Polymerase mutants and use with 3?-OH unblocked reversible terminators
Granted: June 11, 2024
Patent Number:
12006519
Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3?-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.
Instrument monitoring system
Granted: June 4, 2024
Patent Number:
12003904
Systems and methods are disclosed for capturing and interpreting data streams between an instrument and a controlling device. A processor is configured to receive a data stream sent by the instrument to the controlling device and identify data frames in the stream. The processor is configured to search for a bit pattern in the stream, identify bits corresponding to a message length of a first presumed data frame based on a location relative to the bit pattern, and identify a second…
Fitting assemblies for fluidic connections
Granted: June 4, 2024
Patent Number:
12000810
A fitting assembly (100) is provided. The fitting assembly (100) comprises a holder (102) that holds one or more fluidic seal assemblies. The fluidic seal assembly comprises a fitting (104), a ferrule (110) and a tube (112), such as a chromatography column, and optionally comprises a protrusion (118) and a compliant seal material (120). Fluidic connections for a gas chromatography instrument are also provided.
Fluid supply devices and fluid member for forming a mobile phase for a sample separating device
Granted: June 4, 2024
Patent Number:
12000808
A fluid supply device for providing a mobile phase for a sample separating device includes, a supply conduit for providing a fluid which forms at least a part of the mobile phase, a fluid valve which is fluidically coupled with the supply conduit and, depending on its switching state, enables or prevents a passing of the fluid from the supply conduit, an elastic buffer unit which is fluidically coupled upstream of the fluid valve with the supply conduit and which is configured for…
Instrument component organizer
Granted: June 4, 2024
Patent Number:
11999182
In some examples, an organizer may include a plurality of inserts. At least one insert of the plurality of inserts may include at least a front panel and an opposite back panel that form the at least one insert, and at least one instrument component disposed hi the at least one insert. The organizer may further include a binding mechanism that attaches the plurality of inserts.
Chromogenic peroxidase substrates
Granted: May 28, 2024
Patent Number:
11993810
Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as a rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.
Molecular vaccines for infectious disease
Granted: May 28, 2024
Patent Number:
11992518
The present invention relates to methods for construction of pharmamers i.e. vaccine components characterized by their multimerization domain and the attached biologically active molecules, and their use in preparation of vaccines that contains the pharmamers alone or in combination with other molecules. The individual molecules of the construct can be bound to each other or the multimerization domain(s) by covalent or non-covalent bonds, directly or via linkers. The invention further…
Use of polyamines as resolving agents for capillary electrophoresis of labeled glycans using gels
Granted: May 21, 2024
Patent Number:
11988633
The present disclosure provides methods of improving the resolution of labeled glycans in capillary electrophoresis techniques using a gel as a sieving matrix, by incorporating polyamines in the gel.
3D cell washer
Granted: May 14, 2024
Patent Number:
11982268
An autoclaving microplate washing system for cells and non-adhering three-dimensional (3D) cell cultures is provided. The autoclaving microplate washing system may include a dispensing pump; an aspirating peristaltic pump; and a processor configured to execute a cleaning control program, which when executed causes the autoclaving microplate washing system to independently control: the dispensing pump to control a first flow rate of a solution from a fluid reservoir through a plurality of…
Deparaffinization of tissue by electric field generation and ionization
Granted: May 14, 2024
Patent Number:
11982606
Paraffin-embedded tissue, which may be disposed on a solid substrate, is prepared by a dry technique that removes paraffin from tissue without adding any liquid to the tissue, thereby rendering the tissue substantially free of paraffin. The dry technique may entail applying heat energy to the tissue effective to melt the paraffin and thereby render it flowable, and applying an electric field. The electric field is effective to impart electrical charge to the paraffin and to move the…
Etched non-porous particles and method of producing thereof
Granted: April 23, 2024
Patent Number:
11964874
There is disclosed a method of producing etched non-porous particles. The method includes, in some examples, coating a non-porous particle with a hydrophilic polymer and treating the coated particle with acid or base. Also provided is etched non-porous particles capable of separating a variety of analytes, including biomolecules.
Multi-device removal and installation tool
Granted: April 9, 2024
Patent Number:
11955326
In some examples, a multi-device removal and installation tool may include a device removal tool including catch members engageable with an engagement face of a device to attach, upon engagement of the catch members with the engagement face, the device removal tool to the device. The catch members may engage the engagement face with a removal force that is sufficient to remove, upon engagement of the catch members with the engagement face, the device from an instrument. The multi-device…
Operation of an injector of a sample separation device
Granted: April 2, 2024
Patent Number:
11946913
An injector is configured for injecting a fluidic sample from an injector path into a mobile phase in a separation path between a fluid drive and a sample separation unit of a sample separation device. The injector includes a control unit configured for generating a first overpressure in a blocked first partial path of the injector path by a first pressure source, for generating a second overpressure in a blocked second partial path of the injector path by a second pressure source, for…
Booster plate
Granted: April 2, 2024
Patent Number:
D1021136
Method and system for determining integrated metabolic baseline and potential of living cells
Granted: March 26, 2024
Patent Number:
11940442
The current technology is related to methods for rapidly determining the metabolic baseline and potential of living cells. Embodiments relate to measuring the activity of each of the two major energy-generating pathways within the cell: mitochondrial respiration and glycolysis, first under baseline conditions, and again after applying a stress to the cells to demand increased energy supply. In some embodiments the stress may be applied by exposing the cells to a combination of two…
Preparation of tissue sections using fluorescence-based detection
Granted: March 26, 2024
Patent Number:
11940359
The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or…
Manufacture of a microfluidic component by additive manufacturing
Granted: March 19, 2024
Patent Number:
11931918
A microfluidic component for a sample separation apparatus includes a component body including ceramic and at least one microfluidic structure in the component body. The component body is manufactured by additive manufacturing, in particular by three-dimensional printing.
Dual feedback loop for precision high voltage power supply
Granted: March 19, 2024
Patent Number:
11936301
A high-voltage (HV) power supply outputs an output voltage based on a control signal produced by a dual analog/digital feedback loop. The control signal is determined at least in part by an error amplifier that receives a measurement signal, proportionally attenuated from the output voltage, and a digital-to-analog converter (DAC) output signal. An analog-to-digital converter (ADC) also receives the measurement signal and transmits it in digitized form to a digital processor. The digital…
Coupled-amplifier multi-frequency circuit topologies applicable to mass spectrometer radio-frequency drive systems
Granted: March 19, 2024
Patent Number:
11935735
A circuit and method for providing high-voltage radio-frequency (RF) energy to an instrument at multiple frequencies includes a plurality of inputs each configured to receive an RF voltage signal oscillating at a corresponding frequency, and a step-up circuit for generating magnified RF voltage signals based on the received RF voltage signals. The step-up circuit includes an LC network operable to isolate the RF voltage signals at the plurality inputs from one another while preserving a…
Multidimensional sample separation with pressure adjustment
Granted: March 19, 2024
Patent Number:
11933769
A multidimensional sample separation apparatus includes a first separation dimension for separating a fluidic sample, a second separation dimension for further separating the fluidic sample, a switching mechanism, and a control unit. The first separation dimension includes a first fluid drive unit and a first sample separation unit. The second separation dimension includes a second fluid drive unit for driving the separated fluidic sample, and second and third sample separation units…
