Agilent Technologies Patent Grants

Polymerase idling method for single molecule DNA sequencing

Granted: January 17, 2017
Patent Number: 9546398
A method for sequencing a nucleic acid is provided. In certain embodiments the method comprises obtaining a duplex comprising a nucleic acid and a primer, wherein the primer has a nuclease resistant 3? end, combining the duplex with a chain terminator nucleotide and a proof-reading polymerase to produce a reaction in which the polymerase idles on the added chain terminator nucleotide, identifying the chain terminator nucleotide added to the end of the primer; and adding a…

Mid-infrared scanning system that differentiates between specular and diffuse scattering

Granted: January 17, 2017
Patent Number: 9546905
A method and apparatus for measuring diffuse and specularly reflected light from a sample to provide a reflection spectrum as a function of wavelength and as a function of position on a sample is disclosed. The apparatus includes a MIR light source that generates an illumination beam of linearly polarized light. An illumination system illuminates a location on a specimen with part of the illumination beam. A linear polarization filter characterized by a polarization axis that defines a…

Sample droplet generation from segmented fluid flow and related devices and methods

Granted: January 10, 2017
Patent Number: 9543137
A sample droplet generator transforms a segmented array of sample material into a continuous stream of droplets containing analytes. The droplets may serve as a sample source for a wide range of detectors and analytical instruments. As one example, the droplets may be introduced into an ion source of a spectrometer that measures ions produced from the droplets or photons emitted from the droplets.

Thin field terminator for linear quadrupole ion guides, and related systems and methods

Granted: January 3, 2017
Patent Number: 9536723
A field terminator includes a plurality of electrode plates positioned around a guide axis at a radial distance therefrom. The plates generate a quadrupole DC field such that a polarity on each plate is opposite to a polarity on the plates adjacent thereto. The plates may be positioned at an axial end of a quadrupole ion guide such as a mass filter. In addition to an RF field, the ion guide may generate a quadrupole DC field. The DC field of the plates may be opposite in polarity to that…

Method for isomer discrimination by tandem mass spectrometry

Granted: December 27, 2016
Patent Number: 9530633
Systems and method for mass spectrometry are presented. In one embodiment, a method comprises: (a) acquiring one or more fragmentation signatures for a reference sample, wherein each fragmentation signature of the reference sample is acquired with a unique tandem mass spectrometry mode; (b) identifying one or more discriminate features across the plurality of fragmentation signatures of the reference sample; (c) acquiring one or more fragmentation signatures for an unknown sample,…

Thermostable type-A DNA polymerase mutants with increased polymerization rate and resistance to inhibitors

Granted: December 20, 2016
Patent Number: 9523085
The present invention provides mutants of DNA polymerases having an increased rate of incorporation of nucleotides into nucleic acids undergoing polymerization and having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for fast PCR applications, for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases, and for fast PCR amplification of samples containing DNA polymerase inhibitors. In exemplary…

Compositions and methods for conjugating oligonucleotides

Granted: December 6, 2016
Patent Number: 9512163
An oligonucleotide derivative having the structure of formula (A) and methods for preparing the oligonucleotide derivative are disclosed. wherein R3 is a first oligonucleotide; R1 is selected from the group consisting of alkyl, cycloalkyl, aryl, heterocyclyl, heteroaryl, a polyethylene glycol, a peptide, a protein, a polysaccharide, and a second oligonucleotide; R2 is a linker or a direct bond; Z1 is NR4, S, or O, and Z2 is NR4 or S, wherein R4 is selected from H, alkyl, aryl,…

Pressurizable injection seat

Granted: December 6, 2016
Patent Number: 9513265
An injection device comprising: a housing defining a space; a seat located in said space, the seat being configured for receiving a flow path element capable of injecting a pressurized fluid into the injection device through the seat; a pressure source for pressurizing a medium in said space, thereby reducing the pressure difference between the pressurized fluid in said flow path element and said medium.

Methods and compositions for improved ion-exchange chromatography

Granted: November 29, 2016
Patent Number: 9506897
The invention generally relates to ion-exchange chromatography. More particularly, the invention relates to compositions and methods that can substantially improve analytical sensitivity and/or selectivity of ion-exchange chromatography as well its efficiency and quality as a purification or preparation tool.

