Process of making substrate-limited doughs
Granted: January 31, 1995
Patent Number:
5385742
A dough for the preparation of yeast-leavened flour products whereby the dough comprises a yeast not capable of fermenting malto and an amount of sugar(s) fermentable by the yeast wherein the maximal amount of CO.sub.2 gas produced during the proof is controlled and limited by the amount of fermentable sugar(s) present in the dough.
Glucose isomerases with an altered pH optimum
Granted: January 24, 1995
Patent Number:
5384257
A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The method is based on altering the pK.sub.a of the metal coordinating ligands or altering the K.sub.ass for the metal binding. New glucose isomerases with an altered pH optimum are provided according to this method. These altered…
Glucose isomerase enzymes and their use
Granted: December 27, 1994
Patent Number:
5376536
New mutant glucose isomerases are provided exhibiting improved properties under application conditions. These glucose isomerases are obtained by expression of a gene encoding said enzyme, having an amino acid sequence which differs at least in one amino acid from the wildtype glucose isomerase. Preferred mutant enzymes are those derived from Actinoplanes missouriensis glucose isomerase.
Mutant microbial .alpha.-amylases with increased thermal, acid and/or alkaline stability
Granted: November 15, 1994
Patent Number:
5364782
Thermostable and acid stable .alpha.-amylases are provided as expression products of genetically engineered .alpha.-amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphite method and enzymatic misincorporation on gapped heteroduplex DNA. The mutant .alpha.-amylases have superior properties, e.g. improved thermostability over a broad pH range, for industrial application in starch…
Cloning and expression of xylanase genes from fungal origin
Granted: October 25, 1994
Patent Number:
5358864
Methods and expression constructs are provided for the cloning and overexpression of xylanases of fungai origin in a selected microbial host cell. Xylanases of fungal origin generally have lower pH optima and remain stable over a broader pH range than do xylanases of bacterial origin. The present invention provides for the high level production of fungal xylanases which are may be used in a variety of industrial applications requiring xylanase activity at a low pH.
Cephem derivatives
Granted: October 25, 1994
Patent Number:
5359058
A cephalosporin compound substituted in the 7-position with a (cyclo)alkylideneammonio group of formula (II) ##STR1## wherein X' is an anion from an acid HX, R.sub.1 and R.sub.2 are individually a C.sub.1 -C.sub.16 alkyl group, or R.sub.1 and R.sub.2 together with the carbon atom to which they are attached form a cycloalkylidene ring with up to 8 carbon atoms and a process for the preparation which compounds are useful intermediates.
Astaxanthin-producing yeast cells, methods for their preparation and their use
Granted: October 18, 1994
Patent Number:
5356810
An isolated pure culture of a strain of Phaffia rhodozyma which produces astaxanthin in an amount of at least 600 .mu.g per g Phaffla rhodozyma dry matter, as determined by HPLC analysis.
9-alpha-hydroxy steroids, process for their preparation, process for the preparation of the corresponding 9(11)-dehydro derivatives and pharmaceutical preparations containing such steroids
Granted: October 4, 1994
Patent Number:
5352809
New 9-alphahydroxy steroids are prepared by the introduction of substituents on the D-ring of 9-alpha-hydroxy-androst-4-ene-3,17-dione. The resulting compounds are useful intermediates in the synthesis of corticosteroids.
Modified prokaryotic glucose isomerase enzymes with altered pH activity profiles
Granted: August 23, 1994
Patent Number:
5340738
A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The method is based on altering the pK.sub.a of the metal coordinating ligands or altering the K.sub.ass for the metal binding. New glucose isomerases with an altered pH optimum are provided according to this method. These altered…
PB92 serine protease muteins and their use in detergents
Granted: August 9, 1994
Patent Number:
5336611
New proteolytic enzymes are provided exhibiting improved properties for application in detergents, especially laundry detergents. These enzymes are obtained by expression of a gene encoding a proteolytic enzyme having an amino acid sequence which differs at least in one amino acid from the wild type enzyme. Preferred enzymes are certain mutants derived from the serine protease of Bacillus nov. spec. PB92.
Oxido reductase enzyme system obtained from P. chrysogenum
Granted: July 12, 1994
Patent Number:
5328839
A new oxido reductase enzyme activity obtainable from P. chrysogenum, involved in the production of .beta.-lactams, the set of genes encoding said enzyme activity, and a method to enhance said production by using the above-mentioned oxido reductase system and other oxido reductase systems or genes encoding for the same have been disclosed.
