Label free analysis of nucleic acids
Granted: April 8, 2008
Patent Number:
7354720
A method is provided for label free analysis of nucleic acid materials. In accordance with the present invention, a fluorescent material is provided having a certain fluorescence emission without nucleic acids attached thereto. The fluorescent material of the present invention, has a greater emission upon the binding of a single stranded nucleic acid and an even greater emission upon the binding of a double stranded nucleic acid allowing detection of double stranded binding without using…
Methods of analysis of alternative splicing in mouse
Granted: March 11, 2008
Patent Number:
7341835
The invention provides nucleic acid sequences which are complementary, in one embodiment, to a wide variety of mouse genes. The invention provides the sequences in such a way as to make them available for a variety of analyses. In one embodiment the nucleic acid sequences provided are present as an array of probes that may be used to measure gene expression of different mature RNA isoforms from at least 5,000 alternatively spliced mouse genes. As such, the invention relates to diverse…
Polymorphisms in human mitochondrial DNA
Granted: March 4, 2008
Patent Number:
7339049
The invention provides novel human mitochondrial polymorphisms, and probes and primers for detecting the same. Detection of such polymorphisms is useful in a variety of fields such as forensic analysis, epidemiology and preventive medicine.
Antireflective coatings for high-resolution photolithographic synthesis of DNA arrays
Granted: February 19, 2008
Patent Number:
7332273
The present invention provides an array of polymers and methods of forming arrays of polymers by providing a substrate having a first layer including one or more dielectric coatings on a solid support and a second layer including a plurality of polymers disposed on the first layer. The invention also provides methods for forming an array of polymers on a substrate using light-directed synthesis by providing a substrate having a first layer including one or more dielectric coatings on a…
Sequencing of surface immobilized polymers utilizing microflourescence detection
Granted: February 12, 2008
Patent Number:
7329496
Means for simultaneous parallel sequence analysis of a large number of biological polymer macromolecules. Apparatus and methods may use fluorescent labels in repetitive chemistry to determine terminal monomers on solid phase immobilized polymers. Reagents which specifically recognize terminal monomers are used to label polymers at defined positions on a solid substrate.
Identification of molecular sequence signatures and methods involving the same
Granted: January 29, 2008
Patent Number:
7323307
Novel means and methods for analyzing hybridization data derived from hybridization assays between a target nucleic acid and differently sequenced polynucleotide probes involve selecting probe sets that define reference sequences for sequence signatures and deriving useful data about the nature of the target nucleic acid molecule based on its hybridization to the probes. The methods are useful for determining whether the target contains a nucleic acid or polypeptide sequence signature,…
Methods of genetic analysis of
Granted: January 29, 2008
Patent Number:
7323308
The invention provides nucleic acid sequences which are complementary, in one embodiment, to a wide variety of E. coli genes. The invention provides the sequences in such a way as to make them available for a variety of analyses. In one embodiment the nucleic acid sequences provided are present as an array of probes that may be used to measure gene expression of at least 20,000 E. coli genes and at least 500 intragenic regions. As such, the invention relates to diverse fields impacted by…
System, method, and product for scanning of biological materials employing dual analog integrators
Granted: January 8, 2008
Patent Number:
7317415
In one embodiment, a system is described for reducing a dark period between successive data acquisition periods associated with the detection of one or more target molecules hybridized to at least one of a plurality of probes disposed upon a biological probe array. The system comprises a switch that alternately directs an analog signal between a first circuit and a second circuit, where a period of time is required to alternate between the first and second circuits; an integrator…
Addressable oligonucleotide array of the rat genome
Granted: January 1, 2008
Patent Number:
7314750
The invention provides nucleic acid sequences which are complementary, in one embodiment, to a wide variety of Rat genes. The invention provides the sequences in such a way as to make them available for a variety of analyses. In one embodiment the nucleic acid sequences provided are present as an array of probes that may be used to measure gene expression of at least 20,000 rat genes. As such, the invention relates to diverse fields impacted by the nature of molecular interaction,…
Method of oligonucleotide synthesis
Granted: January 1, 2008
Patent Number:
7314714
Methods and kits for synthesizing a plurality of oligonucleotides are provided. Methods for providing a plurality of oligonucleotides enriched for full length oligonucleotides are provided. Truncated oligonucleotides are preferentially removed from the sample by digestion. Methods are also provided for amplification of a plurality of oligonucleotides.
