Production of Fatty Alcohols with Fatty Alcohol Forming Acyl-CoA Reductases (FAR)
Granted: April 10, 2014
Application Number:
20140099686
The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
Recombinant Beta-Glucosidase Variants for Production of Soluble Sugars from Cellulosic Biomass
Granted: March 6, 2014
Application Number:
20140065678
The invention relates to recombinant expression of a variant form of a fungal C1 strain ?-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the ?-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant ?-glucosidase variant protein,…
FUNGAL BETA-XYLOSIDASE VARIANTS
Granted: February 20, 2014
Application Number:
20140051119
The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using…
PENTOSE FERMENTATION BY A RECOMBINANT MICROORGANISM
Granted: January 16, 2014
Application Number:
20140017765
The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.
PROTEIN VARIANT GENERATION BY REGION SHUFFLING
Granted: January 2, 2014
Application Number:
20140005057
Region shuffling methods for efficiently introducing diversity and exploring sequence space are described. Libraries produced directly from these methods contain high fractions of protein variants harboring multiple beneficial mutations. Typically, the methods produce these variants efficiently without the need for sequencing beneficial mutants identified at intermediate stages of the process.
BETA-GLUCOSIDASE VARIANT ENZYMES AND RELATED POLYNUCLEOTIDES
Granted: January 2, 2014
Application Number:
20140004586
The invention provides variants of the Azospirillum irakense CelA ?-glucosidase that have improve ?-glucosidase activity, particularly improved thermoactivity, compared to the wild type enzyme. The invention further provides related polynucleotides, vectors, host cell, and methods for making and using the variants.
KETOREDUCTASE POLYPEPTIDES FOR THE REDUCTION OF ACETOPHENONES
Granted: December 26, 2013
Application Number:
20130344552
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
COMPOSITIONS AND METHODS FOR PRODUCTION OF FERMENTABLE SUGARS
Granted: December 26, 2013
Application Number:
20130344541
The present application provides genetically modified fungal organisms that produce enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to glucose, enzyme mixtures produced by the genetically modified fungal organisms, and processes for producing glucose from cellulose using such enzyme mixtures.
Engineered imine reductases and methods for the reductive animation of ketone and amine compounds.
Granted: November 14, 2013
Application Number:
20130302859
The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
RECOMBINANT LIGNOCELLULOSE DEGRADATION ENZYMES FOR THE PRODUCTION OF SOLUBLE SUGARS FROM CELLULOSIC BIOMASS
Granted: October 31, 2013
Application Number:
20130288310
The invention relates to C1 lignocellulose degradation enzyme nucleic acid and protein sequences and expression of recombinant C1 lignocellulose degradation enzymes. The invention provides methods for degrading a cellulosic biomass by contacting the biomass with a recombinant C1 lignocellulose degradation enzyme of the invention.
ENGINEERED KETOREDUCTASE POLYPEPTIDES
Granted: August 15, 2013
Application Number:
20130210098
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered…
KETOREDUCTASES AND USES THEREOF
Granted: August 1, 2013
Application Number:
20130196408
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
CHEMICALLY MODIFIED CARBONIC ANHYDRASES USEFUL IN CARBON CAPTURE SYSTEMS
Granted: July 25, 2013
Application Number:
20130189756
The present disclosure relates to chemically modified carbonic anhydrase polypeptides and soluble compositions, homogenous liquid formulations comprising them. The chemically modified carbonic anhydrase polypeptides have improved properties relative to the same carbonic anhydrase polypeptide that is not chemically modified including the improved properties of increased activity and/or stability in the presence of amine compounds, ammonia, or carbonate ion. The present disclosure also…
KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF A 3-ARYL-3 HYDROXYPROPANAMINE FROM A 3-ARYL-3-KETOPROPANAMINE
Granted: July 11, 2013
Application Number:
20130177962
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the…
HIGHLY STABLE BETA-CLASS CARBONIC ANHYDRASES USEFUL IN CARBON CAPTURE SYSTEMS
Granted: July 4, 2013
Application Number:
20130171718
The present disclosure relates to ?-class carbonic anhydrase polypeptides having improved properties including increased thermostability and/or stability in the presence of amine compounds, ammonia, or carbonate ion. The present disclosure also provides formulations and uses of the polypeptides for accelerating the absorption of carbon dioxide from a gas stream into a solution as well as for the release of the absorbed carbon dioxide for further treatment and/or sequestering. Also…
STRUCTURE-ACTIVITY RELATIONSHIPS
Granted: June 27, 2013
Application Number:
20130165341
The present disclosure relates to compositions and methods for screening a plurality of polypeptide variants.
BIOCATALYSTS AND METHODS FOR THE SYNTHESIS OF (1R,2R)-2-(3,4-DIMETHOXYPHENETHOXY)CYCLOHEXAMINE
Granted: June 27, 2013
Application Number:
20130164794
The disclosure provides transaminase polypeptides capable of converting the substrate, 2-(3,4-dimethoxyphenethoxy)cyclohexanone to the trans diastereomer product (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine in at least a 2:1 diastereomeric ratio relative to the cis diastereomer (1R,2S)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine. The disclosure also provides polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides in processes for preparing…
STRUCTURE-ACTIVITY RELATIONSHIPS
Granted: June 20, 2013
Application Number:
20130157900
The present disclosure relates to compositions and methods for screening a plurality of polypeptide variants.
PRODUCTION OF MONOTERPENES
Granted: June 6, 2013
Application Number:
20130143291
The present invention relates to methods for producing monoterpenes, particularly tricyclene, by culturing microbial organisms that express a terpene synthase and optionally a prenyl transferase.
COMBINED AUTOMATED PARALLEL SYNTHESIS OF POLYNUCLEOTIDE VARIANTS
Granted: June 6, 2013
Application Number:
20130143767
The present disclosure relates to methods for efficient synthesis, cloning, transformation and screening of large diverse libraries of polynucleotide variants comprising well-defined nucleotide differences relative to a reference polynucleotide.
