PEROXIDASE ACTIVITY TOWARDS 10-ACETYL-3,7-DIHYDROXYPHENOXAZINE
Granted: September 12, 2024
Application Number:
20240301367
The present invention provides engineered peroxidase enzymes, polypeptides having peroxidase activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing peroxidase enzymes are also provided. The present invention further provides compositions comprising the peroxidase enzymes and methods of using the engineered peroxidase enzymes. The present invention finds particular use in the…
ENGINEERED GALACTOSE OXIDASE VARIANT ENZYMES
Granted: August 29, 2024
Application Number:
20240287474
The present invention provides engineered galactose oxidase (GOase) enzymes, polypeptides having GOase activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing GOase enzymes are also provided. The present invention further provides compositions comprising the GOase enzymes and methods of using the engineered GOase enzymes. The present invention finds particular use in the production of…
ENGINEERED IMINE REDUCTASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS
Granted: August 1, 2024
Application Number:
20240254457
The present application provides engineered polypeptides having imine or oxime reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
ENGINEERED 3-O-KINASE VARIANTS AND METHODS OF USE
Granted: June 27, 2024
Application Number:
20240209408
The present invention provides engineered 3?O-kinase polypeptides useful for construction of materials used in template-independent polynucleotide synthesis, as well as compositions and methods of utilizing these engineered polypeptides. The present disclosure also describes one-pot methods for conversion of a natural or modified nucleoside to a nucleoside tetraphosphate or NQP.
Engineered Terminal Deoxynucleotidyl Transferase Variants
Granted: June 6, 2024
Application Number:
20240182876
The present invention provides engineered terminal deoxynucleotidyl transferase (TdT) polypeptides useful in template-independent polynucleotide synthesis, as well as compositions, methods of utilizing these engineered polypeptides, and polynucleotides encoding the engineered terminal deoxynucleotidyl transferases.
AUTOMATED SCREENING OF ENZYME VARIANTS
Granted: February 16, 2023
Application Number:
20230048421
Disclosed are methods for identifying bio-molecules with desired properties (or which are most suitable for a round of directed evolution) from complex bio-molecule libraries or sets of such libraries. Some embodiments of the present disclosure provide methods for virtually screening proteins for beneficial properties. Some embodiments of the present disclosure provide methods for virtually screening enzymes for desired activity and/or selectivity for catalytic reactions involving…
AUTOMATED SCREENING OF ENZYME VARIANTS
Granted: February 16, 2023
Application Number:
20230048421
Disclosed are methods for identifying bio-molecules with desired properties (or which are most suitable for a round of directed evolution) from complex bio-molecule libraries or sets of such libraries. Some embodiments of the present disclosure provide methods for virtually screening proteins for beneficial properties. Some embodiments of the present disclosure provide methods for virtually screening enzymes for desired activity and/or selectivity for catalytic reactions involving…
AUTOMATED SCREENING OF ENZYME VARIANTS
Granted: February 16, 2023
Application Number:
20230048421
Disclosed are methods for identifying bio-molecules with desired properties (or which are most suitable for a round of directed evolution) from complex bio-molecule libraries or sets of such libraries. Some embodiments of the present disclosure provide methods for virtually screening proteins for beneficial properties. Some embodiments of the present disclosure provide methods for virtually screening enzymes for desired activity and/or selectivity for catalytic reactions involving…
ENGINEERED IMINE REDUCTASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS
Granted: December 14, 2017
Application Number:
20170355966
The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
N-SUBSTITUTED 4-AMINOQUINAZOLINE DERIVATIVES AND METHODS OF USE
Granted: November 23, 2017
Application Number:
20170334886
The present invention provides kinase inhibitor analogs with improved properties, such as improved efficacy, pharmacokinetics, safety, and specificity. In some embodiments, the present invention provides lapatinib analogs that provide therapeutic benefits.
PROTEIN VARIANT GENERATION BY REGION SHUFFLING
Granted: November 3, 2016
Application Number:
20160319273
Region shuffling methods for efficiently introducing diversity and exploring sequence space are described. Libraries produced directly from these methods contain high fractions of protein variants harboring multiple beneficial mutations. Typically, the methods produce these variants efficiently without the need for sequencing beneficial mutants identified at intermediate stages of the process.
METHOD OF DEMULSIFYING AND PURIFYING ORGANIC PRODUCTS FROM AN EMULSION
Granted: February 25, 2016
Application Number:
20160052846
This invention provides methods to demulsify organic products from emulsions, using demulsifying solvents which act as a combination of demulsifier and low volume extraction solvent. The methods can be applied to purify organic products such as fatty alcohols from emulsions including those generated from fermentation broths.
MODIFIED NATIVE BETA-KETOACYL-ACP SYNTHASES AND ENGINEERED MICROORGANISMS
Granted: January 14, 2016
Application Number:
20160010115
Genetically engineered cells and microorganisms are provided that produce fatty alcohols and fatty acids. In particular, engineered microbial cells comprise a modified native gene having ?-ketoacyl-acp synthase activity.
PRODUCTION OF FATTY ALCOHOLS FROM ENGINEERED MICROORGANISMS
Granted: June 25, 2015
Application Number:
20150176040
Recombinant microorganisms are provided which have been engineered to produce fatty alcohols. Also provided are recombinant microorganisms which comprise a heterologous polynucleotide encoding a fatty alcohol reductase enzyme and an introduced polynucleotide encoding a ?-ketoacyl acyl carrier protein synthase.
ENDOGLUCANASE 1B
Granted: December 4, 2014
Application Number:
20140356914
The present invention provides endoglucanase 1b (EG1b) suitable for use in saccharification reactions.
DIRECT BIOCATALYTIC PRODUCTION OF ACRYLIC ACID AND OTHER CARBOXYLIC ACID COMPOUNDS
Granted: November 20, 2014
Application Number:
20140342414
The present disclosure relates to biocatalytic methods or processes for the synthesis of acrylic acid and its derivatives, or other carboxylic acid compounds of the formula R—CO2H, wherein R is a carbon chain of 5 carbons or fewer, such as methacrylic acid or 3-hydroxypropionic acid. More specifically, the disclosure relates to methods of using an acyl-CoA hydrolase (such as a thioesterase) as a biocatalyst for the hydrolysis (and removal of the CoA moiety) of a substrate acyl-CoA…
BIOCATALYSTS AND METHODS FOR THE SYNTHESIS OF SUBSTITUTED LACTAMS
Granted: November 20, 2014
Application Number:
20140342412
The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.
METHOD OF GENERATING AN OPTIMIZED, DIVERSE POPULATION OF VARIANTS
Granted: September 11, 2014
Application Number:
20140256557
The disclosure relates to a method of generating a diverse set of variants to screen improved and novel properties within the variant population, a system for creating the diverse set of variants, and the variant peptides.
BETA-GLUCOSIDASE VARIANTS
Granted: September 11, 2014
Application Number:
20140256018
The invention relates to recombinantly produced ?-glucosidase variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant ?-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target ?-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.
METHODS, SYSTEMS, AND SOFTWARE FOR IDENTIFYING FUNCTIONAL BIO-MOLECULES
Granted: September 4, 2014
Application Number:
20140249035
The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention…
