Molecular fabrication
Granted: January 10, 2023
Patent Number:
11549106
Provided herein is a method for fabricating transformable or transfectable molecules that includes an assembly reaction containing a variety of pre-made cassettes possessing ends that hybridize to one another, transforming or transfecting said molecules into a desired host cell and then selecting a transformed/transfected host cell containing plasmid molecules composed of said the cassettes. A kit for performing the method is also provided.
Methods to improve reduction or labeling of carbohydrates
Granted: January 3, 2023
Patent Number:
11543414
The present invention provides methods, devices, and kits to improve procedures for reducing carbohydrates, such as glycans released from glycoconjugates, or for labeling carbohydrates by reductive amination.
Use of transposase and Y adapters to fragment and tag DNA
Granted: December 27, 2022
Patent Number:
11535889
Described herein is an adapter comprising a population of first oligonucleotides, a second oligonucleotide and a third oligonucleotide, wherein the first oligonucleotides, the second oligonucleotide and the third oligonucleotide are hybridized together to produce a complex that comprises: (i) a first end comprising a transposase recognition sequence, (ii) a central single-stranded region of variable sequence and (iii) a second end comprising sequences that are non-complementary. A…
Chemically modified guide RNAS for CRISPR/Cas-mediated gene regulation
Granted: December 27, 2022
Patent Number:
11535846
Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in…
Deparaffinization of tissue utilizing electric field
Granted: December 13, 2022
Patent Number:
11525759
Paraffin-embedded tissue is prepared removing paraffin from the tissue. The paraffin is removed by generating an electric field effective to produce plasma and direct charged species of the plasma to the paraffin, thereby rendering the paraffin responsive to the electric field. The electric field may move the paraffin out from the tissue due to electrostatic force. Movement of the paraffin may be assisted by moving an electrode utilized to generate the electric field relative to the…
Sample injector with conduit tip penetrating into needle opening
Granted: December 6, 2022
Patent Number:
11519885
A sample injector for a chromatography system is configured for injecting a sample fluid into a mobile phase, and includes a needle and a conduit. The needle is configured for aspirating the sample fluid and includes a needle tip, a needle channel through the needle for guiding the aspirated sample fluid, and a needle opening at the needle tip into which the needle channel opens. The conduit is configured for fluidically coupling with the needle and includes a conduit tip, and a conduit…
Sample injector with metering device balancing pressure differences in an intermediate valve state
Granted: December 6, 2022
Patent Number:
11519884
A sample injector for use in a fluid separation system for separating compounds of a fluidic sample in a mobile phase, the sample injector comprising a switchable valve, a sample loop in fluid communication with the valve and configured for receiving the fluidic sample, a metering device in fluid communication with the sample loop and configured for introducing a metered amount of the fluidic sample on the sample loop, and a control unit configured for controlling switching of the valve…
Ion guide with varying multipoles
Granted: November 29, 2022
Patent Number:
11515137
An ion guide includes electrodes elongated along an axis from an entrance end to an exit end and spaced around the axis to surround an interior. The electrodes have polygonal shapes with inside surfaces disposed at a radius from the axis and having an electrode width tangential to a circle inscribed by the electrodes. An aspect ratio of the electrode width to the radius varies along the axis. The electrodes are configured to generate a two-dimensional RF electrical field in the interior…
Synthetic silica as packing material in supported liquid extraction
Granted: November 29, 2022
Patent Number:
11511257
In embodiments, a packing material for supported liquid extraction has a sorbent media that includes synthetic silica particles. In embodiments, the synthetic silica particles can have physical properties relating to one or more of particle surface area, shape, size, or porosity. In one embodiment, synthetic silica particles have a surface area less than about 30 m2/g. In another embodiment, the synthetic silica particles have an approximately uniform particle shape. In further examples,…
Mass spectrometry compatible salt formation for ionic liquid sample preparation
Granted: November 22, 2022
Patent Number:
11506581
Reagents and methods for obtaining a metabolite solution comprising a mass spectrometry compatible volatile salt or volatile compound.
