Restriction mediated quantitative polymerase chain reactions
Granted: March 12, 2024
Patent Number:
11926870
The present invention relates to the technical field of nucleic acid amplification using a Polymerase Chain Reaction (PCR). Specifically, the present invention relates to Polymerase Chain Reaction (PCR) primers and Polymerase Chain Reaction (PCR) nucleic acid amplification mixture and the use thereof in (quantitative) Polymerase Chain Reactions (PCR). Specifically, the present invention relates to Polymerase Chain Reaction (PCR) primers suitable for use in Restriction Mediated…
Mounting a sample separation unit by a swivel mechanism
Granted: March 5, 2024
Patent Number:
11921093
A mounting device for mounting a sample separation unit configured for separating, preferably chromatographically separating, compounds in a fluidic sample includes a first fluid connector configured for being mechanically and fluidically coupled with a first fluid interface of the sample separation unit, a second fluid connector configured for being mechanically and fluidically coupled with a second fluid interface of the sample separation unit, and a swivel mechanism configured for…
Label-free monitoring of excitation-contraction coupling and excitable cells using impedance based systems with millisecond time resolution
Granted: February 20, 2024
Patent Number:
11906508
A system for monitoring cells, which includes a device for monitoring cell-substrate impedance, the device having a plurality of wells on a nonconductive substrate, where each of the plurality of wells has an electrode array fabricated on the substrate for measurement of cell-substrate impedance; an impedance analyzer that measures cell-substrate impedance from the plurality of wells; electronic circuitry with multiple analogue-to-digital conversion channels, where the electronic…
Column depth measuring guide
Granted: February 6, 2024
Patent Number:
D1013542
Optical coupling and a modular optical device comprising the same
Granted: January 30, 2024
Patent Number:
11885676
The invention provides an optical coupling, comprising: a first coupling portion and a second coupling portion, the first and second coupling portions configured to mate in kinematic constraint via a first mounting interface and at least two further mounting interfaces, wherein: the first coupling portion comprises a first mounting element and the second coupling portion comprises a socket, and the first mounting element is receivable in the socket at the first mounting interface such…
Guide RNAs with chemical modification for prime editing
Granted: January 30, 2024
Patent Number:
11884915
Provided herein are compositions and methods for inducing CRISPR/Cas-based editing of a target nucleic acid (e.g., target DNA or target RNA) in vitro or in a cell, using modified prime editing guide RNAs (pegRNAs) that incorporate one or more chemically-modified nucleotides. The modified pegRNAs disclosed herein may be used to induce Cas-mediated incorporation of one or more nucleotide changes and/or targeted mutagenesis of a target nucleic acid. The nucleotide change can include, e.g.,…
Column depth measuring guide
Granted: January 30, 2024
Patent Number:
D1012743
Column depth measuring guide
Granted: January 30, 2024
Patent Number:
D1012742
Solutions of quaternary and tertiary ammonium cation detergents to denature proteins, and kits for making them
Granted: January 2, 2024
Patent Number:
11858961
Ammonium cation detergents comprising a quaternary or tertiary ammonium cation can be used as detergents to denature proteins and are particularly useful in denaturing glycoproteins or glycopeptides prior to enzymatic deglycosylation. Ammonium cation detergents with sulfate or sulfonate anions are particularly useful.
Compositions comprising chemically modified guide RNAs for CRISPR/Cas-mediated editing of HBB
Granted: December 26, 2023
Patent Number:
11851652
Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in…
Method and system for implementing augmented reality (AR)-based assistance within work environment
Granted: December 19, 2023
Patent Number:
11847751
Novel tools and techniques are provided for implementing augmented reality (AR)-based assistance within a work environment. In various embodiments, a computing system might receive, from a camera having a field of view of a work environment, first images of at least part of the work environment, the first images overlapping with a field of view of a user wearing an AR headset; might analyze the received first images to identify objects; might query a database(s) to determine a task…
Sample injection with fluidic connection between fluid drive unit and sample accommodation volume
Granted: December 5, 2023
Patent Number:
11835495
An injector, for injecting a fluidic sample into a flow path between a fluid drive and a sample separation unit, includes a sample accommodation volume, a sample drive, and a fluidic valve switchable to selectively couple the volume with the flow path or decouple the volume from the flow path. In an injection switching state, the fluid drive, the separation unit and the sample drive are coupled by the valve so that fluid driven by the sample drive and flowing from the volume to the…
Hybridization compositions and methods
Granted: December 5, 2023
Patent Number:
11834703
The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in hybridization. Compositions for use in the invention include an aqueous composition comprising at least one nucleic acid sequence and at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.
Stable label isotope tracing for untargeted data
Granted: November 14, 2023
Patent Number:
11817185
A method for analyzing samples utilizing stable label isotope tracing includes receiving mass spectrometry (MS) data generated by an MS system performing untargeted data acquisition on a plurality of samples, performing untargeted feature extraction on the unlabeled compound data to generate a data set of first extracted features, generating a plurality of empirical molecular formulas respectively corresponding to the first extracted features, performing targeted isotopologue extraction…
Use of tri- and tetra-hydroxyl quaternary ammonium compounds as resolving agents for electrophoretic separations
Granted: November 14, 2023
Patent Number:
11815488
The present disclosure provides methods of improving the resolution of analytes by in electrophoretic separations using a gel by incorporating into the gel an effective amount of one or more tri- or tetra-hydroxyl quaternary ammonium compounds, or a mixture of such compounds.
Purging bottle
Granted: November 14, 2023
Patent Number:
D1004796
Column depth measuring guide
Granted: November 14, 2023
Patent Number:
D1004454
Column depth measuring guide
Granted: November 14, 2023
Patent Number:
D1004453
Column depth measuring guide
Granted: November 14, 2023
Patent Number:
D1004452
Multi-dimensional liquid chromatography with second dimension having a variable flow rate
Granted: November 7, 2023
Patent Number:
11808742
A multi-dimensional liquid chromatography system includes first and second liquid chromatography systems. The first system is configured for providing a first chromatographic separation of a sample fluid comprised in a first mobile phase and to provide a first effluent including at least a portion of the separated sample fluid. The second system is configured for providing a second chromatographic separation of at least a portion of the first effluent comprised in a second mobile phase.…
