Pairing code directed assembly
Granted: June 23, 2020
Patent Number:
10689680
Methods for making a synthetic nucleic acid which comprise: (a) identifying a conflicting nucleotide sequence in a target sequence; (b) inserting a masking sequence into the conflicting sequence to produce a disrupted target sequence, wherein: (i) the masking sequence comprises recognition sites for one or more Type IIS restriction endonucleases; and (ii) digestion of said disrupted target sequence by said one or more Type IIS restriction endonucleases followed by re-ligation…
Preparation of tissue sections using fluorescence-based detection
Granted: June 16, 2020
Patent Number:
10684199
The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or…
Hybridization compositions and methods using formamide
Granted: May 26, 2020
Patent Number:
10662465
The invention provides methods and compositions for hybridizing at least one molecule to a target. The composition comprises at least one nucleic acid sequence, formamide, and a hybridization solution, wherein the concentration of formamide is less than or equal to 25%.
Molecular fabrication
Granted: May 26, 2020
Patent Number:
10662424
Provided herein is a method for fabricating transformable or transfectable molecules that includes an assembly reaction containing a variety of pre-made cassettes possessing ends that hybridize to one another, transforming or transfecting said molecules into a desired host cell and then selecting a transformed/transfected host cell containing plasmid molecules composed of said the cassettes. A kit for performing the method is also provided.
Synthetic silica as packing material in supported liquid extraction
Granted: May 26, 2020
Patent Number:
10661250
In embodiments, a packing material for supported liquid extraction has a sorbent media that includes synthetic silica particles. In embodiments, the synthetic silica particles can have physical properties relating to one or more of particle surface area, shape, size, or porosity. In one embodiment, synthetic silica particles have a surface area less than about 30 m2/g. In another embodiment, the synthetic silica particles have an approximately uniform particle shape. In further examples,…
Chromatograph
Granted: May 26, 2020
Patent Number:
D885216
Three-dimensional infrared imaging of surfaces utilizing laser displacement sensors
Granted: May 19, 2020
Patent Number:
10656402
A scanning apparatus having a stage adapted to hold a specimen to be imaged and to move the specimen in a first direction, and a light source that includes a tunable laser that generates a light beam having an illumination wavelength that varies as a function of an input signal is disclosed. The apparatus includes an imaging system having a scanning assembly that includes a focusing lens that focuses the light beam to a measurement point on the specimen, a first mirror that moves in a…
Hybridization compositions and methods
Granted: May 19, 2020
Patent Number:
10655177
The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, utilize a of cyclic and/or non-cyclic solvent that is non-toxic and may eliminate or reduce the amount of formamide in the hybridization composition.
Portable chromatograph
Granted: May 19, 2020
Patent Number:
D884524
Method for evaluation of target in histological sample
Granted: May 12, 2020
Patent Number:
10648970
The present invention lies in the field of visualization and quantification of immobilized targets in samples using immunochemical means. In particular, the invention relates to a method and reagents for detection, visualization and quantification of a molecular target in immunostained histological samples using particular compositions of the target specific binding agent. Methods and compositions of the invention are suitable for any assay that uses a target visualization system in…
Experimental chamber with computer-controlled display wall
Granted: May 5, 2020
Patent Number:
10642354
An environmental chamber having an interior compartment, an augmented display, and a controller is disclosed. The interior compartment is adapted for isolating an experimental setup from an environment external to the interior compartment. The augmented display is positioned to allow a user in the external environment to view the interior compartment and an image generated on the augmented display. The controller generates the image. The image includes information about a component…
Sample injection with fluidic connection between fluid drive unit and sample accommodation volume
Granted: May 5, 2020
Patent Number:
10641746
An injector, for injecting a fluidic sample into a flow path between a fluid drive and a sample separation unit, includes a sample accommodation volume, a sample drive, and a fluidic valve switchable to selectively couple the volume with the flow path or decouple the volume from the flow path. In an injection switching state, the fluid drive, the separation unit and the sample drive are coupled by the valve so that fluid driven by the sample drive and flowing from the volume to the…
Assay methods and compositions for detecting contamination of nucleic acid identifiers
Granted: April 28, 2020
Patent Number:
10633651
The present invention relates to nucleic acid samples for massively parallel sequencing. More particularly, the present invention relates to assay methods, compositions and kits for detecting contamination of nucleic acid identifiers such as sample barcodes.
Method for attaching a counter sequence to a nucleic acid sample
Granted: April 21, 2020
Patent Number:
10626441
Described herein is a method for adding a counter sequence to the individual polynucleotide molecules of an initial nucleic acid sample. After addition of the counter sequence, the sample may be amplified and the number of initial target molecules in the sample can be estimated by counting the number of counter sequences associated with the amplified target molecules.
Methods of liquid chromatography for anionic compounds
Granted: April 14, 2020
Patent Number:
10620171
The present disclosure generally relates to improved methods for separating and analyzing compounds by liquid chromatography, particularly when coupled with mass spectrometry. The methods include the addition of an additive to a mobile phase carrying a sample. The mobile phase additive may be effective for improving peak shapes of targeted analytes in acquired data, and/or eliminating or at least reducing ion suppression. The methods are particularly suited for anionic compounds such as…
Functionalized particles having modified phases
Granted: April 14, 2020
Patent Number:
10618920
Modified silica particles are provided. Aspects of the particles include an outer layer that is composed of organically-modified silica comprising a siloxane-linked hydrophilic group, such as a charged functional group or a polar neutral functional group. The modified silica particles can form the basis of a variety of chromatography support materials. Also provided are methods of preparing the subject particles. Aspects of the methods include contacting silica particles with water, an…
Methods of in vivo engineering of large sequences using multiple CRISPR/cas selections of recombineering events
Granted: April 7, 2020
Patent Number:
10612043
The present invention provides a method for making a large nucleic acid having a defined sequence in vivo. The method combines recombineering techniques with a CRISPR/Cas system to permit multiple insertions of defined sequences into a target nucleic acid at one time, double stranded cleavage of target nucleic acids in which the defined sequences were not successfully inserted, and selection of successful recombinant cells. The method further includes repeating the process one or more…
Electron multiplier for mass spectrometer
Granted: April 7, 2020
Patent Number:
10615019
A secondary electron multiplier includes: a conversion dynode for emitting a secondary electron in response to an incident ion; a plurality of dynodes configured to have multi-stages from second to final stages for receiving the secondary electron; and a first voltage applying device for applying a first negative voltage to the conversion dynode and sequentially dividing the first negative voltage to apply to each of the second-stage and subsequent dynodes, wherein the secondary electron…
Scoring methods for anti-PD therapy eligibility and compositions for performing same
Granted: April 7, 2020
Patent Number:
10613092
Aspects of the present disclosure provide methods for determining the eligibility of a subject having a malignancy for treatment with an anti-PD therapeutic agent based on a Combined Positive Score (CPS) for a tumor tissue sample from the subject. Compositions and kits or performing the disclosed methods are also provided.
Deparaffinization of tissue by electric field generation and ionization
Granted: April 7, 2020
Patent Number:
10613005
Paraffin-embedded tissue, which may be disposed on a solid substrate, is prepared by a dry technique that removes paraffin from tissue without adding any liquid to the tissue, thereby rendering the tissue substantially free of paraffin. The dry technique may entail applying heat energy to the tissue effective to melt the paraffin and thereby render it flowable, and applying an electric field. The electric field is effective to impart electrical charge to the paraffin and to move the…
