Scoring methods for anti-PD therapy eligibility and compositions for performing same
Granted: April 7, 2020
Patent Number:
10613092
Aspects of the present disclosure provide methods for determining the eligibility of a subject having a malignancy for treatment with an anti-PD therapeutic agent based on a Combined Positive Score (CPS) for a tumor tissue sample from the subject. Compositions and kits or performing the disclosed methods are also provided.
Protected monomer and method of final deprotection for RNA synthesis
Granted: March 24, 2020
Patent Number:
10597421
A method of deprotecting a solid support bound polynucleotide comprising at least one 2?-protected ribonucleotide in which a step of contacting the polynucleotide with a composition comprising a diamine is performed under conditions sufficient to deprotect and cleave the polynucleotide which remains retained on the solid support.
Sealing moving with piston in a high-pressure pump
Granted: March 17, 2020
Patent Number:
10591446
A piston member for a high-pressure pump for pumping fluid in a sample separation apparatus, wherein the piston member comprises a piston configured for being mountable to reciprocate in a piston chamber for displacing fluid, and a sealing for sealing between the piston member and the piston chamber when the piston member is mounted in the piston chamber to reciprocate, wherein the sealing is mounted on the piston so as to reciprocate together with the piston.
Polymerase idling method for single molecule DNA sequencing
Granted: March 17, 2020
Patent Number:
10590479
A method for sequencing a nucleic acid is provided. In certain embodiments the method comprises obtaining a duplex comprising a nucleic acid and a primer, wherein the primer has a nuclease resistant 3? end, combining the duplex with a chain terminator nucleotide and a proof-reading polymerase to produce a reaction in which the polymerase idles on the added chain terminator nucleotide, identifying the chain terminator nucleotide added to the end of the primer; and adding a…
In-situ conditioning in mass spectrometry systems
Granted: March 3, 2020
Patent Number:
10580632
In a mass spectrometer or gas chromatograph/mass spectrometer system, one or more different conditioning gases are added to condition or modify one or more surfaces or regions of the ion source. The conditioning gas(es) may be added directly into the ion source. The conditioning gas may be added off-line, when the mass spectrometer is not analyzing a sample.
Ion pump shield
Granted: March 3, 2020
Patent Number:
10580629
An ion pump with a housing enclosing an interior, a gas inlet having a through-hole extending into the interior of the ion pump, at least one cathode, at least one anode positioned in proximity to the at least one cathode, a magnet disposed on an opposite side of the at least one cathode from the anode, and a blocking shield disposed between the gas inlet and the at least one cathode. The blocking shield is electrically connected to the at least one anode. An associated method installs…
Method for fragmenting genomic DNA using CAS9
Granted: March 3, 2020
Patent Number:
10577644
A method for fragmenting a genome is provided. In certain embodiments, the method comprises: (a) combining a genomic sample containing genomic DNA with a plurality of Cas9-gRNA complexes, wherein the Cas9-gRNA complexes comprise a Cas9 protein and a set of at least 10 Cas9-associated guide RNAs that are complementary to different, pre-defined, sites in a genome, to produce a reaction mixture; and (b) incubating the reaction mixture to produce at least 5 fragments of the genomic DNA. Also…
Compositions and methods for analytical sample preparation
Granted: February 18, 2020
Patent Number:
10564076
Aspects of the present disclosure include a system for analytical sample preparation. In some embodiments, the system includes a container having disposed therein an analytical sample treatment composition including ?-cyclodextrin and/or an ?-cyclodextrin co-polymer. Also provided is a method of reducing matrix effects in an analytical sample. In some embodiments, the method includes: contacting a sample including a matrix-interfering agent and an analyte with a cyclodextrin composition…
Composition and method for improving signal-to-noise in hybridization to oligonucleotide arrays
Granted: February 4, 2020
Patent Number:
10550425
Provided herein, among other things, are compositions for improving signal-to-noise in hybridization to oligonucleotide arrays. In some embodiments, the composition may comprise a population of indexing oligonucleotides of formula X-Y, where the sequences of region X hybridize to an array and the sequences of region Y hybridize to distinct sites within a genomic region, transcript, set of transcripts, or complement of the same. Use of these oligonucleotides increases signal, reduces…
System and method of data-dependent acquisition by mass spectrometry
Granted: February 4, 2020
Patent Number:
10553412
Systems, computer-readable media, and methods using mass spectrometry to analyze a sample are provided. For example, a method includes: acquiring a precursor ion spectrum; analyzing the precursor ion spectrum to identify precursor ions that preliminarily match one or more peptides that each belong to at least one protein of interest for the analysis; selecting each of the identified precursor ions in an order according to a ranking protocol for maximizing the number of proteins that are…
Dielectric coated ion transfer device for mass spectrometry
Granted: January 28, 2020
Patent Number:
10546738
An ion transfer device includes a tube, a resistive layer on an inside surface of the tube, and a dielectric layer on the resistive layer. The device defines a conduit providing a transfer path for gas and ions. The conduit is surrounded by the dielectric layer. The dielectric layer protects the resistive layer from the chemical environment in the conduit, while being thin enough to allow charges to pass through the dielectric layer and be dissipated by the resistive layer.