Sequencing method for genomic rearrangement detection
Granted: November 22, 2022
Patent Number:
11505826
The present disclosure is directed to a single-end sequencing method for improved detection of genomic rearrangements such as deletions, insertions, inversions, and translocations that are present in a polynucleotide. A first priming event allows for sequencing of a target sequence, and a second priming event on an adapter allows for identification of the sequences amplified and tagged by selective amplification. The combination of priming events in the same direction facilitates read…
Method and system for implementing augmented reality (AR)-based assistance within work environment
Granted: November 8, 2022
Patent Number:
11494988
Novel tools and techniques are provided for implementing augmented reality (AR)-based assistance within a work environment. In various embodiments, a computing system might receive, from a camera having a field of view of a work environment, first images of at least part of the work environment, the first images overlapping with a field of view of a user wearing an AR headset; might analyze the received first images to identify objects; might query a database(s) to determine a task…
Gas-liquid separator for collecting chromatographic fractions
Granted: November 8, 2022
Patent Number:
11491419
A gas-liquid separator includes a fluid inlet, a shell including an inside surface enclosing an interior space, an outlet structure with fingers converging toward a longitudinal axis, and a dripper including a dripper tip. The fingers terminate at fingertips located proximate to an outside surface of the dripper. Gas exit ports are defined between adjacent fingers, and by the dripper. The gas-liquid separator defines a liquid flow path from the fluid inlet, along the inside surface,…
Vacuum system with a multi-stage and multi-inlet vacuum pump with a directional element separating pump stages
Granted: October 25, 2022
Patent Number:
11480181
The invention relates to a vacuum system, comprising a vacuum pump, preferably turbomolecular pump, and at least one vacuum chamber, wherein the vacuum pump comprises: at least a first and a second inlet and a common outlet; at least a first and a second pumping stage, each pumping stage comprising at least one rotor element being arranged on a common rotor shaft, wherein the first inlet is connected to an upstream end of the first pumping stage and the second inlet is connected to an…
Chromogenic peroxidase substrates
Granted: October 11, 2022
Patent Number:
11466314
Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as a rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.
Inductively coupled plasma mass spectrometry (ICP-MS) with ion trapping
Granted: September 13, 2022
Patent Number:
11443933
An inductively coupled plasma-mass spectrometry (ICP-MS) system includes an ion trap, in which ions are trapped and subsequent ejected by mass-selective ejection (MSE). The system may have a linear quadrupole configuration, in which the ion trap is a linear ion trap (LIT) that is preceded by a pre-LIT linear quadrupole device and/or a post-LIT quadrupole device. The pre-LIT and/or post-LIT quadrupole device may be configured or operated as an RF-only ion guide or as a mass filter or mass…
Highly automated capillary electrophoresis system
Granted: September 13, 2022
Patent Number:
11442038
The invention is an improved multiplex capillary electrophoresis instrument or module with at least four and preferably six user-accessible vertically stacked drawers. An x-z stage moves samples from the user accessible drawers to the capillary array for analysis. An additional mechanical stage moves the array from side-to-side. The x-z stage, coupled with the additional array stage allows the system to sample all wells of a 384 well plate with a 96-capillary array. A computer program…
Transposase-random priming DNA sample preparation
Granted: September 13, 2022
Patent Number:
RE49207
Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.
Unions and couplers
Granted: September 6, 2022
Patent Number:
11435328
One aspect of the invention provides a gas chromatography union including: a first port; a second port in fluid communication with the first port; and a first pair of wings defining a first recess. Another aspect of the invention provides a union including: a first port; a second port in fluid communication with the first port; and at least a first protrusion defining a first recess. Another aspect of the invention provides a coupler including: a first port; a second port in fluid…
Chemically modified guide RNAs for CRISPR/CAS-mediated gene regulation
Granted: April 19, 2022
Patent Number:
11306309
Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in…
