Process of producing B-lactam antibiotics applying microorganisms with increased ligase activity
Granted: August 24, 1999
Patent Number:
5942411
The present invention relates to the biosynthesis of .beta.-lactam antibiotics. More specifically, the invention relates to processes of producing .beta.-lactam antibiotics in vivo and in vitro. Also contemplated is a novel enzyme capable of catalyzing certain steps involved in .beta.-lactam biosynthesis. Further, the invention relates to a DNA construct encoding the novel enzyme, a recombinant vector or transformation vehicle comprising the DNA construct, and finally a cell comprising…
Pectinesterase in the treatment of fruit and vegetables
Granted: August 17, 1999
Patent Number:
5939110
The present invention discusses the use of pectinesterase to demethoxylate high-methoxylated pectins. The pectinesterase makes possible the preparation of food containing fruit or vegetables in a more efficient way. Specifically, fruit and vegetable jams, jellies, compotes and soups are prepared without or with greatly reduced sugar and pectin addition. The present invention also discusses a process for producing apple sauce comprising the use of pectinesterase.
Mutated .beta.-lactam acylase genes
Granted: August 10, 1999
Patent Number:
5935831
New mutant .beta.-lactam acylases are provided exhibiting altered substrate specificities. These .beta.-lactam acylases are obtained by expression of a gene encoding said .beta.-lactam acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type .beta.-lactam acylase.
Direct selection of transformants on acetate-containing media
Granted: July 20, 1999
Patent Number:
5925515
A transformant selection system has been developed, particularly for a .beta.-lactam producing strain, more particularly for P. chrysogenum, by the complementation of a mutation of said .beta.-lactam producing strain by a homologous selection marker without interfering with .beta.-lactam biosynthesis. Particularly, in applying said transformant selection system a positive selection agent, for instance fluoroacetate is used for the isolation of fac mutants of said strain, particularly of…
De-esterification process
Granted: July 13, 1999
Patent Number:
5922861
An efficient process for de-esterification has been provided for by application of special tetrahalogenides. By applying this process a new compound, viz. cefesone, and especially the E-isomer thereof, has been prepared.
Phytosphingosine-based ceramide I analogs
Granted: July 6, 1999
Patent Number:
5919960
The present invention describes phytospringosine-based analogs of ceramides I, as well as cosmetics and pharmaceutical compositions containing these novel compounds and their use.
Process for increasing the volume of a baked product
Granted: June 29, 1999
Patent Number:
5916607
The present invention relates to use of cyclodextrin glucanotransferase in the preparation of doughs for baked products, e.g. bread doughs. In particular, the invention provides a process for the preparation of a dough for a baked product which includes incorporating into the dough cyclodextrin glucanotransferase in an amount to increase the volume of the baked product.
Baker's yeast and a method producing it
Granted: June 29, 1999
Patent Number:
5916609
A process is disclosed for the production of baker's yeast which comprises the fermentation of a baker's yeast strain using a non-molasses carbon source until a fermentation broth having at least 10%, preferably at least 13%, more preferably at least 16% of dry solids content is formed, which can be used directly as a cream yeast without concentration.
Production of aminopeptidases form aspergillus niger
Granted: June 22, 1999
Patent Number:
5914259
Aminopeptidases originating from Aspergilli niger are disclosed, which can be produced in a fermentation broth or a liquid concentrate thereof substantially free of endoprotease. Such aminopeptidases may be advantageously employed in the food industry, e.g. in the preparation of bread doughs or cheese.
Microbial strains producing sphingolipid bases
Granted: June 8, 1999
Patent Number:
5910425
Microbial strains capable of producing enhanced levels of sphingosine, dihydrosphingosine, phytosphingosine and/or derivatives thereof are disclosed. Additionally, there are disclosed methods based on mutagenesis, or other selection techniques, whereby such strains can be produced. As a preferred example thereof, mutant strains of Pichia are provided that are capable of producing about 50% more of such compounds than wild type strains.
