Liquid chromatography columns with structured walls
Granted: September 11, 2018
Patent Number:
10073068
A liquid chromatography (LC) column includes a wall having a length along a central axis from the inlet end to the outlet end, the wall enclosing a column interior and having a column radius relative to the central axis, the wall comprising a structured portion configured such that the column radius varies along the length; and a plurality of particles packed in the column interior, wherein at least some of the particles are in contact with the structured portion.
Gas chromatography (GC) column heater
Granted: September 4, 2018
Patent Number:
10067101
A gas chromatography (GC) column heating apparatus is described. The GC column heating apparatus includes a first substrate; a heating element disposed over the first substrate; and a second substrate. A gas chromatography (GC) column heating and cooling apparatus is also described. The GC column cooling and heating apparatus includes a housing configured to receive a heating apparatus, the heating apparatus comprising a first side and a second side; a first thermal insulation layer…
Automatic determination of demultiplexing matrix for ion mobility spectrometry and mass spectrometry
Granted: July 31, 2018
Patent Number:
10037873
Multiplexed ion mobility spectrometry (IMS), mass spectrometry (MS) such as time-of-flight mass spectrometry (TOFMS), or hybrid IM-MS is carried out on a sample, and the resulting measurement data are deconvoluted. A pulse sequence controlling ion pulsing is utilized in conjunction with the multiplexing. The pulse sequence may be modified based on the raw measurement data acquired. A demultiplexing matrix based on the modified pulse sequence is utilized to improve deconvolution.
Chemical arrays and methods of using the same
Granted: July 10, 2018
Patent Number:
10017879
Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties.…
Detection of genomic rearrangements by sequence capture
Granted: July 10, 2018
Patent Number:
10017820
Provided herein is a method of sample analysis. In some embodiments, the method comprises hybridizing fragmented genomic DNA from a test genome with a population of first oligonucleotides of the formula V1-B-V2 in the presence of one or more second oligonucleotides; contacting the product with ligase to join the ends of the fragmented genomic DNA that are hybridized to V1 and V2 to the one or more second oligonucleotides; and subjecting the product to polymerase chain reaction conditions…
Chromatograph
Granted: July 10, 2018
Patent Number:
D822848
Droplet actuation enhancement using oscillatory sliding motion between substrates in microfluidic devices
Granted: July 3, 2018
Patent Number:
10010884
A droplet-based microfluidic device having a first confining plate, a second confining plate, and an actuator. Each confining plate includes a respective substrate and hydrophobic layer having a planar major surface. The first confining plate additionally includes a common electrode between its hydrophobic layer and substrate. The second confining plate includes an electrode array between its hydrophobic layer and substrate. The confining plates are disposed opposite one another with…
Method transfer between fluidic devices considering deviations from ideal behavior
Granted: July 3, 2018
Patent Number:
10012624
An apparatus for deriving an operation mode from a first fluidic device to a second fluidic device, wherein the first fluidic device has a first target operation mode representing a desired behavior of the first fluidic device and has a first real operation mode representing the actual behavior of the first fluidic device, wherein the second fluidic device has a second target operation mode representing a desired behavior of the second fluidic device and has a second real operation mode…
Sealed fluidic component comprising a composite material of different paek materials
Granted: July 3, 2018
Patent Number:
10012621
A sealed fluidic component for use in a fluidic flow path is made by providing a composite material comprising a first material and a second material, wherein the first material and the second material are different PAEK materials with the first material having a lower melting point than the second material. The composite material is heated to provide a sealing by the first material.
