Sample sprayer with adjustable conduit and related methods
Granted: June 6, 2017
Patent Number:
9673032
A sample sprayer includes a first conduit for conducting a liquid sample, a second conduit surrounding the first conduit to define an annular passage for conducting a gas, a sprayer tip in which a fluid interaction region receives the liquid sample and the gas. The sprayer tip is configured to produce a sample spray by contact between the liquid sample and the gas in the fluid interaction region and emit the sample spray from the orifice. An adjustable positioning device is configured to…
Fluidic chip with displacable patterned layer for detecting fluid pressure
Granted: June 6, 2017
Patent Number:
9671375
A fluidic chip device configured for processing a fluid, wherein the fluidic chip device comprises a plurality of layers laminated to one another, wherein at least a part of the layers comprises a patterned section of an alternating sequence of bars and fluidic channels for conducting the fluid under pressure, the patterned section being configured for being displaceable in response to the pressure, and a pressure detector responding to the displacement of the patterned section by…
Rotary vacuum pump
Granted: June 6, 2017
Patent Number:
9670931
A rotary vacuum pump includes a first set of pumping stages having rotor discs with a first diameter and a second set of pumping stages having rotor discs with a second different diameter and further comprising an intermediate pumping stage that is particularly adapted for matching the change in the diameter of the pump rotor discs. According to the invention, the intermediate pumping stage comprises a rotor disc having an outer portion in the radial direction that is axially tapered…
Microfluidic contaminant trap for trapping contaminants in gas chromatography
Granted: May 30, 2017
Patent Number:
9664598
Microfluidic contaminant traps of certain representative embodiments illustratively comprise: an inlet configured to connect directly or indirectly to a sample inlet of a gas chromatography (GC) system; an outlet configured to connect directly to an inlet of a GC column or indirectly to the GC column via another fluidic component; an interlayer comprising a channel; an upper layer disposed over and bonded to the interlayer; and a coating disposed over the channel. The coating reduces…
Method for amplification of target nucleic acid
Granted: May 23, 2017
Patent Number:
9657339
A method for amplifying a target nucleic acid is disclosed, which includes: (a) fragmenting a nucleic acid sample to create a target fragment comprising a target nucleic acid and two probe-complementary portions; (b) contacting said fragmented nucleic acid sample with a probe comprising two target fragment-complementary portions complementary to the probe-complementary portions of the target fragment; (c) rendering the fragmented nucleic acid sample single-stranded; (d) allowing the…
Light source with controllable linear polarization
Granted: May 16, 2017
Patent Number:
9651426
A light source having first and second wire-grid polarizers and a laser that emits a beam of linearly polarized light that is characterized by a propagation direction is disclosed. The first wire-grid polarization filter is characterized by a first linear polarization pass direction and a first actuator for causing the first linear polarization pass direction to rotate relative to the beam of linearly polarized light. The second wire-grid polarization filter is characterized by a second…
Immobilized transposase complexes for DNA fragmentation and tagging
Granted: May 9, 2017
Patent Number:
9644199
The present invention provides a simple and rapid method for preparing purified transposase complexes that are highly suited for fragmenting DNA. The method includes forming transposase complexes with oligonucleotide adapters in cell lysate, then purifying the complexes from the other substance in the cell lysate. Purification is accomplished using a specific binding pair, in which one member of the pair is bound to an oligonucleotide adapter of the complex and the other member of the…
GC column connection with a planar connection to mating devices
Granted: May 2, 2017
Patent Number:
9638676
A representative embodiment is directed to a fitting for fluidically coupling a GC column to another structure. The fitting comprises: a first end configured to receive a ferrule having a tubular element disposed therein, the tubular element being oriented in a first direction; and a second end fluidically connected to the first end and having an opening to provide a fluid from the tubular element in a second direction that is different from the first direction. The second end comprises…
Chromatograph
Granted: April 25, 2017
Patent Number:
D785198
Determination of a fluid loss of a piston pump based on evacuation time variation
Granted: April 25, 2017
Patent Number:
9631618
A device for determining a leakage of fluid in a piston pump, wherein the piston pump comprises a piston arranged in such a manner that it can reciprocate in a piston chamber for delivering fluid, wherein the device comprises a control unit for controlling the piston in such a manner that the piston executes two piston chamber evacuation processes with different evacuation times, in each case for at least partial evacuating of fluid located in the piston chamber, and a determination unit…
Energy resolved time-of-flight mass spectrometry
Granted: April 18, 2017
Patent Number:
9627190
A time-of-flight mass spectrometer (TOF-MS) utilizes an ion dispersion device and a position-sensitive ion detector or an energy-sensitive ion detector to enable measurement of time of flight and kinetic energy of ions arriving at the detector. The measurements may be utilized to improve accuracy in calculating ion masses.
