Immobilized biocatalysts
Granted: August 11, 1992
Patent Number:
5137818
Immobilized water-insoluble biocatalysts in particulate form comprise living cells, particularly yeast, dispersed in a cross-linked gelling agent. An enzyme, particularly amyloglucosidase, may be co-immobilized in the particles. These particles are prepared by suspending the living cells in an aqueous solution of a gelling agent, dispersing this suspension in a water immiscible organic liquid to form a suspension in the liquid of aqueous particles comprising the living cells and gelling…
Infectious bronchitis vaccine for poultry
Granted: July 28, 1992
Patent Number:
RE34013
Infectious bronchitis (IB) vaccines for poultry derived from at least one novel virus strain of novel infectious bronchitis serotypes, selected from the group consisting of culture Nos. CNCTC A 07/80, CNCTC A 08/80, CNCTC A 09/80, CNCTC A 010/80, CNCTC A 011/80, CNCTC A 013/80, CNCTC A 014/80, CNCTC A 015/80 and CNCTC A 016/80 deposited at the Czechoslovak National Collection of Type Cultures of the Institute of Hygiene and Epidemiology in Prague, Czechoslovakia and the novel viruses per…
Fluidized bed process
Granted: May 26, 1992
Patent Number:
5116505
The present invention relates to a process for the anaerobic purification of waste water which comprises a fluidized-bed process in which granular sludge is applied.Moreover apparatus is disclosed for the purification of waste water using a fluidized-bed reactor wherein a liquid introduction device is present which is formed by one or more separate inlet pipes, the pipes delivering the liquid near the bottom of the reactor with an outlet velocity of 0.5-4 m/s, preferably 1-2 m/s.
Process for the preparation of ibuprofen
Granted: April 28, 1992
Patent Number:
5108917
A process is provided for the preparation of ibuprofen enriched in R isomer, which comprises subjecting an ester of ibuprofen, to the action of a micro-organism or substances derived thereof, that will stereoselectively hydrolyse the ester to form ibuprofen predominantly having R configuration.
Method for identifying and using biosynthetic genes for enhanced production of secondary metabolites
Granted: April 28, 1992
Patent Number:
5108918
Novel methods and compositions are provided for the enhanced production of secondary metabolites. Methods are provided for identifying sequences which when transformed into secondary metabolite producing hosts, enhance the production of secondary metabolite. The process is exemplified for penicillin. In addition, the P. chrysogenum acyltransferase gene is isolated and sequenced.
Process for the preparation of 3-exomethylene cepham derivatives
Granted: April 28, 1992
Patent Number:
5109132
A process is provided for the preparation of 3-methylene and 3-halomethylene cepham derivatives via 3-phosphoniomethyl-3-cephem derivatives, which in turn can be prepared from 3-halomethyl-3-cephem derivatives. The preparation can be carried out with or without isolating the intermediate phosphonium cephem compounds. The 3-methylene and 3-halomethylene cepham derivatives and in particular the 1-oxo and 1,1-dioxo cepham compounds are also prepared by reducing a 3-halomethyl-3-cepham…
Dry yeast immobilized in wax or paraffin for scavenging oxygen
Granted: April 21, 1992
Patent Number:
5106633
A food-grade oxygen scavenger for removing oxygen from foods and beverages in containers is prepared by immobilizing dried yeast containing at least 92% dry matter in a solid material such as wax or paraffin. The solid material allows for very slow penetration of water and permits the yeast to contact only water that penetrates therethrough. The immobilized yeast is coated on an inside surface of a container or on a surface of a closure such as a stopper that is on the inside of the…
Pharmaceutical granulate
Granted: February 4, 1992
Patent Number:
5085869
A wet granulate is provided for therapeutically useful substances having a water solubility 10 wt % or less and microcrystalline or microfine cellulose or a mixture of both, where substantial amounts of wet binders are avoided.Tableting mixtures with the new granulate possess a good flow pattern and may be compressed to tablets which show an excellent disintegration behavior.
Anoxic ammonia oxidation
Granted: January 7, 1992
Patent Number:
5078884
A biological denitrification is provided in which ammonium ion is used as an electron donor in the denitrification. The process may advantageously be used under conditions of high ammonium ion concentrations in waste water or to remove ammonium ion from manure, soil or surface water. Microorganisms are described which may be used in these processes.