Method and apparatus for automating contact between an ATR crystal and a specimen
Granted: January 21, 2020
Patent Number:
10539504
An ATR scanning system and a method for positioning a specimen against the reflective surface of an ATR objective are disclosed. The scanning system includes an ATR objective, a controller, a stage, and a height profiler. The controller forms an image of the reflective surface. The stage moves a specimen in a direction toward the reflective surface at a speed determined by the controller. The height profiler measures a minimum distance between the specimen and the reflective surface as…
On-array ligation assembly
Granted: January 21, 2020
Patent Number:
10538796
Provided herein, among other things, is a method for producing a ligation product on a support. In some embodiments, the method may comprise hybridizing a first double-stranded oligonucleotides and a second double-stranded oligonucleotide to a substrate comprising surface-tethered oligonucleotides and ligating the distal ends of the first and second double-stranded oligonucleotides together, thereby producing a first ligation product that is tethered to the support at both ends.
Methods and compositions for blocking off-target nucleic acids from cleavage by CRISPR proteins
Granted: January 21, 2020
Patent Number:
10538750
This invention relates to reagents and methods for increasing specificity and efficiency of genome editing by CRISPR associated (Cas) protein systems, more particularly by Cas:guide RNA complexes, by blocking off-target nucleic acids from cleavage by Cas:guide RNA complexes.
Chromogenic peroxidase substrates
Granted: January 7, 2020
Patent Number:
10526646
Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.
Compounds and methods for CRISPR/Cas-based genome editing by homologous recombination
Granted: January 7, 2020
Patent Number:
10526590
The present invention relates to guide RNAs comprising adaptor segments having one or more modifications, and their use in homologous recombination by CRISPR:Cas systems. The modified adaptor segments are resistant to degradation by RNaseH. The present invention also relates to a dual guide RNA strategy in which a first guide RNA directs a Cas enzyme to make a double-strand break at a first target sequence, and a second guide RNA comprises an adaptor segment attached to a donor…
Creating and harvesting surface-bound emulsion
Granted: December 31, 2019
Patent Number:
10518241
Provided is an emulsion comprising: (a) droplets that contain a single polymeric compound or a pre-defined mixture thereof, and (b) an immiscible liquid, wherein: (i) each of the droplets comprises multiple molecules of the compound(s) contained therein; and the droplets do not contain monomeric precursors for the polymeric compound. A method for making the emulsion is also provided.
Mass spectrometry using plasma ion source
Granted: December 17, 2019
Patent Number:
10510522
To correct spectral interference due to a divalent ion of an interfering element on a measurement ion of an analysis element measured by a mass spectrometer using a plasma ion source by accounting for a mass-bias effect of the mass spectrometer, measurement values of ionic strength of divalent ions of two isotopes having different, odd mass numbers among isotopes of the interfering element are used. In measuring to obtain a measurement value where a correction method of the present…
Superficially porous particles with precisely controlled particle density, and methods of preparation and use thereof
Granted: December 17, 2019
Patent Number:
10507408
The invention provides superficially porous metal oxide particles with precisely controlled particle density and to methods for their preparation and use, as well as to separation devices (e.g., high pressure liquid chromatography) having superficially porous particles.
One-pot multiplex gene synthesis
Granted: November 26, 2019
Patent Number:
10487323
The present invention provides methods for generating a library of synthetic polynucleotides. The present invention also provides methods for generating proteins encoded by the library of synthetic polynucleotides. In addition, provided herein are methods for determining the soluble expression of said proteins. This invention is based, in part, on the discovery of a method for selecting optimal oligonucleotides in combination with performing a phosphorylation reaction, ligation reaction…