Enzymatic production of gluconic acid or its salts
Granted: April 27, 1999
Patent Number:
5897995
An enzymatic process for the conversion of glucose into gluconic acid, uses concentrated glucose solutions. The process employs a combination of glucose oxidase and catalase enzymes which may be obtained from an Aspergilltus niger strain and has a high ratio of catalase;glucose oxidase activity. The enzymatic process requires less time than conventional fermentation processes, the yield of the conversion is close to 100% and the obtained gluconic acid/gluconate solutions do not contain…
Method for influencing .beta.-lactam antibiotic production and for isolation of large quantities of ACV synthetase
Granted: March 16, 1999
Patent Number:
5882879
Novel methods and compositions are provided for the enhanced production of .beta.-lactam antibiotics. The process is exemplified by the production of penicillin. In addition, the P. chrysogenum and A. chrysogenum .delta.-(L-.alpha.-aminoadipyl)-L-cysteinyl-D-valine synthetase genes have been isolated and sequenced. Also methods are provided for the production of .delta.-(L-.alpha.-aminoadipyl)-L-cysteinyl-D-valine synthetase using recombined DNA techniques.
Strains of phaffia rhodozyma containing high levels of astaxanthin and low levels of 3-hydroxy-3', 4'-didehydro-.beta., .psi.-caroten-4-one (HDCO)
Granted: March 9, 1999
Patent Number:
5879927
The present invention provides Phaffia rhodozyma strains having increased levels of astaxantin and a low percentage of 3-hydroxy-3',4'-didehydro-.beta.,.psi.-caroten-4-one (HDCO). Such strains are obtained by a combination of mutagenesis and selection. The invention further provides a method for obtaining such strains. The invention also provides astaxanthin obtained from a mutagenized strain of Phaffia rhodozyma characterized in that it contains a decreased relative amount of HDCO.
Selection marker gene free recombinant strains: method for obtaining them and the use of these strains
Granted: March 2, 1999
Patent Number:
5876988
The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired…
Cloning and expression of microbial phytase
Granted: January 26, 1999
Patent Number:
5863533
A nucleotide sequence encoding phytase has been isolated and cloned. The coding sequence has been inserted into an expression construct which in turn has been inserted into a vector capable of transforming a microbial expression host. The transformed microbial hosts may be used to economically produce phytase on an industrial scale. The phytase produced via the present invention may be used in a variety of processes requiring the conversion of phytate to inositol and inorganic phosphate.
Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin
Granted: January 26, 1999
Patent Number:
5863783
The present invention provides the cloning and expression of purified and isolated DNA molecules, obtainable from fungi, which encode enzymes having arabinan-degrading activity. The present invention also provides DNA constructs containing these DNA molecules and methods for their enhanced expression in selected microbial host cells. The present invention further provides methods for the use of the thus-produced arabinan-degrading enzymes.
electronic equipment enclosure
Granted: January 12, 1999
Patent Number:
D404017
Yeast strains for saccharide fermentation
Granted: January 12, 1999
Patent Number:
5858764
New yeast strains providing for an enhanced rate of the fermentation of sugars, and a process to obtain such yeasts and the use of these yeasts. Yeasts capable of improved fermentation of sugars, a process to obtain these yeasts and the use of these yeasts are provided. The yeasts show higher rates of metabolism resulting in for example higher carbon dioxide and ethanol production in media containing sugars, such as maltose, as main carbon and energy source. The fermentation rate of…
Arabinoxylan degrading enzymes
Granted: December 15, 1998
Patent Number:
5849559
Methods and expression constructs are provided for the cloning and overexpression of an arabinoxylan degrading enzyme of fungal origin in a selected microbial host cell. The enzyme is shown to be active in the degradation of water-insoluble solids obtained from maize. The enzyme can be used in the preparation of animal feed compositions, human food or in industrial processes.
Process for the purification of serum albumin
Granted: December 15, 1998
Patent Number:
5849874
Recombinantly produced serum albumin is purified in a series of steps, optionally by incubation with an anion-exchange adsorbent, followed by affinity chromatography employing a hydrophobic solid phase and using a water-soluble lipid anion as desorbens in the aqueous phase. This immobile phase comprises a carrier coupled to a 2-mercapto or 2-hydroxy alkanoic acid.