Process for the preparation of 2-aryloxypropionic acids
Granted: December 24, 1991
Patent Number:
5075233
A process is provided for the enrichment in R isomer of an aryloxypropioic acid ester and for the stereoselective hydrolysis of an aryloxypropionic acid ester to form the corresponding aryloxypropionic acid in predominantly S configuration which comprises subjecting an aryloxypropionic acid ester of formula I ##STR1## wherein A is an ester residue and preferably an alkyl group, optionally substituted or branched, wherein R' is an optionally substituted aryl or heterocyclic ring system,…
Process for microencapsulation
Granted: November 19, 1991
Patent Number:
5066436
A process for microencapsulating bioactive substances in biocompatible polymers according to the phase-separation principle, wherein an excess of an ethyl or isopropyl ester of a straight-chain fatty acid having 12-18 carbon atoms is used as the hardening liquid, and the pharmaceutical compositions containing the microcapsules prepared by this process. Isopropyl myristate and isopropyl palmitate are preferred hardening liquids.
Preparation of 3-exomethylenecepham sulfoxides and 2-chlorosulfinylazetidinones using molecular sieves
Granted: October 1, 1991
Patent Number:
5053501
3-Exomethylenecepham sulfoxide derivatives and intermediates therefor, viz. 2-chlorosulfinylazetidin-4-ones are prepared in high yield and purity by reaction of a penam sulfoxide ester with a N-chloro halogenating agent in the presence of molecular sieves.
Microbial purified esterases
Granted: August 6, 1991
Patent Number:
5037751
A process for the preparation of a pharmaceutically active compound in a stereospecific form of the formula ##STR1## or a pharmaceutically acceptable salt or ester thereof, like an alkali metal salt or an alkaline earth metal salt or a pivaloyl ester, wherein R.sub.1 represents an optionally substituted aryl group such as a phenyl or naphthyl group optionally included in a heterocyclic ring system, which is optionally substituted, or represents a heteroaromatic ring system containing in…
Process for the preparation of substituted phenoxy propanoic acids
Granted: August 6, 1991
Patent Number:
5037759
The R-enantiomer of a compound of formula I: ##STR1## where Z is an optionally substituted cyclic organic moiety and C* represents an optically active carbon atom, may be prepared by supplying a substrate of formula II ##STR2## to a culture of a microorganism capable of oxidation of compound II into the R-enantiomer of I.
Secretory signal selection vectors for extracellular protein synthesis bacilli
Granted: August 6, 1991
Patent Number:
5037760
Novel vectors are provided for identifying secretory signal sequences from DNA fragments of unicellular microorganisms. The plasmids comprise a multiple cloning site with restriction sites in reading frame with a structural gene which permits rapid screening of the screted expression product. Optionally, the vectors may include a promoter region upstream from the multiple cloning site. The invention is exemplified with Bacillus. Specific secretory signal sequence have been isolated with…
Regulatory region cloning and analysis plasmid for bacillus
Granted: June 18, 1991
Patent Number:
5024943
Novel constructs in plasmids are provided for evaluating the efficiency of expression and secretion of structural genes. The constructs provide for transcriptional and translational regulatory regions, a signal sequence and structural gene, which also may be readily excised and substituted, so as to allow for mixing and matching of regulatory regions, signal sequences and genes to evaluate regions for use in the expression of a desired peptide. Particularly, synthetic regions are…
Retarding the firming of bread crumb during storage
Granted: June 11, 1991
Patent Number:
5023094
Retardation of crumb firming during storage of a bread product by the incorporation of an effective amount of a thermostable glucanase into the dough before baking which thermostable glucanase restrains the retrogradation of the amylopectin without substantially affecting the amylose component of the starch.
Production of glucose syrups and purified starches from wheat and other cereal starches containing pentosans
Granted: June 11, 1991
Patent Number:
5023176
The filterability of glucose syrups obtained from impure wheat or other cereal starch is improved by treatment with Disporotrichum. Also the separation of starch from other constituents of impure cereal starch is improved by addition of xylanase before the starch is separated.
Yeast package
Granted: May 28, 1991
Patent Number:
5019410
A yeast package is provided which can be used during the second fermentation in a bottle for the production of sparkling wines starting from basic wine containing sugar. A drastic reduction in time and in labor is possible due to easy separation of yeast and beverage.
DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
Granted: April 23, 1991
Patent Number:
5010182
DNA constructs that are useful for providing secretory expression of heterologous polypeptides in yeast comprising a DNA sequence that includes a Kluyveromyces .alpha.-factor leader sequence linked to the heterologous polypeptide by a yeast processing signal. Constructs employing the K. lactis .alpha.-factor leader and processing signal sequences, with and without spacer, linked to prochymosin are exemplified.
