Methods for identifying and producing polypeptides
Granted: June 7, 2011
Patent Number:
7957912
In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided. One disclosed method involves identifying and producing a polypeptide. The method may involve (a) receiving data identifying sequences of two or more parental polypeptides or parental nucleic acids that encode the polypeptides; (b) selecting one or more cross-over sites on the sequences thereby defining one or more…
Alcohol dehydrogenase for the stereoselective production of hydroxy compounds
Granted: May 17, 2011
Patent Number:
7943356
The invention relates to a DNA sequence encoding an NADP-dependent alcohol dehydrogenase, to a vector containing at least one copy of the DNA sequence and to prokaryotic or eukaryotic host cells that are transformed or transfected with the DNA sequence. The invention also relates to the NADP-dependent alcohol dehydrogenase, to a method for the production and the use of the alcohol dehydrogenase and to a method for the stereoselective production of secondary alcohols.
Glucose dehydrogenase polypeptides and related polynucleotides
Granted: May 10, 2011
Patent Number:
7939309
The present invention is directed to glucose dehydrogenase (GDH) polypeptides that have enhanced GDH activity and/or thermostability relative to the backbone wild-type glucose dehydrogenase polypeptide. In addition, the present invention is directed to a polynucleotide that encodes for the GDH polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells…
Methods for identifying sets of oligonucleotides for use in an in vitro recombination procedures
Granted: March 8, 2011
Patent Number:
7904249
In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators are used to identify sets of oligonucleotides for in vitro recombination. In certain embodiments, the methods involve inputting amino acid sequence character strings into a digital system and back-translating the amino acid character strings into a plurality of nucleic acid character strings. The nucleic acid character strings are then processed to define and select at least one…
Ketoreductase polypeptides for the production of azetidinone
Granted: February 8, 2011
Patent Number:
7883879
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Ketoreductase enzymes and uses thereof
Granted: February 1, 2011
Patent Number:
7879585
The present disclosure provides ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, method of using the engineered ketoreductase enzymes to synthesize a variety of chirally pure compounds, and the chirally pure compounds prepared therewith.
Method and system using systematically varied data libraries
Granted: January 18, 2011
Patent Number:
7873477
Methods and systems for providing biological results in the form of systematically varied libraries of sequences or as data representing sequences or physical preparations of systematically varied libraries and/or selections from systematically varied libraries.
Methods of populating data structures for use in evolutionary simulations
Granted: January 18, 2011
Patent Number:
7873499
In particular, this invention provides novel methods of populating data structures for use in evolutionary modeling. In particular, this invention provides methods of populating a data structure with a plurality of character strings. The methods involve encoding two or more a biological molecules into character strings to provide a collection of two or more different initial character strings; selecting at least two substrings from the pool of character strings; concatenating the…
Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
Granted: January 11, 2011
Patent Number:
7868138
A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
Method for making polynucleotides having desired characteristics
Granted: December 14, 2010
Patent Number:
7853410
In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided.
Ketoreductase polypeptides and related polynucleotides
Granted: November 16, 2010
Patent Number:
7833767
The present invention is directed to variant polypeptides having enhanced ketoreductase activity and/or thermostability for use in the stereospecific reduction of ketones. In addition, the present invention is directed to polynucleotides that encode the ketoreductase polypeptides, including codon optimized versions of the polynucleotides which provide for enhanced expression in host cells. In another aspect, the present invention is directed to nucleotide constructs, vectors and host…
Halohydrin dehalogenases and related polynucleotides
Granted: November 2, 2010
Patent Number:
7824898
The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.
Ketoreductases and uses thereof
Granted: October 26, 2010
Patent Number:
7820421
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Glucose dehydrogenase polypeptides and related polynucleotides
Granted: October 19, 2010
Patent Number:
7816111
The present invention is directed to glucose dehydrogenase (GDH) polypeptides that have enhanced GDH activity and/or thermostability relative to the backbone wild-type glucose dehydrogenase polypeptide. In addition, the present invention is directed to a polynucleotide that encodes for the GDH polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells…
Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives
Granted: October 5, 2010
Patent Number:
7807423
The present invention provides methods and compositions for preparing 4-substituted 3-hydroxybutyric acid derivatives by halohydrin dehalogenase-catalyzed conversion of 4-halo-3-hydroxybutyric acid derivatives. The present invention further provides methods and compositions for preparing 4-halo-3-hydroxybutyric acid derivatives by ketoreductase-catalyzed conversion of 4-halo-3-ketobutyric acid derivatives The present invention also provides methods and compositions for preparing vicinal…
Alanine 2,3-aminomutases and related polynucleotides
Granted: September 7, 2010
Patent Number:
7790432
The present invention is directed to polypeptides that have enhanced alanine 2,3-aminomutase (AAM) activity and/or thermostability relative to the wild-type enzymes that have incidental AAM activity as a result of cross reactivity with alanine. In addition, the present invention is directed to a polynucleotides that encodes for the AAM polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides…
Ribulose, 1,5-bisphosphate carboxylase/oxygenase polypeptides and related polynucleotides
Granted: June 29, 2010
Patent Number:
7745186
The present invention relates to novel ribulose-1,5-bisphosphate carboxylase/oxygenase polypeptides and the polynucleotides that encode them. The invention also provides related host cells and methods.
Enzymatic conversion of epoxides
Granted: April 13, 2010
Patent Number:
7695942
The invention relates to a process for converting an epoxide to an alcohol. The process according to the invention is enzymatically catalyzed and highly enantioselective and regiospecific.
Method for reductive amination of a ketone using a mutated enzyme
Granted: January 5, 2010
Patent Number:
7642073
Methods for chemically transforming compounds using a mutated enzyme are provided, and more particularly a method for the production of an amine from a target ketone. The methods comprise creating a mutated enzyme that catalyzes the reductive amination of the target ketone under conditions sufficient to permit the formation of the desired amine to thereby produce the amine.
Ketoreductase polypeptides and related polynucleotides
Granted: December 8, 2009
Patent Number:
7629157
The present invention is directed to variant polypeptides having enhanced ketoreductase activity and/or thermostability for use in the stereospecific reduction of ketones. In addition, the present invention is directed to polynucleotides that encode the ketoreductase polypeptides, including codon optimized versions of the polynucleotides which provide for enhanced expression in host cells. In another aspect, the present invention is directed to nucleotide constructs, vectors and host…