Recombinant genetic means for the production of serine protease muteins
Granted: June 28, 1994
Patent Number:
5324653
New proteolytic enzymes are provided exhibiting improved properties for application in detergents, especially laundry detergents. These enzymes are obtained by expression of a gene encoding a proteolytic enzyme having an amino acid sequence which differs at least in one amino acid from the wild type enzyme. Preferred enzymes are certain mutants derived from the serine protease of Bacillus nov. spec. PB92.
Preparation of novel immobilized biocatalysts
Granted: May 24, 1994
Patent Number:
5314814
Immobilized water-insoluble biocatalysts in particulate form comprise living cells, particularly yeast, dispersed in a cross-linked gelling agent. An enzyme, particularly amyloglucosidase, may be co-immobilized in the particles. These particles are prepared by suspending the living cells in an aqueous solution of a gelling agent, dispersing this suspension in a water immiscible organic liquid to form a suspension in the liquid of aqueous particles comprising the living cells and gelling…
Recombinant DNA encoding neutral trehalase
Granted: May 17, 1994
Patent Number:
5312909
A transformed yeast is disclosed, comprising at least one gene encoding neutral trehalase or trehalose-6-phosphate synthase which gene has been modified such that it differs from a corresponding wild-type gene encoding neutral trehalase or trehalose-6-phosphate synthase, the yeast having as a result a different trehalose content from the untransformed parent yeast. The sugar resistance and drying resistance of the yeast compared to those of the untransformed strain are thereby improved.
Glucose isomerases having altered substrate specificity
Granted: May 10, 1994
Patent Number:
5310665
A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered substrate specificity. New mutant glucose isomerases with an altered substrate specificity are provided according to this method. These altered properties are useful in starch degradation and in other sugar conversion reactions.
Expression and purification of human interleukin-3 and muteins thereof
Granted: April 19, 1994
Patent Number:
5304637
Methods are provided for improved production of hIL-3 either in glycosylated form from mammalian and yeast cells or in unglycosylated form from prokaryotes. Recombinantly produced human IL-3 is purified in a series of steps, initially employing hydrophobic interaction, followed by ion exchange chromatography and gel filtration.
Oxygen removal with immobilized dried Saccharomyces cerevisiae
Granted: March 29, 1994
Patent Number:
5298264
The oxidative deterioration of water-containing products for human consumption, e.g. beverages and oil and/or fat based products is minimized by introduction of immobilized yeast. Yeast is immobilized in and/or on a solid material which allows only very slow penetration by water. Thin layers material of such a yeast-bearing solid, e.g. paraffin, wax, can be applied to the lining of crown corks. The yeast will retain sufficient viability, even after pasteurization of the contents of a…
Preparation of 9-.alpha.-hydroxy-17-keto steroids using Mycobacterium species CBS 482.86
Granted: March 29, 1994
Patent Number:
5298398
9.alpha.-hydroxy-17-keto steroids are prepared by fermenting steroids having a C-17-side chain of 5-10 carbon atoms inclusive, with the novel Mycobacterium species CBS 482.86. Starting from various substrates containing a 5-10 C carbon chain on C-17, 9.alpha.-hydroxyandrost-4-ene-3,17-dione, 7.alpha.,9.alpha.,12.alpha.-trihydroxyandrost-4-ene-3,17-dione or 9.alpha.-hydroxyandrost-4,11-diene-3,17-dione is obtained in high yield. The resulting compounds are useful intermediates in the…
Methods and means for controlling the stability of proteins
Granted: March 1, 1994
Patent Number:
5290690
The invention pertains to a method for the production of a biologically active modified protein derived from a starting protein having essentially the same kind of biological activity with an attendant modulation effect on, particularly increase of, the stability as compared with that of the starting protein. The method comprises substituting an arginine residue for a lysine residue of the starting protein at a site that can sterically accommodate the substitution, without substantially…
Molecular cloning and expression of gene encoding lipolytic enzyme
Granted: January 11, 1994
Patent Number:
5278066
Novel microbial host strains are provided which are transformed by a vector molecule comprising a DNA fragment encoding a lipolytic enzyme and a marker for selection, capable of producing active lipase. Said DNA fragment is preferably derived from a Pseudomonas species.
