Low-phosphate repressible promoter
Granted: June 6, 2017
Patent Number:
9670493
The present invention provides compositions and methods comprising a low-phosphate repressible promoter. In particular, the present invention provides a low-phosphate repressible promoter from E. coli.
Methods, systems, and software for identifying bio-molecules with interacting components
Granted: May 30, 2017
Patent Number:
9665694
The present invention provides methods for rapidly and efficiently searching biologically-related data space. More specifically, the present invention provides methods for identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The present invention also provides methods for modeling sequence-activity relationships, including but not limited to stepwise addition or…
Engineered biocatalysts useful for carbapenem synthesis
Granted: May 30, 2017
Patent Number:
9663771
The present disclosure provides engineered pNB esterase polypeptides useful for the synthesis of the carbapenem antibiotic, imipenem. The disclosure also provides polynucleotides encoding the engineered pNB esterases, host cells capable of expressing the engineered pNB esterases, and methods of using the engineered pNB esterases in the production of imipenem.
Engineered ketoreductase polypeptides
Granted: May 23, 2017
Patent Number:
9657320
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the…
Production of fatty alcohols from engineered microorganisms
Granted: May 16, 2017
Patent Number:
9650655
Recombinant microorganisms are provided which have been engineered to produce fatty alcohols. Also provided are recombinant microorganisms which comprise a heterologous polynucleotide encoding a fatty alcohol reductase enzyme and an introduced polynucleotide encoding a ?-ketoacyl acyl carrier protein synthase.
Biocatalysts for ezetimibe synthesis
Granted: May 9, 2017
Patent Number:
9644189
The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Synthesis of prazole compounds
Granted: April 25, 2017
Patent Number:
9631181
The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Biocatalysts and methods for the synthesis of substituted lactams
Granted: April 18, 2017
Patent Number:
9624478
The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.
Enone reductases
Granted: April 11, 2017
Patent Number:
9617568
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
Engineered transaminase polypeptides for industrial biocatalysis
Granted: April 11, 2017
Patent Number:
9617573
The present disclosure provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The disclosure also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.
Pentose fermentation by a recombinant microorganism
Granted: April 4, 2017
Patent Number:
9611515
The present invention provides methods and compositions suitable for use in the isomerization of xylose to xylulose, as well as methods and compositions suitable for use in the conversion of xylose to xylitol and xylulose, including nucleic acid constructs, recombinant fungal host cells, and related materials.
Engineered phenylalanine ammonia lyase polypeptides
Granted: April 4, 2017
Patent Number:
9611468
The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.
Endoglucanase 1B (EG1B) variants
Granted: April 4, 2017
Patent Number:
9611462
The present invention provides endoglucanase 1b (EG1b) variants suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce EG1b variants, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.
Biocatalytic process for preparing eslicarbazepine and analogs thereof
Granted: March 28, 2017
Patent Number:
9605290
The present disclosure relates to biocatalysts and its uses for the efficient preparation of eslicarbazepine, eslicarbazepine acetate, and analogs thereof.
Engineered tyrosine ammonia lyase
Granted: March 28, 2017
Patent Number:
9605252
The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.
Protein variant generation by region shuffling
Granted: March 14, 2017
Patent Number:
9593326
Region shuffling methods for efficiently introducing diversity and exploring sequence space are described. Libraries produced directly from these methods contain high fractions of protein variants harboring multiple beneficial mutations. Typically, the methods produce these variants efficiently without the need for sequencing beneficial mutants identified at intermediate stages of the process.
Ketoreductase polypeptides for the production of azetidinone
Granted: February 28, 2017
Patent Number:
9580694
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Transaminase biocatalysts
Granted: January 24, 2017
Patent Number:
9550982
The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Variant LovD polypeptides and their uses
Granted: January 17, 2017
Patent Number:
9546388
The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound.
Engineered biocatalysts useful for carbapenem synthesis
Granted: January 10, 2017
Patent Number:
9540622
The present disclosure provides engineered pNB esterase polypeptides useful for the synthesis of the carbapenem antibiotic, imipenem. The disclosure also provides polynucleotides encoding the engineered pNB esterases, host cells capable of expressing the engineered pNB esterases, and methods of using the engineered pNB esterases in the production of imipenem.
