NONIONIC POLYETHER SURFACTANTS
Granted: October 17, 2024
Application Number:
20240343700
Non-ionic polyether surfactants with improved resistance to hydrolysis are provided.
USE OF DESTABILIZING NUCLEOTIDE TRIPHOSPHATES TO REDUCE AMPLICON FOLDING AND Tm
Granted: October 10, 2024
Application Number:
20240336961
Described herein are methods and compositions that provide highly efficient primer annealing and denaturation during nucleic acid amplification. The basic method employs destabilizing nucleotide triphosphates (dNTPs) in amplification reaction mixtures. The incorporation of such dNTPs into the amplicon produced upon amplification lowers the Tm of the amplicon, promoting denaturation/strand separation for the next round of primer annealing.
Methods of Detecting Bladder Cancer
Granted: August 29, 2024
Application Number:
20240287618
Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of detecting low grade bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting androgen receptor (AR) and/or uroplakin 1B (UPK1B).
MPOX CLADE II BIOMARKER PANEL
Granted: July 4, 2024
Application Number:
20240218467
The present disclosure provides a set of primers and optional probes for identifying the presence of MPOX virus, Clade II, which can be included with (e.g., in at kit) or in a cartridge for automated detection of these pathogens by nucleic acid amplification. This disclosure also provides related detection methods, as well as cartridges, systems, and kits useful in such methods.
Methods of Detecting Bladder Cancer
Granted: May 30, 2024
Application Number:
20240175089
Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting a set of markers consisting of CRH, IGF2, KRT20, and ANXA10.
INTEGRATED PURIFICATION AND MEASUREMENT OF DNA METHYLATION AND CO-MEASUREMENT OF MUTATIONS AND/OR MRNA EXPRESSION LEVELS IN AN AUTOMATED REACTION CARTRIDGE
Granted: May 2, 2024
Application Number:
20240141415
In various embodiments methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA;…
EXPONENTIAL BASE-GREATER-THAN-BASE 2 NUCLEIC ACID AMPLIFICATION USING HIGH- AND LOW-Tm PRIMERS
Granted: March 28, 2024
Application Number:
20240102085
Described herein are methods and compositions that provide highly efficient nucleic acid amplification. The method employs pairs of primers that differ significantly in Tm and a novel temperature/time course characterized by a temperature pulse during denaturation that enables a high-Tm primer (but not a low-Tm primer) to anneal and prime the synthesis of an additional nucleic acid strand beyond the two strands synthesized in a cycle of classical PCR. In some embodiments, this allows a…
PSEUDO-COMPLEMENTARY BASES IN GENOTYPING AND NUCLEIC ACID SEQUENCING
Granted: June 1, 2023
Application Number:
20230167490
Described herein are methods and compositions that make use of pseudo-complementary bases to reduce unwanted hybridization in assays to detect and/or quantify particular nucleotide sequences, as well as in nucleic acid sequencing protocols.
EXPONENTIAL BASE-3 AND GREATER NUCLEIC ACID AMPLIFICATION WITH REDUCED AMPLIFICATION TIME
Granted: March 30, 2023
Application Number:
20230096557
Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Modified bases can be employed in primers to provide this base-3 or greater amplification with satisfactory PCR cycle times, which are improved, as compared to those observed in the absence of modified…
THERMOSTABLE POLYMERASE INHIBITOR COMPOSITIONS AND METHODS
Granted: December 8, 2022
Application Number:
20220389427
The present disclosure relates to aptamers for temperature-dependent reversible inhibition of thermostable polymerase activity in order to improve sensitivity and specificity of various reactions and assays involving hot start polynucleotide synthesis. Methods for use of the aptamers and related compositions and kits are also provided.
Methods of Detecting Trichomonas Vaginalis
Granted: September 15, 2022
Application Number:
20220290260
Compositions and methods for detecting Trichomonas vaginalis are provided.
METHODS OF DIAGNOSING TUBERCULOSIS AND DIFFERENTIATING BETWEEN ACTIVE AND LATENT TUBERCULOSIS
Granted: April 7, 2022
Application Number:
20220106627
Compositions and methods for detecting Mycobacterium tuberculosis (MTB) infection in a patient suspected of being infected with Mycobacterium tuberculosis and for distinguishing between active and latent tuberculosis infection are provided. The methods may also be used to monitor progression of MTB infection or to monitor treatment of MTB infected patients. Changes in the expression level of genes are used to aid in the diagnosis, prognosis and treatment of tuberculosis.
METHODS OF DETECTING SARS-COV-2, INFLUENZA, AND RSV
Granted: January 20, 2022
Application Number:
20220017979
Compositions and methods for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), influenza A, influenza B, and respiratory syncytial virus (RSV) are provided.
FLUORESCENT DYES
Granted: January 20, 2022
Application Number:
20220017531
Automated oligonucleotide synthesis-compatible fluorescent dye phosphoramidite compounds, solid supports, and labeled polynucleotides incorporating the compounds are provided. The compounds allow universal incorporation of the fluorescent label into any position of the polynucleotide.
NONIONIC POLYETHER SURFACTANTS
Granted: January 20, 2022
Application Number:
20220017480
Non-ionic polyether surfactants with improved resistance to hydrolysis are provided.
METHODS AND COMPOSITIONS FOR NUCLEIC ACID ISOLATION
Granted: October 21, 2021
Application Number:
20210324372
Methods and compositions for isolation of nucleic acids from biological samples are provided.
NUCLEIC ACID ISOLATION AND RELATED METHODS
Granted: August 5, 2021
Application Number:
20210238583
Solid supports modified with pectins derivatives are provided. The solid supports are useful in nucleic acid isolation, separation, and detection methods.
NUCLEIC ACID DECONTAMINATION METHODS
Granted: June 3, 2021
Application Number:
20210162085
Methods and cleaning compositions for reduction of nucleic acid contamination on surfaces, in air, and in solutions using modified pectin are provided.
EXPONENTIAL BASE-3 AND GREATER NUCLEIC ACID AMPLIFICATION WITH REDUCED AMPLIFICATION TIME
Granted: July 30, 2020
Application Number:
20200239878
Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Modified bases can be employed in primers to provide this base-3 or greater amplification with satisfactory PCR cycle times, which are improved, as compared to those observed in the absence of modified…
Methods of Detecting Influenza
Granted: April 2, 2020
Application Number:
20200102620
Compositions and methods for detecting influenza are provided.
