Codexis Patent Grants

P450-BM3 variants with improved activity

Granted: July 18, 2017
Patent Number: 9708587
The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.

Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs

Granted: July 18, 2017
Patent Number: 9708588
The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.

Polynucleotides encoding engineered imine reductases

Granted: July 4, 2017
Patent Number: 9695451
The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

P450-BM3 variants with improved activity

Granted: June 20, 2017
Patent Number: 9683220
The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.

Methods, systems, and software for identifying bio-molecules using models of multiplicative form

Granted: June 20, 2017
Patent Number: 9684771
The present invention provides methods for identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. More specifically, some embodiments of the present invention provide methods for building sequence-activity models comprising multiplicative terms and using the models to guide directed evolution. In some embodiments, the sequence-activity models include one or more…

Low-phosphate repressible promoter

Granted: June 6, 2017
Patent Number: 9670493
The present invention provides compositions and methods comprising a low-phosphate repressible promoter. In particular, the present invention provides a low-phosphate repressible promoter from E. coli.

Engineered biocatalysts useful for carbapenem synthesis

Granted: May 30, 2017
Patent Number: 9663771
The present disclosure provides engineered pNB esterase polypeptides useful for the synthesis of the carbapenem antibiotic, imipenem. The disclosure also provides polynucleotides encoding the engineered pNB esterases, host cells capable of expressing the engineered pNB esterases, and methods of using the engineered pNB esterases in the production of imipenem.

Methods, systems, and software for identifying bio-molecules with interacting components

Granted: May 30, 2017
Patent Number: 9665694
The present invention provides methods for rapidly and efficiently searching biologically-related data space. More specifically, the present invention provides methods for identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The present invention also provides methods for modeling sequence-activity relationships, including but not limited to stepwise addition or…

Engineered ketoreductase polypeptides

Granted: May 23, 2017
Patent Number: 9657320
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the…

Production of fatty alcohols from engineered microorganisms

Granted: May 16, 2017
Patent Number: 9650655
Recombinant microorganisms are provided which have been engineered to produce fatty alcohols. Also provided are recombinant microorganisms which comprise a heterologous polynucleotide encoding a fatty alcohol reductase enzyme and an introduced polynucleotide encoding a ?-ketoacyl acyl carrier protein synthase.

Biocatalysts for ezetimibe synthesis

Granted: May 9, 2017
Patent Number: 9644189
The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.

Synthesis of prazole compounds

Granted: April 25, 2017
Patent Number: 9631181
The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.

Biocatalysts and methods for the synthesis of substituted lactams

Granted: April 18, 2017
Patent Number: 9624478
The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.

Enone reductases

Granted: April 11, 2017
Patent Number: 9617568
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.

Engineered transaminase polypeptides for industrial biocatalysis

Granted: April 11, 2017
Patent Number: 9617573
The present disclosure provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The disclosure also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.

Endoglucanase 1B (EG1B) variants

Granted: April 4, 2017
Patent Number: 9611462
The present invention provides endoglucanase 1b (EG1b) variants suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce EG1b variants, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.

Engineered phenylalanine ammonia lyase polypeptides

Granted: April 4, 2017
Patent Number: 9611468
The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.

Pentose fermentation by a recombinant microorganism

Granted: April 4, 2017
Patent Number: 9611515
The present invention provides methods and compositions suitable for use in the isomerization of xylose to xylulose, as well as methods and compositions suitable for use in the conversion of xylose to xylitol and xylulose, including nucleic acid constructs, recombinant fungal host cells, and related materials.

Engineered tyrosine ammonia lyase

Granted: March 28, 2017
Patent Number: 9605252
The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.

Biocatalytic process for preparing eslicarbazepine and analogs thereof

Granted: March 28, 2017
Patent Number: 9605290
The present disclosure relates to biocatalysts and its uses for the efficient preparation of eslicarbazepine, eslicarbazepine acetate, and analogs thereof.