Enone reductases
Granted: November 11, 2014
Patent Number:
8883475
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
Production of fatty alcohols with fatty alcohol forming acyl-CoA reductases (FAR)
Granted: November 11, 2014
Patent Number:
8883467
The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
Polynucleotides encoding engineered ketoreductase polypeptides
Granted: November 4, 2014
Patent Number:
8877475
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the…
GH61 glycoside hydrolase protein variants and cofactors that enhance GH61 activity
Granted: November 4, 2014
Patent Number:
8877474
The present invention provides various GH61 protein variants comprising various amino acid substitutions. The GH61 protein variants have an improved ability to synergize with cellulase enzymes, thereby increasing the yield of fermentable sugars obtained by saccharification of biomass. In some embodiments, sugars obtained from saccharification are fermented to produce numerous end-products, including but not limited to alcohol.
Stereomerically pure fused bicyclic proline compounds useful for preparing hepatitis C protease inhibitors
Granted: October 14, 2014
Patent Number:
8859784
The present disclosure provides substantially enantiomerically pure heterobicyclic compounds of the following structural formulas, wherein A, M, M?, and R5 are as described herein, and to biocatalytic processes for their preparation, and to the enzymes used in those processes.
Ketoreductase polypeptides for the reduction of acetophenones
Granted: October 7, 2014
Patent Number:
8852909
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Biocatalysts and methods for the synthesis of ()-3-(1-aminoethyl)-phenol
Granted: October 7, 2014
Patent Number:
8852900
The present disclosure provides engineered transaminase polypeptides having improved properties as compared to naturally occurring transaminases including the ability of converting the substrate, 3?-hydroxyacetophenone to (S)-3-(1-aminoethyl)-phenol in enantiomeric excess and high percentage conversion. Also provided are polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases…
Methods, systems, and software for identifying functional biomolecules
Granted: September 30, 2014
Patent Number:
8849575
The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention…
Variant LOVD polypeptides and their uses
Granted: September 30, 2014
Patent Number:
8846362
The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound.
Polypeptides for a ketoreductase-mediated stereoselective route to alpha chloroalcohols
Granted: August 5, 2014
Patent Number:
8796002
The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ? chloroalcohols from ? chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides.
Variant CBH2 cellulases and related polynucleotides
Granted: July 22, 2014
Patent Number:
8785170
The invention provides variants of a Streptomyces sp. CBH2 that have improved properties compared to the wild type enzyme and methods of using the variants in the hydrolysis of substrates comprising cellulose.
Pentose fermentation by a recombinant microorganism
Granted: July 15, 2014
Patent Number:
8778652
The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.
?-Glucosidase variants
Granted: July 8, 2014
Patent Number:
8772010
The invention relates to recombinantly produced ?-Glucosidase Variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant ?-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target ?-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.
Method of generating an optimized, diverse population of variants
Granted: July 1, 2014
Patent Number:
8768871
The disclosure relates to a method of generating a diverse set of variants to screen improved and novel properties within the variant population, a system for creating the diverse set of variants, and the variant peptides.
Methods, systems, and software for identifying functional biomolecules
Granted: June 24, 2014
Patent Number:
8762066
The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention…
Cellobiohydrolase variants
Granted: June 24, 2014
Patent Number:
8759064
The invention relates to recombinant expression of variant forms of C1 CBH1a and homologs thereof, having improved thermostability, low-pH tolerance, specific activity and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.
Ketoreductase polypeptides for the reduction of acetophenones
Granted: June 10, 2014
Patent Number:
8748143
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Production of geranyl diphosphate
Granted: May 6, 2014
Patent Number:
8715962
The invention relates to recombinant geranyl diphosphate (GPP) synthases, genes encoding the synthases, vectors and host cells comprising the same. More particularly, the invention relates to a recombinant GPP synthase, which preferentially facilitates the production of GPP from its isoprenoid precursors and incorporating a recombinant GPP synthase into one or more terpene production pathways of a host organism.
Beta-glucosidase variant enzymes and related polynucleotides
Granted: May 6, 2014
Patent Number:
8715996
The invention provides variants of the Thermoanaerobacter brockii CglT beta-glucosidase that have improve beta-glucosidase activity compared to the wild type enzyme. The invention also provides polynucleotides that encode the variants, as well as methods of producing the variants, enzyme compositions comprising the variants, and methods for using the variants in industrial applications.
Method for producing an alcohol using ?-glucosidase variant enzymes
Granted: May 6, 2014
Patent Number:
8715975
The invention provides variants of the Azospirillum irakense CelA ?-glucosidase that have improve ?-glucosidase activity, particularly improved thermoactivity, compared to the wild type enzyme. The invention further provides related polynucleotides, vectors, host cell, and methods for making and using the variants.