High throughput microarray spotting system and method
Granted: January 1, 2008
Patent Number:
7314595
A system is described for automatic retrieval of microplates from a carousel. The system includes an effector arm that retrieves a selected microplate from the carousel, a microplate retainer that receives the selected microplate from the effector arm, and a controller that directs the effector arm to the carousel for retrieval of the selected microplate and directs the effector arm to the microplate retainer so that it may receive the selected microplate. The carousel may revolve around…
Methods of genetic analysis of yeast
Granted: December 25, 2007
Patent Number:
7312035
Arrays of oligonucleotide probes which are complementary to a plurality of S. cerevisisae and S. pombe genes are disclosed. The arrays may be used to measure the expression levels of a plurality of genes simultaneously.
Wide field of view and high speed scanning microscopy
Granted: December 25, 2007
Patent Number:
7312919
A wide field of view scanning microscope includes a light source, a light detector, optics, a scanning assembly, a translation system, and a data collection control and processing unit. The scanning assembly is constructed to provide from the light source a light beam in a scanning motion to an examined surface. The optics includes an objective lens associated with the scanning assembly arranged to provide an optical path to the light detector. The translation system is constructed to…
Method for genotyping polymorphisms in humans
Granted: November 27, 2007
Patent Number:
7300788
The invention provides nucleic acid sequences that are complementary, in one embodiment, to a wide variety of human polymorphisms. The invention provides the sequences in such a way as to make them available for a variety of analyses including genotyping a large number of SNPs in parallel. The invention also provides a collection of human SNPs that is useful for genetic analysis within and across populations. As such, the invention relatesd to diverse fields impacted by the nature of…
Complexity management of genomic DNA
Granted: November 20, 2007
Patent Number:
7297778
The presently claimed invention provides for novel methods and kits for analyzing a collection of target sequences in a nucleic acid sample. A sample is amplified under conditions that enrich for a subset of fragments that includes a collection of target sequences. The invention further provides for analysis of the above sample by hybridization to an array, which may be specifically designed to interrogate the collection of target sequences for particular characteristics, such as, for…
Nucleic acid labeling compounds
Granted: November 6, 2007
Patent Number:
7291463
A method for converting a pseudoisocytidine base having the formula: to a pseudoisocytidine molecule having the formula: is disclosed. Such compounds are useful as nucleic acid labeling compounds.
Nucleic acid labeling compounds
Granted: October 16, 2007
Patent Number:
7282327
Compounds of the following structure: are useful as nucleic labeling compounds.
System, method, and computer software for genotyping analysis and identification of allelic imbalance
Granted: October 9, 2007
Patent Number:
7280922
Methods, systems and computer software products are provided for determining genotype of a sample using a plurality of probes. In one preferred embodiment, a tentative genotype call is made based upon the relative allele signals. Pattern recognition is then used to validate the tentative call.
Complexity management and analysis of genomic DNA
Granted: September 11, 2007
Patent Number:
7267966
The present invention provides for novel methods of sample preparation and analysis involving reproducibly reducing the complexity of a nucleic sample. The invention further provides for analysis of the above sample by hybridization to an array which may be specifically designed to interrogate the desired fragments for particular characteristics, such as, for example, the presence or absence of a polymorphism. The invention further provides for novel methods of using a computer system to…
Chip-based speciation and phenotypic characterization of microorganisms
Granted: August 7, 2007
Patent Number:
7252948
This invention provides oligonucleotide based arrays and methods for speciating and phenotyping organisms, for example, using oligonucleotide sequences based on the Mycobacterium tuberculosis rpoB gene. The groups or species to which an organism belongs may be determined by comparing hybridization patterns of target nucleic acid from the organism to hybridization patterns in a database.