Method for metabolomic sample preparation based on ionic liquid dispersive liquid-liquid microextraction
Granted: July 3, 2018
Patent Number:
10012574
Provided herein is a method comprising one or more of the following steps: (a) lysing cells of a biological sample and contacting the biological sample with an amount of ionic liquid sufficient to denature intracellular metabolic enzymes in the biological sample to produce a contacted cellular sample; (b) mixing the contacted cellular sample with an organic solvent to produce an ionic liquid-organic solvent composition; (c) mixing the contacted cellular sample with the organic solvent to…
Full field visual-mid-infrared imaging system
Granted: June 19, 2018
Patent Number:
10003754
An apparatus and method for generating images of specimens is disclosed. The apparatus includes an imaging system, controller, and user interface. The imaging system generates a plurality of component images of a specimen, each component image corresponding to a different viewing condition. Each image is represented by an intensity as a function of location on the specimen. The controller stores the component images and generates a compound image from a plurality of the component images.…
Vibration damper for vacuum pumps
Granted: June 12, 2018
Patent Number:
9995421
In a vibration damper of a turbomolecular vacuum pump, a vacuum tightness function and a vibration damping function are both performed by one ore more toroidal rings made of an elastic material. The elastic rings assume a resting configuration while the vacuum pump is not operating and the damper is at atmospheric pressure, and they assume a compressed configuration while the vacuum pump is operating and the damper is under vacuum conditions. In the compressed configuration, the elastic…
Noise reduction for pulsed lasers using clustering
Granted: June 5, 2018
Patent Number:
9989414
An optical measurement method in which a series of light pulses are generated using a pulsed laser having a set of different mode hop sequences (e.g., an external-cavity quantum cascade laser (EC-QCL)), the light pulses are detected with the detector to generate a respective pulse data set for each of the light pulses, and the pulse data sets are sorted into classes based on correlation coefficients. Sorting the pulse data sets into classes allows the pulse data sets originating from…
Pairing code directed assembly
Granted: May 22, 2018
Patent Number:
9976162
Provided herein is a method comprising: producing a first complex by annealing: a first nucleic acid comprising, in order, a first unique sequence, a first central sequence and a second unique sequence; and a second nucleic acid comprising, in order, said second unique sequence, a second central sequence and a third unique sequence; wherein the first, second and third unique sequences do not hybridize with each other; subjecting said first complex to multiple rounds of primer extension…
Pulsed ion guides for mass spectrometers and related methods
Granted: May 15, 2018
Patent Number:
9972480
An ion guide generates a radio frequency (RF) field to radially confine ions to an ion beam along a guide axis as the ions are transmitted through the ion guide. The effective potential of the RF field includes an alternating series of barriers and wells. Ions may be trapped in individual wells in mass-dependent order, with larger masses trapped closer to a guide exit than smaller masses. The RF field may be scanned so as to release the ions from the ion guide in mass-dependent order,…
Coated capillary with remelted coating for front sided sealing
Granted: May 8, 2018
Patent Number:
9962695
A method of manufacturing a sealing fluidic component based on a capillary enclosing a fluid conduit and having an exterior surface being at least partially coated with a coating of a meltable material, wherein the method comprises melting the meltable material of the coating at least at an end portion of the capillary, and resolidifying the melted material to thereby form, at the end portion, a sealing integral with the coating and constituted at least partially by the meltable…
Fluorous affinity extraction for ionic liquid-based sample preparation
Granted: May 1, 2018
Patent Number:
9958363
A method for removing an ionic liquid from an aqueous sample is provided. In some embodiments, the method includes: (a) combining an aqueous sample including an ionic liquid with an ion exchanger composition including an ion exchanger counterion to produce a solution including a fluorous salt of the ionic liquid, where at least one of the ionic liquid and the ion exchanger counterion is fluorinated; (b) contacting the solution with a fluorous affinity material, thereby removing fluorous…
Mixer bypass sample injection for liquid chromatography
Granted: April 17, 2018
Patent Number:
9945820
In a liquid chromatography system, a sample is injected into a column by flowing a solvent mixture from a mixer into the column along a solvent mixture flow path; and injecting a sample into the solvent mixture flow path downstream of the mixer. In another liquid chromatography system, a sample is injected into a column by loading an isolator fluid into a sample loop, loading a sample into the sample loop, and flowing the sample into the column as a plug in front of the isolator fluid.
Apparatus and method for introducing sample into a separation unit of a chromatography system without disrupting a mobile phase
Granted: April 17, 2018
Patent Number:
9945762
A method and a system for introducing a sample into a mobile phase of a chromatography system is provided. The method includes initially directing the mobile phase directly into a separation unit of the chromatography system, bypassing a sample loop, the mobile phase including a combined solvent, metered from a pressurized first solvent and a second solvent; loading the sample into the sample loop, while the mobile phase continues to be directed directly into the separation unit;…
Ferrule
Granted: April 10, 2018
Patent Number:
D815256
