Beta-Glucosidase Variants
Granted: March 29, 2012
Application Number:
20120077216
The invention relates to recombinantly produced ?-Glucosidase Variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant ?-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target ?-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.
EXPRESSION CONSTRUCTS COMPRISING FUNGAL PROMOTERS
Granted: February 23, 2012
Application Number:
20120045793
The present invention provides promoters derived from a filamentous fungus. These promoters have application in the fields of molecular biology, microbiology, fungal genetics and production of biofuels and other products.
OLIGONUCLEOTIDE MEDIATED NUCLEIC ACID RECOMBINATION
Granted: February 16, 2012
Application Number:
20120040871
Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
ALANINE 2,3-AMINOMUTASES AND RELATED POLYNUCLEOTIDES
Granted: February 16, 2012
Application Number:
20120040437
The present disclosure relates generally to polypeptides having improved alanine 2,3-aminomutase (AAM) activity, the polynucleotides encoding the AAM polypeptides, and expression vectors and host cells for expressing the AAM polypeptides.
AMIDASES AND METHODS OF THEIR USE
Granted: January 26, 2012
Application Number:
20120021469
The disclosure relates to engineered amidase polypeptides and processes of using the polypeptides for chiral resolution of amino acid amide compounds. The disclosure further relates to the polynucleotides that encode the engineered amidase polypeptides and related vectors, host cells, and methods for making the engineered amidase polypeptides.
METHODS AND COMPOSITIONS FOR POLYPEPTIDE ENGINEERING
Granted: January 19, 2012
Application Number:
20120015820
Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
BETA-GLUCOSIDASE VARIANT ENZYMES AND RELATED POLYNUCLEOTIDES
Granted: January 19, 2012
Application Number:
20120015408
The invention provides variants of the Thermoanaerobacter brockii CglT beta-glucosidase that have improve beta-glucosidase activity compared to the wild type enzyme. The invention also provides polynucleotides that encode the variants, as well as methods of producing the variants, enzyme compositions comprising the variants, and methods for using the variants in industrial applications.
FATTY ALCOHOL FORMING ACYL REDUCTASES (FARS) AND METHODS OF USE THEREOF
Granted: January 12, 2012
Application Number:
20120009640
The present disclosure provides methods useful for producing fatty alcohol compositions from recombinant host cells. The disclosure further provides variant fatty acyl-CoA reductase (FAR) enzymes, polynucleotides encoding the variant FAR enzymes, and vectors and host cells comprising the same.
HIGHLY STABLE BETA-CLASS CARBONIC ANHYDRASES USEFUL IN CARBON CAPTURE SYSTEMS
Granted: January 12, 2012
Application Number:
20120009653
The present disclosure relates to ?-class carbonic anhydrase polypeptides having improved properties including increased thermostability and/or stability in the presence of amine compounds, ammonia, or carbonate ion. The present disclosure also provides formulations and uses of the polypeptides for accelerating the absorption of carbon dioxide from a gas stream into a solution as well as for the release of the absorbed carbon dioxide for further treatment and/or sequestering. Also…
HIGHLY STABLE BETA-CLASS CARBONIC ANHYDRASES USEFUL IN CARBON CAPTURE SYSTEMS
Granted: January 12, 2012
Application Number:
20120009652
The present disclosure relates to ?-class carbonic anhydrase polypeptides having improved properties including increased thermostability and/or stability in the presence of amine compounds, ammonia, or carbonate ion. The present disclosure also provides formulations and uses of the polypeptides for accelerating the absorption of carbon dioxide from a gas stream into a solution as well as for the release of the absorbed carbon dioxide for further treatment and/or sequestering. Also…
CHEMICALLY MODIFIED CARBONIC ANHYDRASES USEFUL IN CARBON CAPTURE SYSTEMS
Granted: January 12, 2012
Application Number:
20120009646
The present disclosure relates to chemically modified carbonic anhydrase polypeptides and soluble compositions, homogenous liquid formulations comprising them. The chemically modified carbonic anhydrase polypeptides have improved properties relative to the same carbonic anhydrase polypeptide that is not chemically modified including the improved properties of increased activity and/or stability in the presence of amine compounds, ammonia, or carbonate ion. The present disclosure also…
VARIANT ENDOGLUCANASES AND RELATED POLYNUCLEOTIDES
Granted: January 12, 2012
Application Number:
20120009631
The invention provides variants of the Clostridium thermocellum endoglucanase (CeIG) that have improved endoglucanase activity compared to the wild type enzyme. Also provided are related polynucleotides, compositions, vectors, host cells, and methods of use.
CELLOBIOHYDROLASE VARIANTS
Granted: January 5, 2012
Application Number:
20120003703
The invention relates to recombinant expression of variant forms of C1 CBH1a and homologs thereof, having improved thermostability, low-pH tolerance, specific activity and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.
Pentose Fermentation By a Recombinant Microorganism
Granted: December 1, 2011
Application Number:
20110294170
The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.
METHODS FOR IDENTIFYING SETS OF OLIGONUCLEOTIDES FOR USE IN AN IN VITRO RECOMBINATION PROCEDURE
Granted: October 20, 2011
Application Number:
20110257892
In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided.
METHODS, SYSTEMS, AND SOFTWARE FOR IDENTIFYING FUNCTIONAL BIOMOLECULES
Granted: October 20, 2011
Application Number:
20110257023
The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention…
KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF (R)-3-HYDROXYTHIOLANE
Granted: September 8, 2011
Application Number:
20110217754
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.
Compositions and Methods for Producing Stereoisomerically Pure Statins and Synthetic Intermediates Therefor
Granted: August 11, 2011
Application Number:
20110195465
The present disclosure provides ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, method of using the engineered ketoreductase enzymes to synthesize a variety of chirally pure compounds, and the chirally pure compounds prepared therewith.
Ketoreductases and Uses Thereof
Granted: July 7, 2011
Application Number:
20110165670
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF AZETIDINONE
Granted: June 30, 2011
Application Number:
20110159567
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
