Systems and methods for tracking and providing workflow information
Granted: November 10, 2020
Patent Number:
10832199
A tangible computer-readable storage device storing computer-executable program instructions that generate a user interface for displaying workflow information associated with a tissue specimen in a pathology laboratory. The program instructions may perform a method including displaying a virtual laboratory component representing a physical pathology laboratory having one or more laboratory stations for processing the tissue specimen according to a workflow, and displaying a specimen…
Temporary storage of sample portions for artefact-preventing fluid processing
Granted: November 10, 2020
Patent Number:
10830739
In a method for processing successive fluidic sample portions provided by a sample source, sample reception volumes are filled successively temporarily with at least a respective one of the sample sections, and the sample sections are emptied successively out of the sample reception volumes in such a way, that, while emptying, it is avoided to bring two respective ones of the sample sections, which have not left the sample source directly adjacent to one another, in contact with one…
Method of oligonucleotide analysis
Granted: September 22, 2020
Patent Number:
10781484
Described herein, among other things, is a method of estimating efficiency of an oligonucleotide synthesis reaction. In some embodiments, the method comprises subjecting the products of one or more oligonucleotide synthesis reactions to LC-MS to produce a series of mass spectra, analyzing the mass spectra, and estimating the overall efficiency of an oligonucleotide synthesis reaction and/or the efficiency of addition of one or more of G, A, T or C individually in an oligonucleotide…
Components with an atomic layer deposition coating and methods of producing the same
Granted: September 8, 2020
Patent Number:
10767259
Liquid chromatography and gas chromatography components with a conformal protective coating on internal surfaces thereof and methods for producing the same are provided. The conformal protective coating is formed via an atomic layer deposition (ALD) process. The components and methods of the present disclosure find use in providing protective coatings for liquid chromatography and gas chromatography components that are compatible with bioanalytical separations and sensitive trace…
High specificity genome editing using chemically modified guide RNAs
Granted: September 8, 2020
Patent Number:
10767175
The present invention relates to guide RNAs having chemical modifications and their use in CRISPR-Cas systems. The chemically modified guide RNAs have enhanced specificity for target polynucleotide sequences. The present invention also relates to methods of using chemically modified guide RNAs for cleaving or nicking polynucleotides, and for high specificity genome editing.
Method and apparatus for transferring a tissue section using a thermoplastic material
Granted: September 1, 2020
Patent Number:
10760999
Methods and apparatus are provided for transfer of a tissue section from a first location to a second location. Thermoplastic material is applied to at least one pin of a tissue section transferring apparatus. The thermoplastic material contacts a tissue section while at least partially melted, and when it cools, substantially solid thermoplastic material holds the tissue section to the at least one pin. A tissue section transferring apparatus comprises a heater block, a heat source, and…
Conical heater assembly for a gas chromatography column
Granted: September 1, 2020
Patent Number:
10764964
A heating apparatus for a gas chromatography column is described. The GC column heating apparatus includes a conical heater assembly comprising a heating element between an inner layer and an outer layer. The apparatus can also include optionally an outer cowl and/or an inner cowl surrounding the conical heater assembly. The conical heater assembly rapidly increases the temperature of the column by conductive heating. The selection of shape (in particular, the cone angle of a conical…
Systems and methods for transferring liquids
Granted: September 1, 2020
Patent Number:
10761104
One or more liquids are transferred from a source array to one or more remotely positioned destination sites such as chambers by utilizing one or more movable transfer elements, such as contact pins or capillaries. The source array may include a predetermined organization of addresses at which materials are positioned. One or more materials may be selected for transfer. Based on the selection, one or more addresses may be accessed by the transfer element(s). The addresses may correspond…
Artifact compensation due to different properties of fluid accommodation volumes in sample separation apparatus
Granted: September 1, 2020
Patent Number:
10761071
A control device for controlling at least part of a sample separation apparatus for separating a fluidic sample, the sample separation apparatus including at least two fluid accommodation volumes having different flow through properties and each being configured for temporarily accommodating fluidic sample, wherein the control device is configured for controlling operation of at least part of the sample separation apparatus for at least partially compensating sample separation artifacts…
Systems and methods for automated alignment, calibration and standardization of electrophoresis data
Granted: September 1, 2020
Patent Number:
10761054
Systems and methods are provided for improving the analysis of analytes by using electrophoresis apparatus. Exemplary methods provide an increase in the yield of useful results, e.g., quantity and quality of useable data, in automated peak detection, in connection with an electrophoretic separation, e.g., capillary electrophoresis. In various embodiments, the system virtualizes the raw data, transforming the migration time into virtual units thereby allowing the visual comparison of…
Method and apparatus for characterizing laser gain chips
Granted: August 4, 2020
Patent Number:
10732105
A system adapted for characterizing gain chips and a method for characterizing gain chips are disclosed. The system includes a probe light source that generates an output light signal characterized by a wavelength that can be varied in response to a wavelength control signal and a mounting stage adapted for receiving a gain chip characterized by a waveguide having a reflective face on a first surface of the gain chip and a transparent face on a second surface of the gain chip. The system…
Combinatorial analysis and repair
Granted: July 28, 2020
Patent Number:
10722562
A method for the repair of a unit, by specific diagnosis of the undesired state, and its appropriate repair, using said specific diagnosis as a means to repair in an appropriate way said unit. The diagnosis and repair processes may involve chemical, physical, or mechanical means. The units being diagnosed and repaired include live matter (e.g. human beings, animals, plants) as well as non-live matter (e.g. buildings, electronic equipment, polymer materials).
