Codexis Patent Grants

Engineered proline hydroxylase polypeptides

Granted: October 17, 2017
Patent Number: 9790527
The present disclosure provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents. The present disclosure provides engineered proline hydroxylase biocatalysts, polynucleotides encoding…

Biocatalysts and methods for the synthesis of armodafinil

Granted: September 19, 2017
Patent Number: 9765306
The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (?)-2-[(R)-(diphenylmethyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid,…

Pentose fermentation by a recombinant microorganism

Granted: September 5, 2017
Patent Number: 9752171
The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.

Ketoreductase polypeptides for the production of azetidinone

Granted: August 1, 2017
Patent Number: 9719071
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

Production of fatty alcohols from engineered microorganisms

Granted: July 25, 2017
Patent Number: 9714437
The invention generally relates to the production of a fatty alcohol composition from recombinant microbial cells. The fatty alcohols are produced by expressing a gene encoding a heterologous fatty alcohol forming acyl-CoA reductase (“FAR”); a gene encoding a heterologous thioesterase (“TE”) gene and a gene encoding an acyl-CoA synthetase (“ACS”).

Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system

Granted: July 25, 2017
Patent Number: 9714439
The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.

P450-BM3 variants with improved activity

Granted: July 18, 2017
Patent Number: 9708587
The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.

Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs

Granted: July 18, 2017
Patent Number: 9708588
The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.

Polynucleotides encoding engineered imine reductases

Granted: July 4, 2017
Patent Number: 9695451
The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

P450-BM3 variants with improved activity

Granted: June 20, 2017
Patent Number: 9683220
The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.

Methods, systems, and software for identifying bio-molecules using models of multiplicative form

Granted: June 20, 2017
Patent Number: 9684771
The present invention provides methods for identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. More specifically, some embodiments of the present invention provide methods for building sequence-activity models comprising multiplicative terms and using the models to guide directed evolution. In some embodiments, the sequence-activity models include one or more…

Low-phosphate repressible promoter

Granted: June 6, 2017
Patent Number: 9670493
The present invention provides compositions and methods comprising a low-phosphate repressible promoter. In particular, the present invention provides a low-phosphate repressible promoter from E. coli.

Engineered biocatalysts useful for carbapenem synthesis

Granted: May 30, 2017
Patent Number: 9663771
The present disclosure provides engineered pNB esterase polypeptides useful for the synthesis of the carbapenem antibiotic, imipenem. The disclosure also provides polynucleotides encoding the engineered pNB esterases, host cells capable of expressing the engineered pNB esterases, and methods of using the engineered pNB esterases in the production of imipenem.

Methods, systems, and software for identifying bio-molecules with interacting components

Granted: May 30, 2017
Patent Number: 9665694
The present invention provides methods for rapidly and efficiently searching biologically-related data space. More specifically, the present invention provides methods for identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The present invention also provides methods for modeling sequence-activity relationships, including but not limited to stepwise addition or…

Engineered ketoreductase polypeptides

Granted: May 23, 2017
Patent Number: 9657320
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the…

Production of fatty alcohols from engineered microorganisms

Granted: May 16, 2017
Patent Number: 9650655
Recombinant microorganisms are provided which have been engineered to produce fatty alcohols. Also provided are recombinant microorganisms which comprise a heterologous polynucleotide encoding a fatty alcohol reductase enzyme and an introduced polynucleotide encoding a ?-ketoacyl acyl carrier protein synthase.

Biocatalysts for ezetimibe synthesis

Granted: May 9, 2017
Patent Number: 9644189
The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.

Synthesis of prazole compounds

Granted: April 25, 2017
Patent Number: 9631181
The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.

Biocatalysts and methods for the synthesis of substituted lactams

Granted: April 18, 2017
Patent Number: 9624478
The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.

Enone reductases

Granted: April 11, 2017
Patent Number: 9617568
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.