Magnifying assembly for an infrared microscope
Granted: April 18, 2017
Patent Number:
9625695
An infrared microscope includes a sample stage configured to support a sample; an objective configured to focus radiation emanated from the sample to an intermediate image plane between an objective and an infrared detector; and a magnifying assembly including a first set of reflective elements provided in a fixed position and a second set of reflective elements.
Hydrolytically stable ion-exchange stationary phases and uses thereof
Granted: April 4, 2017
Patent Number:
9610576
The invention provides novel ion-exchange media and related methods for their preparation and use. Ion-exchange stationary phases according to the invention are suitable for chromatographic separation of a variety of biomolecules. Distinguishing characteristics of ion-exchange media according to this invention includes, for example, their ability to separate variants of monoclonal antibodies via cation-exchange liquid chromatography using porous substrates with particle sizes <5 ?m.…
Vacuum scroll pump having pressure-balanced orbiting plate scroll
Granted: March 28, 2017
Patent Number:
9605674
A vacuum scroll pump has a frame, a stationary plate scroll fixed to the frame, an orbiting plate scroll, an eccentric drive mechanism for driving the orbiting plate scroll, and counterbalancing features by which axial loads produced on the eccentric drive mechanism are offset. Scroll blades of the stationary and orbiting plate scrolls are nested to define pockets which constitute a compression stage between opposing front sides of plates of the stationary and orbiting plate scrolls. The…
Alternating current (AC) coupler for wideband AC signals and related methods
Granted: March 7, 2017
Patent Number:
9590583
An AC coupler for transmitting high-frequency components of a wideband signal includes a signal conductor and a shielding structure arranged as a transmission line. The signal conductor includes a conductive element and a capacitor configured to block direct current (DC) components of the wideband signal while transmitting high-frequency alternating current (AC) components of the wideband signal. The shielding structure is configured for conducting at least the AC components of the…
Plasma cleaning for mass spectrometers
Granted: March 7, 2017
Patent Number:
9589775
A mass spectrometry (MS) system may be cleaned by generating plasma and contacting an internal surface of the system to be cleaned with the plasma. The system may be switched between operating in an analytical mode and in a cleaning mode. In the analytical mode a sample is analyzed, and plasma may or may not be actively generated. In the cleaning mode the plasma is actively generated, and the sample may or may not be analyzed.
Fast hybridization for next generation sequencing target enrichment
Granted: March 7, 2017
Patent Number:
9587268
The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.
Synthesis of long fish probes
Granted: February 28, 2017
Patent Number:
9580746
A method comprising: synthesizing a set of overlapping oligonucleotides that comprises probe sequences that hybridize to unique sequences in a chromosome, assembling the overlapping oligonucleotides in a way that produces one or more double stranded polynucleotides that each comprises multiple probe sequences, labeling the one or more double stranded polynucleotides to produce one or more labeled probes, and hybridizing the labeled probes to an intact chromosome, in situ, is provided.
Isolation of RNA-protein complexes using cross-linking reagents and oligonucleotides
Granted: February 21, 2017
Patent Number:
9574236
Provided herein is a method of sample analysis. In certain embodiments, the method comprises: a) cross-linking protein of a cell using a first compound to produce a first cross-linked product comprising cross-linked protein, and RNA; b) contacting the first cross-linked product and a second compound under conditions by which an oligonucleotide portion of the second compound hybridizes to the RNA; c) activating a reaction the first and second compound, thereby covalently crosslinking the…
Data processing for multiplexed spectrometry
Granted: February 21, 2017
Patent Number:
9576778
Multiplexed spectrometry, such as multiplexed ion mobility spectrometry (IMS), time-of-flight mass spectrometry (TOFMS), or hybrid IM-TOFMS, is carried out on a sample, and the resulting measurement data are deconvoluted. Noise may be removed from the measurement data prior to deconvolution. Alternatively or additionally, noise may be removed from the deconvoluted data.