Combined histological stain
Granted: July 21, 2020
Patent Number:
10718777
The present invention relates to methods of visualizing targets in histological samples, e.g. biopsy samples, wherein the methods comprise staining of the sample with (i) one or more target specific immunochemical stains, and (ii) a histological stain for specific tissue components e.g. iron, mucins glycogen, amyloid, nucleic acids, etc., e.g. hematoxilyn and/or eosin stains or the like, that is used to enhance contrast in the microscopic image of a tissue sample, highlight morphologic…
Immunohistochemistry scoring methods and compositions
Granted: July 21, 2020
Patent Number:
10718773
Aspects of the present disclosure provide methods for determining the eligibility of a subject having a malignancy for treatment with an anti-PD therapeutic agent. In certain embodiments, the method includes determining, by immunohistochemistry, the number of PD-L1 positive malignant cells in a tumor tissue section as well as the number of infiltrating non-malignant cells and/or non-malignant cells of the stromal interface area. The infiltrating non-malignant cells and/or non-malignant…
Liquid chromatography columns with structured walls
Granted: July 21, 2020
Patent Number:
10718743
A liquid chromatography (LC) column includes a wall having a length along a central axis from the inlet end to the outlet end, the wall enclosing a column interior and having a column radius relative to the central axis, the wall comprising a structured portion configured such that the column radius varies along the length; and a plurality of particles packed in the column interior, wherein at least some of the particles are in contact with the structured portion.
Method for making an asymmetrically-tagged sequencing library
Granted: July 14, 2020
Patent Number:
10711269
A method for making an asymmetrically-tagged sequencing library is provided. In some embodiments, the method may comprise: obtaining a symmetrically-tagged library of cDNA or genomic DNA fragments, hybridizing a tailed first primer to the 3? sequence tag of the library and extending the same to produce primer extension products, and amplifying the primer extension products using a pair of tailed primers to produce asymmetrically-tagged library.
Chromatograph
Granted: July 7, 2020
Patent Number:
D889679
Hairpin-mediated amplification method
Granted: June 30, 2020
Patent Number:
10697006
In some embodiments, the amplification method may comprise producing a reaction mix comprising: a nucleic acid sample, a polymerase, nucleotides, a forward primer that hybridizes to a sequence in the bottom strand of a fragment in the sample, and a reverse primer. The reverse primer has a hairpin structure comprising a loop, a stem and a 3? overhang of at least 8 nucleotides, wherein the 3? overhang hybridizes to a sequence in the top strand of the fragment. Subjecting the reaction mix…
Device and method of a supercritical fluid system for detecting analytes using a low pressure detector
Granted: June 30, 2020
Patent Number:
10695692
A device is provided in a supercritical fluid system, which uses a mobile phase output by a separation device, the mobile phase volumetrically expanding as it decompresses. The device includes a passive splitter and a shuttle valve. The passive splitter is configured to receive the mobile phase and to split the mobile phase into a primary flow stream and a split flow stream, where the primary flow stream is directed to a pressure maintenance device. The passive splitter is further…
Pairing code directed assembly
Granted: June 23, 2020
Patent Number:
10689680
Methods for making a synthetic nucleic acid which comprise: (a) identifying a conflicting nucleotide sequence in a target sequence; (b) inserting a masking sequence into the conflicting sequence to produce a disrupted target sequence, wherein: (i) the masking sequence comprises recognition sites for one or more Type IIS restriction endonucleases; and (ii) digestion of said disrupted target sequence by said one or more Type IIS restriction endonucleases followed by re-ligation…
