Codexis Patent Grants

Synthesis of prazole compounds

Granted: April 25, 2017
Patent Number: 9631181
The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.

Biocatalysts and methods for the synthesis of substituted lactams

Granted: April 18, 2017
Patent Number: 9624478
The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.

Enone reductases

Granted: April 11, 2017
Patent Number: 9617568
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.

Engineered transaminase polypeptides for industrial biocatalysis

Granted: April 11, 2017
Patent Number: 9617573
The present disclosure provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The disclosure also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.

Endoglucanase 1B (EG1B) variants

Granted: April 4, 2017
Patent Number: 9611462
The present invention provides endoglucanase 1b (EG1b) variants suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce EG1b variants, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.

Engineered phenylalanine ammonia lyase polypeptides

Granted: April 4, 2017
Patent Number: 9611468
The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.

Pentose fermentation by a recombinant microorganism

Granted: April 4, 2017
Patent Number: 9611515
The present invention provides methods and compositions suitable for use in the isomerization of xylose to xylulose, as well as methods and compositions suitable for use in the conversion of xylose to xylitol and xylulose, including nucleic acid constructs, recombinant fungal host cells, and related materials.

Engineered tyrosine ammonia lyase

Granted: March 28, 2017
Patent Number: 9605252
The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.

Biocatalytic process for preparing eslicarbazepine and analogs thereof

Granted: March 28, 2017
Patent Number: 9605290
The present disclosure relates to biocatalysts and its uses for the efficient preparation of eslicarbazepine, eslicarbazepine acetate, and analogs thereof.

Protein variant generation by region shuffling

Granted: March 14, 2017
Patent Number: 9593326
Region shuffling methods for efficiently introducing diversity and exploring sequence space are described. Libraries produced directly from these methods contain high fractions of protein variants harboring multiple beneficial mutations. Typically, the methods produce these variants efficiently without the need for sequencing beneficial mutants identified at intermediate stages of the process.

Ketoreductase polypeptides for the production of azetidinone

Granted: February 28, 2017
Patent Number: 9580694
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

Transaminase biocatalysts

Granted: January 24, 2017
Patent Number: 9550982
The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.

Variant LovD polypeptides and their uses

Granted: January 17, 2017
Patent Number: 9546388
The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound.

Engineered biocatalysts useful for carbapenem synthesis

Granted: January 10, 2017
Patent Number: 9540622
The present disclosure provides engineered pNB esterase polypeptides useful for the synthesis of the carbapenem antibiotic, imipenem. The disclosure also provides polynucleotides encoding the engineered pNB esterases, host cells capable of expressing the engineered pNB esterases, and methods of using the engineered pNB esterases in the production of imipenem.

Biocatalysts and methods for the synthesis of armodafinil

Granted: December 27, 2016
Patent Number: 9528095
The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (?)-2-[(R)-(diphenylmethyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid,…

Fungal strains

Granted: December 27, 2016
Patent Number: 9528098
The present invention provides improved fungal strains. In some embodiments, the improved fungal strains find use in hydrolyzing cellulosic material to glucose.

Methods of using ketoreductase polypeptides for reduction of acetophenones

Granted: December 27, 2016
Patent Number: 9528131
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

Engineered transaminase polypeptides

Granted: December 6, 2016
Patent Number: 9512410
The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.

Endoglucanase variants

Granted: December 6, 2016
Patent Number: 9512416
The present invention relates to variant endoglucanases having improved thermoactivity, improved thermostability, and improved viscosity reduction activity over wild-type M. thermophila endoglucanase.

Modified native beta-ketoacyl-ACP synthases and engineered microorganisms

Granted: December 6, 2016
Patent Number: 9512447
Genetically engineered cells and microorganisms are provided that produce fatty alcohols and fatty acids. In particular, engineered microbial cells comprise a modified native gene having ?-ketoacyl-acp synthase activity.