Recombinant ?-glucosidase variants for production of soluble sugars from cellulosic biomass
Granted: April 1, 2014
Patent Number:
8685690
The invention relates to recombinant expression of a variant form of a fungal C1 strain ?-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the ?-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant ?-glucosidase variant protein,…
Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
Granted: March 18, 2014
Patent Number:
8673607
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the…
Pentose fermentation by a recombinant microorganism
Granted: March 4, 2014
Patent Number:
8663962
The present invention provides methods and compositions suitable for use in the conversion of xylose to xylitol and xylulose, including nucleic acid constructs, recombinant fungal host cells, and related materials.
Endoglucanases
Granted: March 4, 2014
Patent Number:
8663960
The present invention relates to variant endoglucanases and particularly endoglucanases having improved properties over wild-type endoglucanase.
Recombinant ?-glucosidase variants
Granted: February 18, 2014
Patent Number:
8652813
The present invention provides compositions and methods for the expression of recombinant ?-glucosidase variants, as well as their use in the production of fermentable sugars from cellulosic biomass.
Polynucleotides encoding ketoreductases for producing stereoisomerically pure statins and synthetic intermediates therefor
Granted: December 31, 2013
Patent Number:
8617864
The present disclosure provides ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, method of using the engineered ketoreductase enzymes to synthesize a variety of chirally pure compounds, and the chirally pure compounds prepared therewith.
Ketoreductase polypeptides for the production of (S,E)-methyl 2-(3-(3-(2-(7-chloroquinolin-2-yl)vinyl)phenyl)-3-hydroxypropyl)benzoate
Granted: December 31, 2013
Patent Number:
8617853
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Nitrilase biocatalysts
Granted: December 24, 2013
Patent Number:
8614081
The present disclosure relates to polypeptides having nitrilase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Methods of populating data structures for use in evolutionary simulations
Granted: November 19, 2013
Patent Number:
8589085
In particular, this invention provides novel methods of populating data structures for use in evolutionary modeling. In particular, this invention provides methods of populating a data structure with a plurality of character strings. The methods involve encoding two or more biological molecules into character strings to provide a collection of two or more different initial character strings; selecting at least two substrings from the pool of character strings; concatenating the…
Recombinant halohydrin dehalogenase polypeptides
Granted: November 12, 2013
Patent Number:
8580555
The present disclosure provides engineered halohydrin dehalogenase (HHDH) polypeptides having improved enzyme properties as compared to the wild-type HHDH enzyme HheC and other reference engineered HHDH polypeptides. Also provided are polynucleotides encoding the engineered HHDH enzymes, host cells capable of expressing the engineered HHDH enzymes, and methods of using the engineered HHDH enzymes to synthesize a variety of chiral compounds including chiral epoxides and chiral alcohols.
Production of fatty alcohols with fatty alcohol forming acyl-CoA reductases (FAR)
Granted: November 5, 2013
Patent Number:
8574877
The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
Biocatalytic processes for the preparation of stereomerically pure fused bicyclic proline compounds
Granted: November 5, 2013
Patent Number:
8574876
The present disclosure provides substantially enantiomerically pure heterobicyclic compounds of the following structural formulas, wherein A, M, M?, and R5 are as described herein, and to biocatalytic processes for their preparation, and to the enzymes used in those processes.
Chemically modified carbonic anhydrases useful in carbon capture systems
Granted: October 29, 2013
Patent Number:
8569031
The present disclosure relates to chemically modified carbonic anhydrase polypeptides and soluble compositions, homogenous liquid formulations comprising them. The chemically modified carbonic anhydrase polypeptides have improved properties relative to the same carbonic anhydrase polypeptide that is not chemically modified including the improved properties of increased activity and/or stability in the presence of amine compounds, ammonia, or carbonate ion. The present disclosure also…
Penicillin-G acylases
Granted: October 29, 2013
Patent Number:
8569013
The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
Halohydrin dehalogenases and related polynucleotides
Granted: September 17, 2013
Patent Number:
8535910
The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.
Method of converting a biomass substrate to a fermentable sugar using a beta-glucosidase variant enzyme
Granted: September 10, 2013
Patent Number:
8530201
The invention provides variants of the Azospirillum irakense CelA ?-glucosidase that have improve ?-glucosidase activity, particularly improved thermoactivity, compared to the wild type enzyme. The invention further provides related polynucleotides, vectors, host cell, and methods for making and using the variants.
Highly stable beta-class carbonic anhydrases useful in carbon capture systems
Granted: August 20, 2013
Patent Number:
8512989
The present disclosure relates to ?-class carbonic anhydrase polypeptides having improved properties including increased thermostability and/or stability in the presence of amine compounds, ammonia, or carbonate ion. The present disclosure also provides formulations and uses of the polypeptides for accelerating the absorption of carbon dioxide from a gas stream into a solution as well as for the release of the absorbed carbon dioxide for further treatment and/or sequestering. Also…
Methods of using engineered ketoreductase polypeptides for the stereoselective reduction of acetophenones
Granted: August 20, 2013
Patent Number:
8512973
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Method of generating an optimized, diverse population of variants
Granted: August 6, 2013
Patent Number:
8504498
The present disclosure relates to methods of rapidly and efficiently searching biologically-related data space to identify a population set maximally diverse and optimized for sets of desired properties. More specifically, the disclosure provides methods of identifying a diverse, evolutionary separated bio-molecules with desired properties from complex bio-molecule libraries. The disclosure additionally provides digital systems and software for performing these methods.
Transaminase polypeptides
Granted: June 25, 2013
Patent Number:
8470564
The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.
