Production of enzymes in seeds and their use
Granted: August 6, 1996
Patent Number:
5543576
A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also…
Stabilization of carboxyl esterase
Granted: July 23, 1996
Patent Number:
5538876
Carboxyl esterase is inactivated by several chemical compounds such as naproxen or diclofop. By substituting or modifying certain basic residues of the carboxyl esterase, this enzyme shows improved stability properties during application. In this way it is possible to perform stereospecific hydrolysis reactions on industrial scale even at high substrate concentrations.
Compositions and methods for making lipolytic enzymes
Granted: June 25, 1996
Patent Number:
5529917
Novel microbial host strains are provided which are transformed by a vector molecule comprising a DNA fragment encoding a lipolytic enzyme and a marker for selection, capable of producing active lipase. Said DNA fragment is preferably derived from a Pseudomonas species.
Process for preparation of .beta.-lactams utilizing a combined concentration of acylating agent plus .beta.-lactam derivative of at least 400 mm
Granted: June 11, 1996
Patent Number:
5525483
.beta.-Lactam derivatives are synthesized by an enzymatic reaction of the parent amino .beta.-lactam with the corresponding acylating agent, the concentration of the acylating agent plus the concentration of .beta.-lactam derivative in the reaction mixture being above about 400 mM.
N- and C-terminal truncation and deletion mutants of human interleukin-3
Granted: May 14, 1996
Patent Number:
5516512
Biologically active deletion and substitution mutants of hIL-3 are provided. Preferred mutants are those having one or more deletions at the N-terminus (amino acids 1-14) and/or the C-terminus (amino acids 116-133, 120-130 and/or 130-133). Preferred substitution mutants include Cys.sup.16 .fwdarw.Ala.sup.16 and/or Cys.sup.84 .fwdarw.Ala.sup.84, Glu.sup.50 .fwdarw.Lys.sup.50 and Lys.sup.79 .fwdarw.Glu.sup.79. These mutants can be used to formulate pharmaceutical compositions. Also…
Gram-negative alkaliphilic microorganisms
Granted: May 7, 1996
Patent Number:
5514575
Gram-negative bacteria, which are obligate alkaliphiles, have been isolated from samples of soil, water and sediment and a number of other sources obtained from in and around soda lakes. These bacteria have been analyzed according to the principles of numerical taxonomy with respect to each other, as well as to a variety of known bacteria. In addition, these bacteria are further circumscribed by an analysis of various chemotaxonomic characteristics. The bacteria produce various…
Tortilla
Granted: April 23, 1996
Patent Number:
5510126
The quality of wheat flour tortillas is improved by using a yeast derivative in the tortilla dough.
Vectors for use in filamentous fungi
Granted: April 2, 1996
Patent Number:
5503991
Novel vectors are disclosed for use in filamentous fungi such as Aspergillus sp. in particular, whereby protein coding regions may be inserted therein to achieve expression or expression followed by secretion of the coded protein from the host. Signal peptide sequences and promoter sequences valuable for this purpose are disclosed as are expression vectors containing coding regions native or foreign to the fungal host. In accordance with the invention, a filamentous fungus such as…
Unit for the detection of residues of antibacterial compounds in liquids
Granted: February 27, 1996
Patent Number:
5494805
The detection of residues of antibacterials such as antibiotics and sulpha compounds in liquids such as milk, water, meat juice, serum or urine is disclosed. A test unit comprises an agar medium inoculated with a suitable test organism and two or more redox indicators.
Method for the preparation of certain .beta.-lactam antibiotics
Granted: November 28, 1995
Patent Number:
5470717
The present invention relates to an improved method for the preparation of certain .beta.-lactam antibiotics by enzymatic acylation or by deprotection of a protected intermediate.
Method and compositions for enhancing production of secondary metabolites using clustered biosynthetic genes
Granted: October 31, 1995
Patent Number:
5462862
Clustered antibiotic biosynthetic genes are employed for improvement of production of the antibiotic in microorganisms and for the isolation of other genes involved in the biosynthesis of the antibiotic. The invention is exemplified with improved production of penicillin in Penicillium chrysogenum, with the isolation of another clustered biosynthetic gene(s) and with the expression of penicillin biosynthetic genes in Acremonium chrysogenum.
