PEPTIDES, DEVICES, AND METHODS FOR THE DETECTION OF EHRLICHIA ANTIBODIES
Granted: May 1, 2014
Application Number:
20140121125
The invention provides peptide compositions and mixtures useful for the detection of antibodies that bind to Ehrlichia antigens. The peptide compositions and mixtures comprise polypeptide sequences based on an immunogenic fragment of the Ehrlichia Outer Membrane Protein 1 (OMP-1) protein. The invention also provides devices, methods, and kits comprising such peptide compositions and mixtures useful for the detection of antibodies that bind to Ehrlichia antigens and the diagnosis of…
SIGNAL AMPLIFICATION IN LATERAL FLOW AND RELATED IMMUNOASSAYS
Granted: May 23, 2013
Application Number:
20130130404
The present invention provides methods, devices, compositions (e.g., capture complexes), and kits useful for enhancing the detection of antibodies in a test sample. The methods, devices, and compositions utilize detectable Fc-binding molecules such as Protein A, Protein G, and/or an Fc-specific antibody to amplify the signal of a detected antibody in immunoassays, such as lateral flow assays.
PEPTIDES AND METHODS FOR THE DETECTION OF LYME DISEASE ANTIBODIES
Granted: May 9, 2013
Application Number:
20130115634
The invention provides compositions (e.g., peptide compositions) useful for the detection of antibodies that bind to Borrelia antigens. The peptide compositions comprise polypeptide sequences comprising variants in the IR6 domain of the Borrelia VlsE protein. The invention also provides devices, methods, and kits comprising such peptide compositions and useful for the detection of antibodies that bind to Borrelia antigens and the diagnosis of Lyme disease.
MULTI-WAVELENGTH ANALYSES OF SOL-PARTICLE SPECIFIC BINDING ASSAYS
Granted: October 4, 2012
Application Number:
20120252005
The present invention discloses methods for detecting the presence of a complex between a first reagent and a second reagent in solution. In particular, the invention provides methods for qualitative or quantitative detection of an analyte or its specific binding partner in complex biological samples. The invention further discloses algorithms using summary rate changes at selected wavelengths in the absorbance spectra of colloidal metal-labeled analytes or specific binding partners to…
VALIDATION OF POINT-OF-CARE TEST RESULTS BY ASSESSMENT OF EXPECTED ANALYTE RELATIONSHIPS
Granted: November 10, 2011
Application Number:
20110276342
Methods for determining the validity or accuracy of a clinical test result are provided. The methods entail obtaining patient analyte concentration data for two or more analytes with a concentration relationship; calculating a likelihood distribution for the concentration relationship between the two or more analytes; obtaining a clinical test result comprising measured concentration values for the two or more analytes; and determining the validity or accuracy of the clinical test result…
NOVEL ASSAYS FOR DETECTING ANALYTES IN SAMPLES AND KITS AND COMPOSITIONS RELATED THERETO
Granted: June 23, 2011
Application Number:
20110151435
The present invention provides methods of detecting analytes using particles having different physico-chemical properties, such as buoyancy, size, density, spectral characteristics, and/or binding properties, in solution-based sandwich assays and solution-based competition assays. The methods can be performed using rotors and bench-top centrifuges and provide for rapid, qualitative and quantitative detection of analytes. The present invention also provides kits that can be used to…
PEPTIDES AND METHODS FOR THE DETECTION OF LYME DISEASE ANTIBODIES
Granted: June 9, 2011
Application Number:
20110136155
The invention provides compositions (e.g., peptide compositions) useful for the detection of antibodies that bind to Borrelia antigens. The peptide compositions comprise polypeptide sequences comprising variants in the IR6 domain of the Borrelia VlsE protein. The invention also provides devices, methods, and kits comprising such peptide compositions and useful for the detection of antibodies that bind to Borrelia antigens and the diagnosis of Lyme disease.
PEPTIDES, DEVICES, AND METHODS FOR THE DETECTION OF EHRLICHIA ANTIBODIES
Granted: May 26, 2011
Application Number:
20110124125
The invention provides compositions (e.g., peptide compositions) useful for the detection of antibodies that bind to Ehrlichia antigens. The peptide compositions comprise polypeptide sequences based on an immunogenic fragment of the Ehrlichia Outer Membrane Protein 1 (OMP-1) protein. The invention also provides devices, methods, and kits comprising such peptide compositions and useful for the detection of antibodies that bind to Ehrlichia antigens and the diagnosis of monocytic…
SPLIT FLOW DEVICE FOR ANALYSES OF SPECIFIC-BINDING PARTNERS
Granted: September 16, 2010
Application Number:
20100233708
The present invention provides an analyte detection system for detecting a target analyte in a sample. In particular, the invention provides a detection system capable of one-step amplification of the detection signal by incorporating a secondary flow path that can deliver reagents to a reaction zone. Methods of using the detection system to detect analytes in samples, particularly biological samples, and kits comprising the detection system are also disclosed.
MULTI-WAVELENGTH ANALYSES OF SOL-PARTICLE SPECIFIC BINDING ASSAYS
Granted: June 24, 2010
Application Number:
20100159441
The present invention discloses methods for detecting the presence of a complex between a first reagent and a second reagent in solution. In particular, the invention provides methods for qualitative or quantitative detection of an analyte or its specific binding partner in complex biological samples. The invention further discloses algorithms using summary rate changes at selected wavelengths in the absorbance spectra of colloidal metal-labeled analytes or specific binding partners to…
LATERAL FLOW STRIP ASSAY WITH IMMOBILIZED CONJUGATE
Granted: June 3, 2010
Application Number:
20100136566
The present invention discloses analyte detection devices for detecting one or more analytes present in test samples, especially biological samples. In particular, the devices of the invention are lateral flow assay devices comprising immobilized metal nanoparticle conjugates as the detection means. Methods of using the devices and kits comprising the devices are also described.
METHODS OF REDUCING NON-SPECIFIC INTERACTION IN METAL NANOPARTICLE ASSAYS
Granted: May 13, 2010
Application Number:
20100120057
The present invention discloses methods of reducing non-specific interactions of interfering species present in a sample in metallic nanoparticle-based assays, thereby increasing the sensitivity of these assays. In particular, the methods entail neutralizing the chemical reactivity of functional groups present in interfering species by addition of a neutralizing agent, such as an alkylating agent or heavy metal ion. The methods are especially useful in assays for the detection of…
Sodium activation of amylase
Granted: January 30, 2003
Application Number:
20030022264
Chloride ion and sodium ion determination methods, compositions, and assays are provided which are based on the use of sodium ion as an activator for &agr;-amylase. Chloride ion and sodium ion determination are performed by colorimetry, using measurements of &agr;-amylase activity to indirectly measure the desired ion concentrations. One preferred composition for chloride ion determination comprises &agr;-amylase that is substantially calcium free, sodium ion in higher concentration than…
