METHODS, SYSTEMS, AND SOFTWARE FOR IDENTIFYING FUNCTIONAL BIO-MOLECULES
Granted: June 30, 2011
Application Number:
20110161265
The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention…
RECOMBINANT THERMOASCUS AURANTIACUS BETA-GLUCOSIDASE VARIANTS FOR PRODUCTION OF FERMENTABLE SUGARS FROM CELLULOSIC BIOMASS
Granted: May 26, 2011
Application Number:
20110124058
The present invention provides compositions and methods for the expression of recombinant ?-glucosidase variants, as well as their use in the production of fermentable sugars from cellulosic biomass.
VARIANT LOVD POLYPEPTIDES AND THEIR USES
Granted: May 12, 2011
Application Number:
20110111468
The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound
REDUCED CODON MUTAGENESIS
Granted: April 7, 2011
Application Number:
20110082055
Methods and compositions that reduce complexity of libraries of variant biological molecules, that reduce oversampling of these libraries during screening and that improve screening efficiency are provided. Sets of efficient degenerate codon sets are provided that efficiently encode all, or nearly all canonical amino acids. Degenerate oligonucleotides comprising these codons are provided, as are polynucleotide variants. Variant pooling strategies are used during library construction.…
METHOD OF GENERATING AN OPTIMIZED, DIVERSE POPULATION OF VARIANTS
Granted: February 10, 2011
Application Number:
20110034342
The disclosure relates to a method of generating a diverse set of variants to screen improved and novel properties within the variant population, a system for creating the diverse set of variants, and the variant peptides.
METHOD OF GENERATING AN OPTIMIZED, DIVERSE POPULATION OF VARIANTS
Granted: February 3, 2011
Application Number:
20110029468
The present disclosure relates to methods of rapidly and efficiently searching biologically-related data space to identify a population set maximally diverse and optimized for sets of desired properties. More specifically, the disclosure provides methods of identifying a diverse, evolutionary separated bio-molecules with desired properties from complex bio-molecule libraries. The disclosure additionally provides digital systems and software for performing these methods.
PRODUCTION OF FATTY ALCOHOLS WITH FATTY ALCOHOL FORMING ACYL-COA REDUCTASES (FAR)
Granted: January 6, 2011
Application Number:
20110000125
The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
BETA-GLUCOSIDASE VARIANT ENZYMES AND RELATED POLYNUCLEOTIDES
Granted: December 16, 2010
Application Number:
20100317059
The invention provides variants of the Azospirillum irakense CelA ?-glucosidase that have improve ?-glucosidase activity, particularly improved thermoactivity, compared to the wild type enzyme. The invention further provides related polynucleotides, vectors, host cell, and methods for making and using the variants.
GLUCOSE DEHYDROGENASE POLYPEPTIDES AND RELATED POLYNUCLEOTIDES
Granted: December 2, 2010
Application Number:
20100304459
The present invention is directed to glucose dehydrogenase (GDH) polypeptides that have enhanced GDH activity and/or thermostability relative to the backbone wild-type glucose dehydrogenase polypeptide. In addition, the present invention is directed to a polynucleotide that encodes for the GDH polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells…
ENGINEERED BIOSYNTHESIS OF FATTY ALCOHOLS
Granted: November 25, 2010
Application Number:
20100298612
The present disclosure provides a process for the production of long chain fatty alcohols by recombinant host cells expressing one or more heterologous carboxylic acid reductase enzymes useful for the conversion of fatty acids, and derivatives thereof, to long chain fatty alcohols.
TRANSAMINASE BIOCATALYSTS
Granted: November 11, 2010
Application Number:
20100285541
The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
ENDOGLUCANASES
Granted: October 21, 2010
Application Number:
20100267089
The present invention relates to variant endoglucanases and particularly endoglucanases having improved properties over wild-type endoglucanase.
INHA PROMOTERS
Granted: October 21, 2010
Application Number:
20100267088
The present invention relates to an isolated DNA sequence having promoter activity and to vectors comprising the DNA sequence. The promoters of the invention are referred to inhA promoters. The invention also relates to a method of producing a protein in a recombinant host cell wherein the host cell comprises a vector comprising an inhA promoter and a coding sequence encoding a protein of interest.
Alanine 2,3-aminomutases and related polynucleotides
Granted: September 30, 2010
Application Number:
20100248312
The present disclosure relates generally to polypeptides having improved alanine 2,3-aminomutase (AAM) activity, the polynucleotides encoding the AAM polypeptides, and expression vectors and host cells for expressing the AAM polypeptides.
CARBONIC ANHYDRASE POLYPEPTIDES AND USES THEREOF
Granted: August 19, 2010
Application Number:
20100209997
The present disclosure relates to recombinant carbonic anhydrase enzymes having improved properties as compared to a naturally-occurring wild type carbonic anhydrase and uses thereof for the sequestration of carbon dioxide as well as for the release of carbon dioxide from a composition comprising bicarbonate. Also provided are polynucleotides encoding the recombinant carbonic anhydrase enzymes and host cells capable of expressing the recombinant carbonic anhydrase enzymes.
TRANSAMINASE POLYPEPTIDES
Granted: August 19, 2010
Application Number:
20100209981
The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.
ENONE REDUCTASES
Granted: July 29, 2010
Application Number:
20100190218
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF 3-ARYL-3-HYDROXYPROPANAMINE FROM A 3-ARYL-3-KETOPROPANAMINE
Granted: July 8, 2010
Application Number:
20100173369
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the…
Recombinant Halohydrin Dehalogenase Polypeptides
Granted: July 8, 2010
Application Number:
20100173372
The present disclosure provides engineered halohydrin dehalogenase (HHDH) polypeptides having improved enzyme properties as compared to the wild-type HHDH enzyme HheC and other reference engineered HHDH polypeptides. Also provided are polynucleotides encoding the engineered HHDH enzymes, host cells capable of expressing the engineered HHDH enzymes, and methods of using the engineered HHDH enzymes to synthesize a variety of chiral compounds including chiral epoxides and chiral alcohols.
HALOHYDRIN DEHALOGENASES AND RELATED POLYNUCLEOTIDES
Granted: July 1, 2010
Application Number:
20100167345
The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.
