REAGENT RESERVOIR SYSTEM FOR ANALYTICAL INSTRUMENTS
Granted: April 4, 2013
Application Number:
20130084631
The invention provides a reagent reservoir system and disposable reaction cassettes using the same. In one aspect, such system comprises a chamber in which dried reagent, particularly lyophilized reagent, is constrained to remain in a defined region of the chamber by a retaining member that obstructs passage of such reagents to other regions of the chamber where they may escape hydration or activation.
METHODS OF DETECTING SEPSIS
Granted: April 4, 2013
Application Number:
20130084343
Methods of sepsis in a sample from a patient are provided. Methods of detecting changes in expression of one or more RNAs associated with sepsis are also provided. Compositions and kits are also provided.
DNA RECOMBINATION JUNCTION DETECTION
Granted: March 14, 2013
Application Number:
20130065774
The present invention provides methods, compositions and kits for detecting the presence or absence of an integrated insertion polynucleotide.
METHOD FOR SEPARATING AN ANALYTE FROM A SAMPLE
Granted: November 22, 2012
Application Number:
20120295269
An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is…
CARTRIDGE FOR CONDUCTING A CHEMICAL REACTION
Granted: November 15, 2012
Application Number:
20120288924
A cartridge for conducting a chemical reaction includes a body having at least one flow path formed therein. The cartridge also includes a reaction vessel extending from the body for holding a reaction mixture for chemical reaction and optical detection. The vessel comprises a rigid frame defining the side walls of a reaction chamber. The frame includes at least one channel connecting the flow path to the chamber. The vessel also includes flexible films or sheets attached to opposite…
METHODS OF DETECTING LUNG CANCER
Granted: September 27, 2012
Application Number:
20120244530
Methods of detecting lung cancer, such as non-small cell lung cancer, including squamous cell carcinoma and adenocarcinoma, are provided. Methods of detecting changes in the levels of one or more small RNAs associated with lung cancer are also provided. Compositions and kits are also provided.
METHODS FOR THE IDENTIFICATION OF MICRORNA AND THEIR APPLICATIONS IN RESEARCH AND HUMAN HEALTH
Granted: July 5, 2012
Application Number:
20120171686
The press invention concerns a method for prediction and identification of microRNA precursors (pre-microRNA) and microRNA molecules using data processing programs and databases. The invention also pertains to the isolated form of these pre-microRNAs, microRNA molecules and derived nucleic acids there of. The invention also relates to recombinant vector, host cell, support, pharmaceutical composition or kit comprising such microRNA molecules or there of derivated molecules. The invention…
Device for extracting nucleic acid from a sample
Granted: July 5, 2012
Application Number:
20120171758
The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or…
FLUID PROCESSING AND CONTROL
Granted: February 9, 2012
Application Number:
20120034705
A fluid control and processing system for controlling fluid flow among a plurality of chambers comprises a body including a fluid processing region continuously coupled fluidicly with a fluid displacement region. The fluid displacement region is depressurizable to draw fluid into the fluid displacement region and pressurizable to expel fluid from the fluid displacement region. The body includes at least one external port. The fluid processing region is fluidicly coupled with the at least…
APPARATUS FOR PERFORMING HEAT-EXCHANGING CHEMICAL REACTIONS
Granted: July 21, 2011
Application Number:
20110176971
An apparatus for controlling the temperature of a reaction mixture contained in a chamber of a reaction vessel comprises a thermal surface for contacting a flexible wall of the chamber and an automated machine for increasing the pressure in the chamber. The pressure increase in the chamber is sufficient to force the flexible wall to conform to the thermal surface for good thermal conductance. The apparatus also includes at least one thermal element for heating or cooling the surface to…
POLAR DYES
Granted: March 3, 2011
Application Number:
20110053155
The present invention relates to novel polar fluorescent and quenchers dyes, and minor groove binder with enhanced polarity. The present invention further relates to methods of preparing oligonucleotide probes labeled with polar arsonate dyes under the condition of automated synthesis and method of using such probes.
Methods of Detecting Cervical Cancer
Granted: September 23, 2010
Application Number:
20100240049
Methods of detecting cervical dysplasia, such as cervical dysplasia likely to progress to carcinoma in a sample of human cervical cells, are provided. Methods of detecting changes in expression of one or more microRNAs or mRNAs associated with cervical dysplasia or cervical cancer are also provided. Compositions and kits are also provided.
METHODS OF DETECTING LUNG CANCER
Granted: September 16, 2010
Application Number:
20100233704
Methods of lung cancer in a sample from a patient are provided. Methods of detecting changes in expression of one or more target RNAs associated with lung cancer are also provided. Compositions and kits are also provided.
COMPOSITIONS, METHODS AND KITS FOR POLYNUCLEOTIDE AMPLIFICATION REACTIONS AND MICROFLUIDIC DEVICES
Granted: June 3, 2010
Application Number:
20100136569
Antifoam agents improve detection of polynucleotide amplification reactions and improve manipulation of fluids in microfluidic devices.
METHOD AND DEVICE FOR SAMPLE PREPARATION CONTROL
Granted: May 27, 2010
Application Number:
20100129827
A method for preparing a sample suspected to contain a target nucleic acid sequence for a nucleic acid amplification reaction and for verifying the effectiveness of the sample preparation comprises the step of mixing the sample with sample preparation controls. The sample preparation controls are cells, spores, microorganisms, or viruses that contain a marker nucleic acid sequence. The sample mixed with the sample preparation controls is subjected to a lysis treatment, and nucleic acid…
METHOD FOR SEPARATING AN ANALYTE FROM A SAMPLE
Granted: March 18, 2010
Application Number:
20100068706
An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is…
METHOD AND APPARATUS FOR STORING AND DISPENSING REAGENT BEADS
Granted: December 17, 2009
Application Number:
20090308886
Embodiments of the invention provide an efficient and effective technique for storing and dispensing reagent beads. In some embodiments, an apparatus is provided for dispensing reagent beads contained in a bead storage device which includes a bead carrier having a plurality of wells; a plurality of reagent beads disposed in the wells; and a cover tape releasably attached to the bead carrier to cover the wells and retain the reagent beads in the wells. The apparatus comprises a channel in…
METHODS FOR THE IDENTIFICATION OF MICRORNA AND THEIR APPLICATIONS IN RESEARCH AND HUMAN HEALTH
Granted: March 5, 2009
Application Number:
20090062135
The present invention concerns a method for prediction and identification of microRNA precursors (pre-microRNA) and microRNA molecules using data processing programs and databases. The invention also pertains to the isolated form of these pre-microRNAs, microRNA molecules and derived nucleic acids there of. The invention also relates to recombinant vector, host cell, support, pharmaceutical composition or kit comprising such microRNA molecules or there of derivated molecules. The…
DNA recombination junction detection
Granted: February 19, 2009
Application Number:
20090047669
The present invention provides methods, compositions and kits for detecting the presence or absence of an integrated insertion polynucleotide.
APPARATUS FOR PERFORMING HEAT-EXCHANGING CHEMICAL REACTIONS
Granted: November 20, 2008
Application Number:
20080286151
An apparatus for controlling the temperature of a reaction mixture contained in a chamber of a reaction vessel comprises a thermal surface for contacting a flexible wall of the chamber and an automated machine for increasing the pressure in the chamber. The pressure increase in the chamber is sufficient to force the flexible wall to conform to the thermal surface for good thermal conductance. The apparatus also includes at least one thermal element for heating or cooling the surface to…