Fitting element with bio-compatible sealing

Granted: November 15, 2016
Patent Number: 9494563
A fitting element is configured for coupling tubing to a fluidic device having a receiving cavity configured for receiving the fitting element, where the tubing has an inner contact surface of a biocompatible material, the inner contact surface being configured to contact a fluid to be conducted by the tubing, and the receiving cavity having a receiving contact surface of a bio-compatible material. The fitting element includes a first sealing element of a bio-compatible material…

Fast method for measuring collision cross section of ions utilizing ion mobility spectrometry

Granted: November 1, 2016
Patent Number: 9482642
The collision cross section (CCS) of a sample ion may be calculated by measuring a total drift time taken by the sample ion to travel through an ion mobility spectrometry drift cell to an ion detector. The CCS may be calculated based on the total drift time measured, and on a proportionality coefficient that defines the time taken by the sample ion to travel through a mobility dominated region between the drift cell and the detector. The proportionality coefficient may be determined from…

Fitting for elastically-biasing a capillary for a fluid-tight connection to a fluidic conduit

Granted: November 1, 2016
Patent Number: 9482649
A fitting (200) for providing a fluid connection between a capillary (202) and a fluidic conduit (204) of a fluidic component (30), the fitting (200) comprising a male piece (240) and a female piece (250) for connection with the male piece (240), wherein the male piece (240) comprises a housing (252) with a capillary reception (212) configured for receiving the capillary (202), wherein a part of the capillary (202) being received in the capillary reception (212) is circumferentially…

Chromatographic system and method of isolating compound in sample using chromatographic system

Granted: October 11, 2016
Patent Number: 9465016
A method is provided for isolating a compound in a sample by chromatography. The method includes determining an analytical gradient of an analytical system; performing an analytical run using the analytical gradient; identifying a target compound in the sample and determining an analytical retention time of the target compound using chromatographic results of the analytical run; determining an elution point of the target compound using the analytical retention time, the analytical…

Intake monitoring for accurate proportioning

Granted: October 4, 2016
Patent Number: 9459239
A fluid supply system configured for metering two or more fluids in controlled proportions, including a plurality of solvent supply lines, a pumping unit configured for taking in fluids from selected solvent supply lines and for supplying a pressurized mixture, a proportioning valve configured for modulating solvent composition by sequentially coupling selected ones of the solvent supply lines with the inlet of the pumping unit, a sensor configured for sensing process information in the…

Optical system having reduced pointing-error noise

Granted: October 4, 2016
Patent Number: 9461443
An apparatus having a variable angle light source characterized by a pivot point, a variable response optical receiver, and a first optical system is disclosed. The variable response optical receiver receives light generated by the light source on a receiving surface, the receiver generating a signal indicative of an intensity of light that impinges on a receiving surface. The first optical system images the pivot point to a fixed point relative to the receiver surface. In one aspect of…

Intramedullary fixation assembly and devices and methods for installing the same

Granted: September 27, 2016
Patent Number: 9451971
An intramedullary fixation assembly usable with different long bone types and a guide assembly for guiding deployment of the intramedullary fixation assembly. The intramedullary fixation assembly includes a fixation member that has ends and a curved body extending between the ends. The curved body of the fixation member has a radius of curvature configured to extend through the medullary canal regardless of the long bone anatomy. Fasteners fix the fixation member to the bone fragments…

Ion mobility spectrometry-mass spectrometry (IMS-MS) with improved ion transmission and IMS resolution

Granted: September 27, 2016
Patent Number: 9455132
An interface for an ion mobility spectrometry-mass spectrometry (IMS-MS) system includes a first ion guide for receiving ions from an IMS drift cell, and a second ion guide for receiving ions from the first ion guide, and positioned in a chamber separate from the first ion guide. Electrodes of the second ion guide subject the ions to an axial DC electric field while the second ion guide is held at a lower pressure than the first ion guide. In some embodiments, the first ion guide may be…

Flame photometric detector

Granted: September 20, 2016
Patent Number: 9448177
A flame photometric detector (FPD) for use in a gas chromatography (GC) apparatus is described. The FPD has an emission block that is maintained in a first temperature range, and a transfer line that is maintained in a second temperature range that is greater than the first temperature range. The FPD is coupled to a light detector, such as a photomultiplier tube (PMT).

Dual field multipole converging ion guides, hyperbolic ion guides, and related methods

Granted: September 20, 2016
Patent Number: 9449804
An ion guide generates a first RF field of Nth order where N is an integer equal to or greater than 2, and a second RF field of 2Nth order superimposed on the first RF field. The first and second RF fields may be generated by respective first and second sets of electrodes. Another ion guide may include a converging entrance section followed by an exit section. The converging section may have a hyperbolic profile. A hyperbolic profile may be presented by electrodes having a twisted…

Isolation of charged particle optics from vacuum chamber deformations

Granted: September 20, 2016
Patent Number: 9449805
A charged particle processing apparatus includes a vacuum chamber, an optics plate, charged particle optics mounted to the optics plate, and mounting members coupled between the optics plate and a chamber wall. The mounting members are configured for isolating the optics plate from deformation of the chamber wall, as may occur due to a pressure differential between the chamber interior and the environment outside the chamber. The isolation may prevent deformation from affecting the…