Variant endoglucanases and related polynucleotides
Granted: October 11, 2016
Patent Number:
9464278
The invention provides variants of the Clostridium thermocellum endoglucanase (CelG) that have improved endoglucanase activity compared to the wild type enzyme. Also provided are related polynucleotides, compositions, vectors, host cells, and methods of use.
Production of saturated fatty alcohols from engineered microorganisms
Granted: September 20, 2016
Patent Number:
9447436
Recombinant bacterial microorganisms are provided which comprise heterologous fatty acyl reductases (“FAR”) polypeptides wherein said microorganisms have been engineered to produce increased amounts of saturated fatty alcohols and methods of making saturated fatty alcohols using the recombinant bacterial microorganisms.
Transaminase reactions
Granted: September 6, 2016
Patent Number:
9434968
The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.
Fungal proteases
Granted: September 6, 2016
Patent Number:
9434919
The present invention provides fungal proteases and improved fungal strains that are deficient in protease production.
Synthesis of prazole compounds
Granted: August 23, 2016
Patent Number:
9422534
The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Biocatalysts for the preparation of hydroxy substituted carbamates
Granted: August 23, 2016
Patent Number:
9422531
The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
Ketoreductase polypeptides
Granted: August 23, 2016
Patent Number:
9422530
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the…
Ketoreductase-mediated stereoselective route to alpha chloroalcohols
Granted: August 2, 2016
Patent Number:
9404092
The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ?-chloroalcohols from ?-chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides.
Lov-D acyltransferase mediated acylation
Granted: July 26, 2016
Patent Number:
9399785
Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.
Ketoreductase polypeptides for the production of ()-3-hydroxythiolane
Granted: July 19, 2016
Patent Number:
9394552
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.
Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system
Granted: July 19, 2016
Patent Number:
9394551
The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.
Enone reductases
Granted: July 12, 2016
Patent Number:
9388438
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs
Granted: July 12, 2016
Patent Number:
9388395
The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.
Biocatalysts for Ezetimibe synthesis
Granted: July 12, 2016
Patent Number:
9388391
The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Ketoreductase polypeptides for the production of azetidinone
Granted: July 5, 2016
Patent Number:
9382519
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Biocatalytic process for preparing eslicarbazepine and analogs thereof
Granted: June 14, 2016
Patent Number:
9365878
The present disclosure relates to biocatalysts and its uses for the efficient preparation of eslicarbazepine, eslicarbazepine acetate, and analogs thereof.
Biocatalysts and methods for the synthesis of armodafinil
Granted: June 14, 2016
Patent Number:
9365835
The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (?)-2-[(R)-(diphenylmethyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid,…
Transaminase biocatalysts
Granted: May 31, 2016
Patent Number:
9353355
The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Ketoreductase-mediated stereoselective route to alpha chloroalcohols
Granted: March 29, 2016
Patent Number:
9296992
The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ?-chloroalcohols from ?-chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides.
Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
Granted: March 29, 2016
Patent Number:
9296993
The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
