Target enrichment and labeling for multi-kilobase DNA
Granted: June 4, 2019
Patent Number:
10308979
This disclosure provides a method comprising: a) clamping the top and bottom strands of a double stranded DNA molecule to produce a duplex in which the top and bottom strands are linked; b) denaturing the duplex to produce a denatured product; and c) renaturing the denatured product in the presence of a labeled oligonucleotide that is complementary to a sequence of nucleotides in the double stranded DNA molecule, thereby producing a D-loop-containing product. Kits for performing the…
Column assembly for a gas chromatograph
Granted: May 28, 2019
Patent Number:
10302605
A column assembly for gas chromatography, which is coolable by a fluid, includes a capillary column, a heating element for heating at least a portion of the capillary column, and a tube having a lumen and in contact with at least one of the capillary column and the heating element. When energized, the heating element raises the temperature of the capillary column. To lower the temperature of the capillary column, a fluid flows through the tube, and heat is transferred from the capillary…
Fluidic valve with contactless force transmission for pressing together stator and rotor
Granted: May 28, 2019
Patent Number:
10302213
A fluidic valve for switching between different fluid coupling states includes a stator having at least one fluidic stator interface, a rotor having at least one fluidic rotor interface, wherein the rotor is rotatable relative to the stator to thereby switch the fluidic valve between a plurality of different fluid coupling states between the at least one fluidic stator interface and the at least one fluidic rotor interface, and a force transmission mechanism configured for pressing the…
Sealing configuration with metal-coated structure
Granted: May 28, 2019
Patent Number:
10300403
A sealing configuration is configured for sealingly coupling a first fluid flow path of a first device to a second fluid flow path of a second device. The sealing configuration comprises a contact surface comprising a structure configured for increasing a surface pressure when coupling the first device to the second device. The contact surface comprises a metal coating configured for increasing sealing.
Angular synchronization of stationary and orbiting plate scroll blades in a scroll pump using a metallic bellows
Granted: May 21, 2019
Patent Number:
10294939
Parts of a pump head of a scroll pump facilitate an assembly process in which the stationary and orbiting scroll blades of the pump are angularly aligned or synchronized with one another. A metallic bellows of the pump head provides a primary means of synchronizing the stationary and orbiting scroll blades. The assembly process may be carried out using a fixture configured to be mountable to an assemblage including the bellows and the orbiting plate scroll. The fixture has a reference…
Tandem collision/reaction cell for inductively coupled plasma-mass spectrometry (ICP-MS)
Granted: May 14, 2019
Patent Number:
10290482
A tandem collision/reaction cell for an inductively coupled plasma-mass spectrometry (ICP-MS) system includes a first ion guide, a second ion guide, and an intermediate electrode in the vicinity of an exit end of the first ion guide. A DC potential barrier is applied to the intermediate electrode. The cell may provide two or more stages of an ion-molecule collision process.
Plurality of transposase adapters for DNA manipulations
Granted: May 14, 2019
Patent Number:
10287622
The present invention relates to transposase adapters and uses thereof, including uses in preparing DNA molecules, in vitro amplification, sequencing of nucleic acids, and screening of DNA libraries for sequences of interest as well as nucleic acid delivery.
Uninterrupted fluid flow while modulating fluid
Granted: May 7, 2019
Patent Number:
10281058
A fluid processing device (10) for processing fluid, wherein the fluid processing device (10) comprises a first fluid drive unit (20) configured for driving a first fluid along a first flow path (85), a second fluid drive unit (20?) configured for driving a second fluid along a second flow path (86), and a fluidic switch (90) fluidically coupled to the first flow path (85) and to the second flow path (86) and configured for being switchable for transferring first fluid from the first…
System and method for generating high-voltage radio frequency signals using an electronically tuned resonator
Granted: May 7, 2019
Patent Number:
10284154
A system for generating a radio frequency (RF) signal at a drive frequency and a high voltage. The system includes a RF amplifier to amplify the voltage of a drive signal having a selected RF frequency. The amplified drive signal is used to drive a resonator to generate the RF signal such that the resonant frequency is the same or substantially the same as the drive frequency. A resonance tuning controller compares the drive frequency and the resonant frequency. If the resonant frequency…
Quantum cascade laser with serially configured gain sections
Granted: May 7, 2019
Patent Number:
10283936
An apparatus that includes a gain chip assembly, an external cavity, and a controller is disclosed. The gain chip assembly includes first and second gain chips that are coupled optically such that light travels serially between the first gain chip and the second gain chip, each gain chip is electrically biased. The electrical bias of the first gain chip is independent of the electrical bias of the second gain chip. The external cavity has a tunable wavelength selective filter that is…
Self cleaning gas-liquid separator for serial or parallel collection of liquid fractions
Granted: May 7, 2019
Patent Number:
10279294
An apparatus, process, and system are disclosed that effectively provide separation of a combined gas/liquid flow stream into its separated gas and liquid factions. The invention is primarily directed to the fields of preparative supercritical fluid chromatography (SFC) and supercritical fluid extraction (SFE), but will have other utilization and applicability where phases of dramatically different density, viscosity and volumetric flow require separation.