Gram-negative alkaliphilic microorganisms
Granted: October 17, 1995
Patent Number:
5459062
Gram-negative bacteria, which are obligate alkaliphiles, have been isolated from samples of soil, water and sediment and a number of other sources obtained from in and around soda lakes. These bacteria have been analyzed according to the principles of numerical taxonomy with respect to each other, as well as to a variety of known bacteria. In addition, these bacteria are further circumscribed by an analysis of various chemotaxonomic characteristics. The bacteria produce various…
Mutated .beta.-lactam acylase genes
Granted: October 10, 1995
Patent Number:
5457032
New mutant .beta.-lactam acylases are provided exhibiting altered substrate specificities. These .beta.-lactam acylases are obtained by expression of a gene encoding said .beta.-lactam acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type .beta.-lactam acylase.
Yeast derivative and method to improve bread quality
Granted: September 19, 1995
Patent Number:
5451413
The present invention relates to a method for improving the rheological properties of dough and quality of baked products by addition of a yeast derivative, preferably in combination with a reducing agent and/or enzyme preparations having amylase, hemicellulase, oxido reductase and/or protease activities.
Large scale separation and purification of fermentation product
Granted: August 29, 1995
Patent Number:
5445949
A method of separating a hydrophobic fermentation product selected from the group consisting of lipase, esterase, endoxylanase and an antibiotic from a mixture comprising said product and contaminants which method comprises adding to the mixture sequentially(1) 0.5 to 15% (w/v) of a nonionic surfactant,(2) 0.5 to 60 mg of a flocculating agent per gram of said mixture,(3) 1 to 20% (w/v) of an extra nonionic surfactant,(4) a suitable K, Na, NH.sub.4 or Mg salt selected from the group of…
Cloning and expression of phytase from aspergillus
Granted: July 25, 1995
Patent Number:
5436156
A nucleotide sequence encoding phytase has been isolated and cloned. The coding sequence has been inserted into an expression construct which in turn has been inserted into a vector capable of transforming a microbial expression host. The transformed microbial hosts may be used to economically produce phytase on an industrial scale. The phytase produced via the present invention may be used in a variety of processes requiring the conversion of phytate to inositol and inorganic phosphate.
Detection of antibiotics
Granted: July 18, 1995
Patent Number:
5434053
A process for the detection of antibiotics in a liquid medium such as milk, urine and blood is disclosed which comprisesbringing together a fluid sample of the liquid medium, an labelled antibiotic binding protein, and an immobilized antibiotic,allowing the labelled antibiotic binding protein to bind with the immobilized antibiotic,removing labelled antibiotic binding protein which is not bound to immobilized antibiotic, anddetermining the amount of the labelled antibiotic binding…
Cloning and expression of acetyl xylan esterases from fungal origin
Granted: June 20, 1995
Patent Number:
5426043
Methods and DNA constructs are provided for the expression of a fungal acetyl xylan esterase gene in microbial hosts. A purified fungal acetyl xylan esterase is obtained which is suited for the use as an accessory enzyme in the degradation of acetylated xylans.
Immobilized biocatalysts and their preparation and use
Granted: April 11, 1995
Patent Number:
5405764
Immobilized water-insoluble biocatalysts in particulate form comprise living cells, particularly yeast, dispersed in a cross-linked gelling agent. An enzyme, particularly amyloglucosidase, may be co-immobilized in the particles. These particles are prepared by suspending the living cells in an aqueous solution of a gelling agent, dispersing this suspension in a water immiscible organic liquid to form a suspension in the liquid of aqueous particles comprising the living cells and gelling…
Gram-positive alkaliphilic microorganisms
Granted: March 28, 1995
Patent Number:
5401657
The present invention provides novel aerobic, Gram-positive alkaliphilic bacteria which have been isolated from in and around alkaline soda lakes. These alkaliphiles have been analyzed according to the principles of numerical taxonomy with respect to each other and also to a collection of known bacteria. In addition, these bacterial taxa are further circumscribed by an analysis of the lipid components which serve as chemotaxonomic markers. The alkaliphiles of the present invention…
