 9625695
An infrared microscope includes a sample stage configured to support a sample; an objective configured to focus radiation emanated from the sample to an intermediate image plane between an objective and an infrared detector; and a magnifying assembly including a first set of reflective elements provided in a fixed position and a second set of reflective elements.

Energy resolved time-of-flight mass spectrometry

Granted: April 18, 2017
Patent Number: 9627190
A time-of-flight mass spectrometer (TOF-MS) utilizes an ion dispersion device and a position-sensitive ion detector or an energy-sensitive ion detector to enable measurement of time of flight and kinetic energy of ions arriving at the detector. The measurements may be utilized to improve accuracy in calculating ion masses.

Hydrolytically stable ion-exchange stationary phases and uses thereof

Granted: April 4, 2017
Patent Number: 9610576
The invention provides novel ion-exchange media and related methods for their preparation and use. Ion-exchange stationary phases according to the invention are suitable for chromatographic separation of a variety of biomolecules. Distinguishing characteristics of ion-exchange media according to this invention includes, for example, their ability to separate variants of monoclonal antibodies via cation-exchange liquid chromatography using porous substrates with particle sizes <5 ?m.…

Vacuum scroll pump having pressure-balanced orbiting plate scroll

Granted: March 28, 2017
Patent Number: 9605674
A vacuum scroll pump has a frame, a stationary plate scroll fixed to the frame, an orbiting plate scroll, an eccentric drive mechanism for driving the orbiting plate scroll, and counterbalancing features by which axial loads produced on the eccentric drive mechanism are offset. Scroll blades of the stationary and orbiting plate scrolls are nested to define pockets which constitute a compression stage between opposing front sides of plates of the stationary and orbiting plate scrolls. The…

Fast hybridization for next generation sequencing target enrichment

Granted: March 7, 2017
Patent Number: 9587268
The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.

Plasma cleaning for mass spectrometers

Granted: March 7, 2017
Patent Number: 9589775
A mass spectrometry (MS) system may be cleaned by generating plasma and contacting an internal surface of the system to be cleaned with the plasma. The system may be switched between operating in an analytical mode and in a cleaning mode. In the analytical mode a sample is analyzed, and plasma may or may not be actively generated. In the cleaning mode the plasma is actively generated, and the sample may or may not be analyzed.

Alternating current (AC) coupler for wideband AC signals and related methods

Granted: March 7, 2017
Patent Number: 9590583
An AC coupler for transmitting high-frequency components of a wideband signal includes a signal conductor and a shielding structure arranged as a transmission line. The signal conductor includes a conductive element and a capacitor configured to block direct current (DC) components of the wideband signal while transmitting high-frequency alternating current (AC) components of the wideband signal. The shielding structure is configured for conducting at least the AC components of the…

Synthesis of long fish probes

Granted: February 28, 2017
Patent Number: 9580746
A method comprising: synthesizing a set of overlapping oligonucleotides that comprises probe sequences that hybridize to unique sequences in a chromosome, assembling the overlapping oligonucleotides in a way that produces one or more double stranded polynucleotides that each comprises multiple probe sequences, labeling the one or more double stranded polynucleotides to produce one or more labeled probes, and hybridizing the labeled probes to an intact chromosome, in situ, is provided.

Isolation of RNA-protein complexes using cross-linking reagents and oligonucleotides

Granted: February 21, 2017
Patent Number: 9574236
Provided herein is a method of sample analysis. In certain embodiments, the method comprises: a) cross-linking protein of a cell using a first compound to produce a first cross-linked product comprising cross-linked protein, and RNA; b) contacting the first cross-linked product and a second compound under conditions by which an oligonucleotide portion of the second compound hybridizes to the RNA; c) activating a reaction the first and second compound, thereby covalently crosslinking the…

Data processing for multiplexed spectrometry

Granted: February 21, 2017
Patent Number: 9576778
Multiplexed spectrometry, such as multiplexed ion mobility spectrometry (IMS), time-of-flight mass spectrometry (TOFMS), or hybrid IM-TOFMS, is carried out on a sample, and the resulting measurement data are deconvoluted. Noise may be removed from the measurement data prior to deconvolution. Alternatively or additionally, noise may be removed from the deconvoluted data.

Polymerase idling method for single molecule DNA sequencing

Granted: January 17, 2017
Patent Number: 9546398
A method for sequencing a nucleic acid is provided. In certain embodiments the method comprises obtaining a duplex comprising a nucleic acid and a primer, wherein the primer has a nuclease resistant 3? end, combining the duplex with a chain terminator nucleotide and a proof-reading polymerase to produce a reaction in which the polymerase idles on the added chain terminator nucleotide, identifying the chain terminator nucleotide added to the end of the primer; and adding a…

Mid-infrared scanning system that differentiates between specular and diffuse scattering

Granted: January 17, 2017
Patent Number: 9546905
A method and apparatus for measuring diffuse and specularly reflected light from a sample to provide a reflection spectrum as a function of wavelength and as a function of position on a sample is disclosed. The apparatus includes a MIR light source that generates an illumination beam of linearly polarized light. An illumination system illuminates a location on a specimen with part of the illumination beam. A linear polarization filter characterized by a polarization axis that defines a…

Sample droplet generation from segmented fluid flow and related devices and methods

Granted: January 10, 2017
Patent Number: 9543137
A sample droplet generator transforms a segmented array of sample material into a continuous stream of droplets containing analytes. The droplets may serve as a sample source for a wide range of detectors and analytical instruments. As one example, the droplets may be introduced into an ion source of a spectrometer that measures ions produced from the droplets or photons emitted from the droplets.

Thin field terminator for linear quadrupole ion guides, and related systems and methods

Granted: January 3, 2017
Patent Number: 9536723
A field terminator includes a plurality of electrode plates positioned around a guide axis at a radial distance therefrom. The plates generate a quadrupole DC field such that a polarity on each plate is opposite to a polarity on the plates adjacent thereto. The plates may be positioned at an axial end of a quadrupole ion guide such as a mass filter. In addition to an RF field, the ion guide may generate a quadrupole DC field. The DC field of the plates may be opposite in polarity to that…