Agilent Technologies Patent Grants

Fast hybridization for next generation sequencing target enrichment

Granted: March 7, 2017
Patent Number: 9587268
The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.

Plasma cleaning for mass spectrometers

Granted: March 7, 2017
Patent Number: 9589775
A mass spectrometry (MS) system may be cleaned by generating plasma and contacting an internal surface of the system to be cleaned with the plasma. The system may be switched between operating in an analytical mode and in a cleaning mode. In the analytical mode a sample is analyzed, and plasma may or may not be actively generated. In the cleaning mode the plasma is actively generated, and the sample may or may not be analyzed.

Alternating current (AC) coupler for wideband AC signals and related methods

Granted: March 7, 2017
Patent Number: 9590583
An AC coupler for transmitting high-frequency components of a wideband signal includes a signal conductor and a shielding structure arranged as a transmission line. The signal conductor includes a conductive element and a capacitor configured to block direct current (DC) components of the wideband signal while transmitting high-frequency alternating current (AC) components of the wideband signal. The shielding structure is configured for conducting at least the AC components of the…

Synthesis of long fish probes

Granted: February 28, 2017
Patent Number: 9580746
A method comprising: synthesizing a set of overlapping oligonucleotides that comprises probe sequences that hybridize to unique sequences in a chromosome, assembling the overlapping oligonucleotides in a way that produces one or more double stranded polynucleotides that each comprises multiple probe sequences, labeling the one or more double stranded polynucleotides to produce one or more labeled probes, and hybridizing the labeled probes to an intact chromosome, in situ, is provided.

Isolation of RNA-protein complexes using cross-linking reagents and oligonucleotides

Granted: February 21, 2017
Patent Number: 9574236
Provided herein is a method of sample analysis. In certain embodiments, the method comprises: a) cross-linking protein of a cell using a first compound to produce a first cross-linked product comprising cross-linked protein, and RNA; b) contacting the first cross-linked product and a second compound under conditions by which an oligonucleotide portion of the second compound hybridizes to the RNA; c) activating a reaction the first and second compound, thereby covalently crosslinking the…

Data processing for multiplexed spectrometry

Granted: February 21, 2017
Patent Number: 9576778
Multiplexed spectrometry, such as multiplexed ion mobility spectrometry (IMS), time-of-flight mass spectrometry (TOFMS), or hybrid IM-TOFMS, is carried out on a sample, and the resulting measurement data are deconvoluted. Noise may be removed from the measurement data prior to deconvolution. Alternatively or additionally, noise may be removed from the deconvoluted data.

Polymerase idling method for single molecule DNA sequencing

Granted: January 17, 2017
Patent Number: 9546398
A method for sequencing a nucleic acid is provided. In certain embodiments the method comprises obtaining a duplex comprising a nucleic acid and a primer, wherein the primer has a nuclease resistant 3? end, combining the duplex with a chain terminator nucleotide and a proof-reading polymerase to produce a reaction in which the polymerase idles on the added chain terminator nucleotide, identifying the chain terminator nucleotide added to the end of the primer; and adding a…

Mid-infrared scanning system that differentiates between specular and diffuse scattering

Granted: January 17, 2017
Patent Number: 9546905
A method and apparatus for measuring diffuse and specularly reflected light from a sample to provide a reflection spectrum as a function of wavelength and as a function of position on a sample is disclosed. The apparatus includes a MIR light source that generates an illumination beam of linearly polarized light. An illumination system illuminates a location on a specimen with part of the illumination beam. A linear polarization filter characterized by a polarization axis that defines a…

Sample droplet generation from segmented fluid flow and related devices and methods

Granted: January 10, 2017
Patent Number: 9543137
A sample droplet generator transforms a segmented array of sample material into a continuous stream of droplets containing analytes. The droplets may serve as a sample source for a wide range of detectors and analytical instruments. As one example, the droplets may be introduced into an ion source of a spectrometer that measures ions produced from the droplets or photons emitted from the droplets.

