Cell-substrate impedance monitoring of cancer cells
Granted: August 20, 2024
Patent Number:
12066428
Methods of assessing cytolysis of cancer cells are provided, which include: providing a cell-substrate impedance monitoring device operably connected to an impedance analyzer, adding target cells characterized as cancer cells to a well of the device adding effector cells to the well to form a test well, where the effector cells are immune cells obtained or derived from a same patient as the target cells; monitoring cell-substrate impedance of the test well before and after adding the…
Headspace eliminating microtiter plate lid and method of optically measuring well oxygen concentration through the lid
Granted: August 13, 2024
Patent Number:
12059683
An implement for eliminating headspace in the testing space(s) (T9 or MPWell) of a test tube (T) or microtiter plate (MP), and methods of using such implements to measure oxygen concentration in a test sample. The implement projects into a test chamber (T9 or MPWell) to displace a portion of a fluid sample within the test chamber (T9 or MPWell) and has longitudinally extending grooves (109 and 229) through which the displaced fluidic content can be discharged from the test chamber (T9 or…
Sample handling and dispatching individual sample packages for fluid processing
Granted: August 6, 2024
Patent Number:
12055526
A sampling unit for handling a sample fluid includes a sample container having a length and being configured for receiving and storing the sample fluid, and a sample segment dispatching unit configured for providing a plurality of individual sample packages of the fluidic sample, each contained in a respective volume segment along the length of the sample container, and for individually dispatching each of the plurality of individual sample packages for further processing in a fluid…
Instrument closure including sensor retainer
Granted: August 6, 2024
Patent Number:
D1037883
Deparaffinization of tissue utilizing electric field
Granted: July 30, 2024
Patent Number:
12050163
Paraffin-embedded tissue is prepared removing paraffin from the tissue. The paraffin is removed by generating an electric field effective to produce plasma and direct charged species of the plasma to the paraffin, thereby rendering the paraffin responsive to the electric field. The electric field may move the paraffin out from the tissue due to electrostatic force. Movement of the paraffin may be assisted by moving an electrode utilized to generate the electric field relative to the…
Systems and methods for electronically and optically monitoring biological samples
Granted: July 30, 2024
Patent Number:
12049615
A system for electronically and optically monitoring biological samples, the system including: a multi-well plate having a plurality of wells configured to receive a plurality of biological samples, each of the wells having a set of electrodes and a transparent window on a bottom surface of the well that is free of electrodes; an illumination module configured to illuminate the wells; a cradle configured to receive the multi-well plate, the cradle having an opening on the bottom that…
Sequencing an insert and an identifier without denaturation
Granted: July 16, 2024
Patent Number:
12037640
Methods and kits for next-generation sequencing are disclosed. In some embodiments, the present methods comprise sequencing an insert and sequencing an insert without an intervening denaturation step. Decreased sequencing signal from insert sequences, the use of unlabeled nucleotides to form double stranded insert constructs, and the use of synthesis blocking nucleotides are also discussed.
Mass spectrometry ION source
Granted: July 9, 2024
Patent Number:
12033843
Provided herein is an ion source containing a plurality of components, at least one of which is partially coated with a layer of silicon. The ion source reduces reactivity between the sample and the carrier gas, reduces or eliminates tailing in ion chromatograms, and/or improves mass spectral fidelity. Also provided are methods of using the ion source in a mass spectrometer or gas chromatograph-mass spectrometer.
Air gap magnetic coupling with counterbalanced force
Granted: June 18, 2024
Patent Number:
12015320
A magnetic coupling assembly includes a first balancing magnet positioned and surrounding drive magnets and, across a gap, a second balancing magnet and surrounding driven magnets. The drive magnets when rotated drive the driven magnets to rotate. The balancing magnets generate a repulsive force, which counterbalances an attractive force generated by the drive magnets and driven magnets. The assembly may be utilized in a pump for contactless coupling of a motor shaft to a pump shaft. The…
Fluid pump with integrated cowling and discharge muffler
Granted: June 11, 2024
Patent Number:
12006937
A discharge muffler for a fluid pump is formed by a cowling and a muffler insert configured to be removably inserted into a muffler receptacle of the cowling. The muffler insert includes a muffler inlet, a muffler outlet, and a plurality of muffler walls. The pump includes a pumping stage, a motor, and a drive shaft coupled between the pumping stage and the motor. When the muffler insert is inserted into the muffler receptacle, the muffler inlet communicates with the pumping stage, and…
Polymerase mutants and use with 3?-OH unblocked reversible terminators
Granted: June 11, 2024
Patent Number:
12006519
Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3?-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.
