Solutions of Quaternary and Tertiary Ammonium Cation Detergents to Denature Proteins, and Kits for Making Them
Granted: December 2, 2021
Application Number:
20210371454
Ammonium cation detergents comprising a quaternary or tertiary ammonium cation can be used as detergents to denature proteins and are particularly useful in denaturing glycoproteins or glycopeptides prior to enzymatic deglycosylation. Ammonium cation detergents with sulfate or sulfonate anions are particularly useful.
Kits for Using Bispyridines to Improve Labeling of Nucleophiles
Granted: October 28, 2021
Application Number:
20210333269
Bispyridines improve the labeling of nucleophiles, including amines and thiols and are particularly useful for improving labeling with acidic and basic labels. Use of bispyridines with such labels dramatically increases labeling compared to protocols without a bispyridine. The labeled nucleophile can then be subjected to standard analytical methods.
Use of Mild Electrophiles to Reduce Artifacts in Analyzing Glycans Released from Glycoproteins or Glycopeptides
Granted: August 26, 2021
Application Number:
20210263043
The presence of mild electrophiles, such as aldehydes, during the denaturation of glycoproteins or glycopeptides and subsequent enzymatic deglycosylation reduces artifacts in subsequent analyses of the glycans released from the glycoproteins or gly-copeptides.
METHODS FOR LABELING AMINE-CONTAINING COMPOUNDS
Granted: July 22, 2021
Application Number:
20210223253
The invention relates to an activated form of procaine, and the use of the activated procaine, or salts or solvates thereof, to label amine-containing compounds. In some embodiments, the amine-containing compound is an N-glycan.
ANTIGEN DETECTION USING PHOTOCLEAVABLE LABELS
Granted: June 17, 2021
Application Number:
20210181191
Provided herein are methods of using photocleavable labels for multiplex and serial antigen detection. The methods comprise detecting the presence of photocleavable labels, which are conjugated through functional linkers to antigen-binding complexes, which in turn non-covalently bind to antigens. The presence of a photocleavable label is indicative of the presence of an antigen specifically or selectively bound by an antigen-binding complex. Also provided are apparatuses for using…
AC-Coupled System for Particle Detection
Granted: March 11, 2021
Application Number:
20210074534
A system and method for detecting energetic particles include a detector onto which the particles are impinged. An output signal from the detector, indicative of the energy of the particles, is directed by an AC-coupler to a measurement device to determine particle characteristics such as mass and/or abundance. The detector is selectively couplable to positive or negative bias voltages, and in one embodiment is differentially biased to eliminate ringing due common-mode excitation. The…
USE OF POLYAMINES AS RESOLVING AGENTS FOR CAPILLARY ELECTROPHORESIS OF LABELED GLYCANS USING GELS
Granted: March 4, 2021
Application Number:
20210063347
The present disclosure provides methods of improving the resolution of labeled glycans in capillary electrophoresis techniques using a gel as a sieving matrix, by incorporating polyamines in the gel.
Methods and Kits for Using Blocked 2-AA for Glycan Analysis
Granted: February 18, 2021
Application Number:
20210045981
The present disclosure provides methods for using anthranilic acid (2-AA) blocked by tertbutyloxycarbonyl (Boc) groups in protocols for labeling glycans for analysis, and kits providing 2-AA-Boc and acids for unblocking blocked 2-AA for use in glycan labeling and analysis.
COUPLED-AMPLIFIER MULTI-FREQUENCY CIRCUIT TOPOLOGIES APPLICABLE TO MASS SPECTROMETER RADIO-FREQUENCY DRIVE SYSTEMS
Granted: January 14, 2021
Application Number:
20210013915
A circuit and method for providing high-voltage radio-frequency (RF) energy to an instrument at multiple frequencies includes a plurality of inputs each configured to receive an RF voltage signal oscillating at a corresponding frequency, and a step-up circuit for generating magnified RF voltage signals based on the received RF voltage signals. The step-up circuit includes an LC network operable to isolate the RF voltage signals at the plurality inputs from one another while preserving a…
Use of Tri- and Tetra- Hydroxyl Quaternary Ammonium Compounds As Resolving Agents for Electrophoretic Separations
Granted: November 26, 2020
Application Number:
20200371063
The present disclosure provides methods of improving the resolution of analytes by in electrophoretic separations using a gel by incorporating into the gel an effective amount of one or more tri- or tetra-hydroxyl quaternary ammonium compounds, or a mixture of such compounds.
METHODS, DEVICES, AND KITS TO IMPROVE REDUCTION OR LABELING OF CARBOHYDRATES
Granted: November 5, 2020
Application Number:
20200346214
The present invention provides methods, devices, and kits to improve procedures for reducing carbohydrates, such as glycans released from glycoconjugates, or for labeling carbohydrates by reductive amination.