Thin field terminator for linear quadrupole ion guides, and related systems and methods

Granted: January 3, 2017
Patent Number: 9536723
A field terminator includes a plurality of electrode plates positioned around a guide axis at a radial distance therefrom. The plates generate a quadrupole DC field such that a polarity on each plate is opposite to a polarity on the plates adjacent thereto. The plates may be positioned at an axial end of a quadrupole ion guide such as a mass filter. In addition to an RF field, the ion guide may generate a quadrupole DC field. The DC field of the plates may be opposite in polarity to that…

Method for isomer discrimination by tandem mass spectrometry

Granted: December 27, 2016
Patent Number: 9530633
Systems and method for mass spectrometry are presented. In one embodiment, a method comprises: (a) acquiring one or more fragmentation signatures for a reference sample, wherein each fragmentation signature of the reference sample is acquired with a unique tandem mass spectrometry mode; (b) identifying one or more discriminate features across the plurality of fragmentation signatures of the reference sample; (c) acquiring one or more fragmentation signatures for an unknown sample,…

Thermostable type-A DNA polymerase mutants with increased polymerization rate and resistance to inhibitors

Granted: December 20, 2016
Patent Number: 9523085
The present invention provides mutants of DNA polymerases having an increased rate of incorporation of nucleotides into nucleic acids undergoing polymerization and having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for fast PCR applications, for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases, and for fast PCR amplification of samples containing DNA polymerase inhibitors. In exemplary…

Compositions and methods for conjugating oligonucleotides

Granted: December 6, 2016
Patent Number: 9512163
An oligonucleotide derivative having the structure of formula (A) and methods for preparing the oligonucleotide derivative are disclosed. wherein R3 is a first oligonucleotide; R1 is selected from the group consisting of alkyl, cycloalkyl, aryl, heterocyclyl, heteroaryl, a polyethylene glycol, a peptide, a protein, a polysaccharide, and a second oligonucleotide; R2 is a linker or a direct bond; Z1 is NR4, S, or O, and Z2 is NR4 or S, wherein R4 is selected from H, alkyl, aryl,…

Pressurizable injection seat

Granted: December 6, 2016
Patent Number: 9513265
An injection device comprising: a housing defining a space; a seat located in said space, the seat being configured for receiving a flow path element capable of injecting a pressurized fluid into the injection device through the seat; a pressure source for pressurizing a medium in said space, thereby reducing the pressure difference between the pressurized fluid in said flow path element and said medium.

Methods and compositions for improved ion-exchange chromatography

Granted: November 29, 2016
Patent Number: 9506897
The invention generally relates to ion-exchange chromatography. More particularly, the invention relates to compositions and methods that can substantially improve analytical sensitivity and/or selectivity of ion-exchange chromatography as well its efficiency and quality as a purification or preparation tool.

Fitting element with bio-compatible sealing

Granted: November 15, 2016
Patent Number: 9494563
A fitting element is configured for coupling tubing to a fluidic device having a receiving cavity configured for receiving the fitting element, where the tubing has an inner contact surface of a biocompatible material, the inner contact surface being configured to contact a fluid to be conducted by the tubing, and the receiving cavity having a receiving contact surface of a bio-compatible material. The fitting element includes a first sealing element of a bio-compatible material…

Fast method for measuring collision cross section of ions utilizing ion mobility spectrometry

Granted: November 1, 2016
Patent Number: 9482642
The collision cross section (CCS) of a sample ion may be calculated by measuring a total drift time taken by the sample ion to travel through an ion mobility spectrometry drift cell to an ion detector. The CCS may be calculated based on the total drift time measured, and on a proportionality coefficient that defines the time taken by the sample ion to travel through a mobility dominated region between the drift cell and the detector. The proportionality coefficient may be determined from…

Fitting for elastically-biasing a capillary for a fluid-tight connection to a fluidic conduit

Granted: November 1, 2016
Patent Number: 9482649
A fitting (200) for providing a fluid connection between a capillary (202) and a fluidic conduit (204) of a fluidic component (30), the fitting (200) comprising a male piece (240) and a female piece (250) for connection with the male piece (240), wherein the male piece (240) comprises a housing (252) with a capillary reception (212) configured for receiving the capillary (202), wherein a part of the capillary (202) being received in the capillary reception (212) is circumferentially…

Chromatographic system and method of isolating compound in sample using chromatographic system

Granted: October 11, 2016
Patent Number: 9465016
A method is provided for isolating a compound in a sample by chromatography. The method includes determining an analytical gradient of an analytical system; performing an analytical run using the analytical gradient; identifying a target compound in the sample and determining an analytical retention time of the target compound using chromatographic results of the analytical run; determining an elution point of the target compound using the analytical retention time, the analytical…

Intake monitoring for accurate proportioning

Granted: October 4, 2016
Patent Number: 9459239
A fluid supply system configured for metering two or more fluids in controlled proportions, including a plurality of solvent supply lines, a pumping unit configured for taking in fluids from selected solvent supply lines and for supplying a pressurized mixture, a proportioning valve configured for modulating solvent composition by sequentially coupling selected ones of the solvent supply lines with the inlet of the pumping unit, a sensor configured for sensing process information in the…