Wideband isolation directed by ion mobility separation for analyzing compounds
Granted: April 23, 2019
Patent Number:
10267765
In an ion mobility-mass spectrometry (IM-MS) system, an ion mass-isolated data set is acquired by operating a mass filter to apply a mass isolation window having an m/z width such that the mass isolation window moves through a sequence of window positions, each window position being defined by an IM drift time value and an m/z ratio value. The m/z width of the mass isolation window and the sequence of window positions are determined such that the mass isolation window captures ions in a…
System and method for facilitating manual sorting of objects
Granted: April 23, 2019
Patent Number:
10265733
An apparatus that facilitates the manual sorting of objects is disclosed. The apparatus includes a display surface having a surface that can be selectively illuminated and that is adapted for receiving the objects. An identification reader reads identification information stored on the objects. A controller causes an area on the display surface corresponding to one of the objects to be illuminated based on the identification information and indicates a location to which the illuminated…
ESD protected tubing for removing charge from lumen
Granted: April 9, 2019
Patent Number:
10254257
An electrostatic discharge protected device for conducting a fluid through at least one lumen includes a tubing having a hollow interior defining the at least one lumen. The tubing includes an electrically conductive section configured for conducting electric charge, generated within the at least one lumen when the fluid flows through the at least one lumen, away from the at least one lumen.
Transposase-random priming DNA sample preparation
Granted: March 26, 2019
Patent Number:
10240196
Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.
Method for unsupervised stain separation in pathological whole slide images
Granted: March 12, 2019
Patent Number:
10229495
A method of operating a data processing system to automatically process a color digital image of a specimen that has been stained with a first dye and a second different dye is disclosed. The method includes receiving a color image that includes a plurality of pixels, each pixel being characterized by a pixel vector having components determined by the intensity of light in each of a corresponding number of wavelength bands. A plurality of pixel vectors of the image are transformed to a…
Apparatus and method for facilitating manual sorting of slides
Granted: February 26, 2019
Patent Number:
10217011
The present invention includes an apparatus and method that facilitates the manual sorting of slides into slide folders. The apparatus includes a horizontal display surface and a controller configured to receive slides and folders into which the slides are to be placed (or sorted). The controller operates components of the apparatus to selectively illuminate the display surface to guide a user in placing folders at multiple folder locations and in sorting the slides into the folders. In…
Mixer bypass sample injection for liquid chromatography
Granted: February 19, 2019
Patent Number:
10209229
In a liquid chromatography system, a sample is injected into a column by flowing a solvent mixture from a mixer into the column along a solvent mixture flow path; and injecting a sample into the solvent mixture flow path downstream of the mixer. In another liquid chromatography system, a sample is injected into a column by loading an isolator fluid into a sample loop, loading a sample into the sample loop, and flowing the sample into the column as a plug in front of the isolator fluid.
Method for supplying gas for plasma based analytical instrument
Granted: February 19, 2019
Patent Number:
10211038
To achieve an effective gas filtering in a plasma spectrometric apparatus using a gas of a comparatively high consumption flow rate, and to improve the analytical ability, there is provided a plasma spectrometric apparatus containing a sample introducer for producing and delivering an injector gas containing an analyte sample, a plasma generator for generating plasma into which the injector gas is introduced, and an analyzer disposed subsequent to the plasma generator for analyzing the…
Microfluidic glycan analysis
Granted: February 19, 2019
Patent Number:
10209259
Microfluidic devices and methods for analyzing glycan profiles of glycoproteins are provided. Some embodiments of the devices comprise a deglycosylation column for cleaving glycans, an optional cleaning column for removing proteins, a trapping column for enriching glycans, and a separation column for resolving glycans. The devices and methods significantly improve the speed and sensitivity of glycan analysis.
