Biocatalysts and methods for hydroxylation of chemical compounds
Granted: March 11, 2025
Patent Number:
12247234
The present disclosure provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.
Transaminase reactions
Granted: March 11, 2025
Patent Number:
12247233
The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.
Biocatalysts for the preparation of hydroxy substituted carbamates
Granted: March 4, 2025
Patent Number:
12241093
The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
Granted: February 4, 2025
Patent Number:
12215368
The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
Engineered glucose dehydrogenases and methods for the reductive amination of ketone and amine compounds
Granted: February 4, 2025
Patent Number:
12215359
The present application provides engineered glucose dehydrogenase polypeptides having imine reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
Engineered cyclic GMP-AMP synthase (cGAS) variant enzymes
Granted: December 31, 2024
Patent Number:
12180483
Cyclic GMP-AMP synthase (cGAS) enzymes have been engineered to produce polypeptides having increased cGAS activity in the cyclization of modified nucleoside triphosphates, including thiolated or fluorinated nucleoside triphosphates, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing cGAS enzymes, compositions comprising the cGAS enzymes, and methods of using the engineered cGAS enzymes are…
Biocatalysts and methods for the synthesis of substituted lactams
Granted: December 10, 2024
Patent Number:
12163162
The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.
Engineered DNA polymerase variants
Granted: October 29, 2024
Patent Number:
12129495
The present disclosure relates to engineered DNA polymerase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered DNA polymerase polypeptides. The present disclosure also provides methods of using the engineered DNA polymerase polypeptides or compositions thereof for diagnostic and other purposes.
T7 RNA polymerase variants
Granted: October 29, 2024
Patent Number:
12129494
The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5?)ppp(5?)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.
Engineered pantothenate kinase variant enzymes
Granted: October 8, 2024
Patent Number:
12110513
The present invention provides engineered pantothenate kinase (PanK) enzymes, polypeptides having PanK activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PanK enzymes are also provided. The present invention further provides compositions comprising the PanK enzymes and methods of using the engineered PanK enzymes. The present invention finds particular use in the production of…
Biocatalysts for ezetimibe synthesis
Granted: October 8, 2024
Patent Number:
12110512
The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Engineered purine nucleoside phosphorylase variant enzymes
Granted: October 8, 2024
Patent Number:
12110493
The present invention provides engineered purine nucleoside phosphorylase (PNP) enzymes, polypeptides having PNP activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PNP enzymes are also provided. The present invention further provides compositions comprising the PNP enzymes and methods of using the engineered PNP enzymes. The present invention finds particular use in the…
Penicillin-G acylases
Granted: September 10, 2024
Patent Number:
12084697
The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, and methods of using the engineered PGA enzymes.
Engineered amylase variants
Granted: August 27, 2024
Patent Number:
12071642
The present invention provides engineered amylase polypeptides and compositions thereof. The engineered amylase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH<7) conditions. The invention also relates to the use of the compositions comprising the engineered amylase polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides…
Lov-D acyltransferase mediated acylation
Granted: August 13, 2024
Patent Number:
12060592
Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.
Ketoreductase polypeptides for the production of azetidinone
Granted: July 30, 2024
Patent Number:
12049649
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Ketoreductase polypeptides for the preparation of phenylephrine
Granted: July 9, 2024
Patent Number:
12031159
The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine.
Engineered DNA polymerase variants
Granted: June 25, 2024
Patent Number:
12018295
The present invention provides engineered DNA polymerase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered DNA polymerase polypeptides. The invention also provides methods for use of the compositions comprising the engineered DNA polymerase polypeptides for diagnostic and other purposes.
Engineered galactose oxidase variant enzymes
Granted: June 18, 2024
Patent Number:
12012618
The present invention provides engineered galactose oxidase (GOase) enzymes, polypeptides having GOase activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing GOase enzymes are also provided. The present invention further provides compositions comprising the GOase enzymes and methods of using the engineered GOase enzymes. The present invention finds particular use in the production of…
Peroxidase activity towards 10-acetyl-3,7-dihydroxyphenoxazine
Granted: June 18, 2024
Patent Number:
12012620
The present invention provides engineered peroxidase enzymes, polypeptides having peroxidase activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing peroxidase enzymes are also provided. The present invention further provides compositions comprising the peroxidase enzymes and methods of using the engineered peroxidase enzymes. The present invention finds particular use in the…
