Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system
Granted: March 31, 2020
Patent Number:
10604781
The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.
Engineered transaminase polypeptides for industrial biocatalysis
Granted: March 31, 2020
Patent Number:
10604744
The present disclosure provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The disclosure also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.
Ketoreductase polypeptides for the production of azetidinone
Granted: March 24, 2020
Patent Number:
10597641
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Variant LovD polypeptides and their uses
Granted: March 17, 2020
Patent Number:
10590445
The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound.
Ketoreductase polypeptides for the preparation of phenylephrine
Granted: March 17, 2020
Patent Number:
10590396
The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine.
Polynucleotides encoding cyclohexanone monooxygenases
Granted: February 11, 2020
Patent Number:
10557126
The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (?)-2-[(R)-(diphenylmethyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid,…
Transaminase polypeptides
Granted: February 4, 2020
Patent Number:
10550370
The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.
Biocatalysts for ezetimibe synthesis
Granted: January 28, 2020
Patent Number:
10544400
The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs
Granted: January 28, 2020
Patent Number:
10544402
The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.
Ketoreductase polypeptides
Granted: January 28, 2020
Patent Number:
10544401
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the…
Engineered tyrosine ammonia lyase
Granted: January 14, 2020
Patent Number:
10533168
The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.
Engineered biocatalysts and methods for synthesizing chiral amines
Granted: January 7, 2020
Patent Number:
10526587
The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.
Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
Granted: December 3, 2019
Patent Number:
10494656
The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
Engineered phenylalanine ammonia lyase polypeptides
Granted: December 3, 2019
Patent Number:
10494623
The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.
Enone reductases
Granted: December 3, 2019
Patent Number:
10494615
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
Engineered phenylalanine ammonia lyase polypeptides
Granted: November 26, 2019
Patent Number:
10487319
The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.
Methods, systems, and software for identifying functional bio-molecules
Granted: October 22, 2019
Patent Number:
10453554
The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention…
P450-BM3 variants with improved activity
Granted: October 22, 2019
Patent Number:
10450550
The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.
Engineered biocatalysts and methods for synthesizing chiral amines
Granted: October 8, 2019
Patent Number:
10435674
The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.
Lov-D acyltransferase mediated acylation
Granted: October 1, 2019
Patent Number:
10428360
Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.