Multi Frequency LC Resonator Topologies Applicable to Mass Spectrometer Radio Frequency Drive Systems
Granted: October 15, 2020
Application Number:
20200328070
In one embodiment, a power source for providing high-voltage radio-frequency (RF) energy to an instrument such as a mass spectrometer includes an RF power amplifier having an output, an oscillating RF signal generator configured to provide first and second RF signals respectively oscillating at first and second frequencies to the RF power amplifier, and a step-up circuit for magnifying the RF power amplifier output. The step-up circuit includes an LC resonator network tuned to the first…
Mid-Infrared Scanning System for Analyzing Particulates
Granted: October 1, 2020
Application Number:
20200309669
An apparatus and method for analyzing particulates in a sample is disclosed. The method includes placing the sample on a moveable stage in an apparatus having a tunable MIR light scanner and a visible imaging system, the stage moving between the MIR light scanner and the visible imaging system, providing a visible image of the sample, and receiving user input as to a region of the sample that is to be analyzed. The sample is then moved to the MIR light scanner, the MIR light scanner…
SYNTHETIC SILICA AS PACKING MATERIAL IN SUPPORTED LIQUID EXTRACTION
Granted: August 27, 2020
Application Number:
20200269213
In embodiments, a packing material for supported liquid extraction has a sorbent media that includes synthetic silica particles. In embodiments, the synthetic silica particles can have physical properties relating to one or more of particle surface area, shape, size, or porosity. In one embodiment, synthetic silica particles have a surface area less than about 30 m2/g. In another embodiment, the synthetic silica particles have an approximately uniform particle shape. In further examples,…
Method and Apparatus for Characterizing Laser Gain Chips
Granted: August 20, 2020
Application Number:
20200264104
A system adapted for characterizing gain chips and a method for characterizing gain chips are disclosed. The system includes a probe light source that generates an output light signal characterized by a wavelength that can be varied in response to a wavelength control signal and a mounting stage adapted for receiving a gain chip characterized by a waveguide having a reflective face on a first surface of the gain chip and a transparent face on a second surface of the gain chip. The system…
COMPOSITIONS AND METHODS FOR GENOMIC DNA AND GENE EXPRESSION ANALYSIS IN SINGLE CELLS
Granted: July 9, 2020
Application Number:
20200216841
Provided herein are compositions and methods to assess the genomic landscape of fixed cells using light activated oligonucleotides that can be directed to the nucleus, mitochondria, or cytoplasm of fixed cells and that, upon activation, can be extended for in situ copying of nuclear single-stranded DNA (i.e., open chromatin), open mitochondrial DNA, and/or cytoplasmic RNA into barcoded complementary DNA. These methods also provide for gene specific 3D chromatin structural niche analysis.
INDUCTIVELY COUPLED PLASMA MASS SPECTROMETER WITH MASS CORRECTION
Granted: May 7, 2020
Application Number:
20200144046
Systems and methods for controlling mass filtering of polyatomic ions in an ion beam passing through an inductively coupled plasma mass spectrometer (ICP-MS). Polyatomic ion mass data representative of the exact mass of a polyatomic ion having a target isotope is determined. A control signal is generated based on the determined polyatomic ion mass data and output to an ICP-MS to filter based on mass the polyatomic ions in the ion beam traveling through the ICP-MS to an ion detector.
FREQUENCY SWITCHING METHOD APPLICABLE TO MASS SPECTROMETER MULTIPOLE RF DRIVE SYSTEMS
Granted: April 30, 2020
Application Number:
20200136555
In one embodiment, a system includes a mass analyzer, an ion source for providing ions to the mass analyzer, a detector for detecting an output of the mass analyzer, and a frequency-selectable power source. The frequency-selectable power source may include an energy supply configured to provide high-voltage radio-frequency (RF) energy to the mass analyzer at individually selectable first and second frequencies, and a frequency selector for switching between the individually selectable…
Calibration System for Attenuated Total Reflection Spectrometry
Granted: April 2, 2020
Application Number:
20200103278
An ATR scanner and method for calibrating the same are disclosed. The scanner includes an ATR objective having a reflecting face and an optical port adapted to receive a first light beam, and to focus the first light beam to a point, at a location on the reflecting face such that the first light beam is reflected by the reflecting face and no portion of the first light beam strikes the reflecting face at an angle greater than the critical angle. A detector measures an intensity of light…
METHODS, DEVICES, AND KITS TO IMPROVE REDUCTION OR LABELING OF CARBOHYDRATES
Granted: February 6, 2020
Application Number:
20200041522
The present invention provides methods, devices, and kits to improve procedures for reducing carbohydrates, such as glycans released from glycoconjugates, or for labeling carbohydrates by reductive amination.