Two-dimensional fluid separation with push-pull modulation
Granted: June 11, 2024
Patent Number:
12005376
A sample separation apparatus includes a first-dimension separation unit for separating the fluidic sample, having a first-dimension outlet for outputting the fluidic sample or fractions thereof, and a second-dimension separation unit for further separating the fluidic sample or fractions thereof. The second-dimension separation unit has a second-dimension inlet fluidically coupled to the first-dimension outlet. A modulation unit, coupled between the first-dimension outlet and the…
Instrument monitoring system
Granted: June 4, 2024
Patent Number:
12003904
Systems and methods are disclosed for capturing and interpreting data streams between an instrument and a controlling device. A processor is configured to receive a data stream sent by the instrument to the controlling device and identify data frames in the stream. The processor is configured to search for a bit pattern in the stream, identify bits corresponding to a message length of a first presumed data frame based on a location relative to the bit pattern, and identify a second…
Fitting assemblies for fluidic connections
Granted: June 4, 2024
Patent Number:
12000810
A fitting assembly (100) is provided. The fitting assembly (100) comprises a holder (102) that holds one or more fluidic seal assemblies. The fluidic seal assembly comprises a fitting (104), a ferrule (110) and a tube (112), such as a chromatography column, and optionally comprises a protrusion (118) and a compliant seal material (120). Fluidic connections for a gas chromatography instrument are also provided.
Fluid supply devices and fluid member for forming a mobile phase for a sample separating device
Granted: June 4, 2024
Patent Number:
12000808
A fluid supply device for providing a mobile phase for a sample separating device includes, a supply conduit for providing a fluid which forms at least a part of the mobile phase, a fluid valve which is fluidically coupled with the supply conduit and, depending on its switching state, enables or prevents a passing of the fluid from the supply conduit, an elastic buffer unit which is fluidically coupled upstream of the fluid valve with the supply conduit and which is configured for…
Instrument component organizer
Granted: June 4, 2024
Patent Number:
11999182
In some examples, an organizer may include a plurality of inserts. At least one insert of the plurality of inserts may include at least a front panel and an opposite back panel that form the at least one insert, and at least one instrument component disposed hi the at least one insert. The organizer may further include a binding mechanism that attaches the plurality of inserts.
Chromogenic peroxidase substrates
Granted: May 28, 2024
Patent Number:
11993810
Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as a rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.
Molecular vaccines for infectious disease
Granted: May 28, 2024
Patent Number:
11992518
The present invention relates to methods for construction of pharmamers i.e. vaccine components characterized by their multimerization domain and the attached biologically active molecules, and their use in preparation of vaccines that contains the pharmamers alone or in combination with other molecules. The individual molecules of the construct can be bound to each other or the multimerization domain(s) by covalent or non-covalent bonds, directly or via linkers. The invention further…
Use of polyamines as resolving agents for capillary electrophoresis of labeled glycans using gels
Granted: May 21, 2024
Patent Number:
11988633
The present disclosure provides methods of improving the resolution of labeled glycans in capillary electrophoresis techniques using a gel as a sieving matrix, by incorporating polyamines in the gel.
Deparaffinization of tissue by electric field generation and ionization
Granted: May 14, 2024
Patent Number:
11982606
Paraffin-embedded tissue, which may be disposed on a solid substrate, is prepared by a dry technique that removes paraffin from tissue without adding any liquid to the tissue, thereby rendering the tissue substantially free of paraffin. The dry technique may entail applying heat energy to the tissue effective to melt the paraffin and thereby render it flowable, and applying an electric field. The electric field is effective to impart electrical charge to the paraffin and to move the…
