Purging bottle
Granted: April 11, 2023
Patent Number:
D983406
Real time electronic cell sensing systems and applications for cell-based assays
Granted: March 14, 2023
Patent Number:
11604197
Methods for identifying compounds that modulate cellular responses stimulated by IgE, which include providing an impedance-based system that monitors cell-substrate impedance of cells on a substrate; introducing cells to the substrate of the system; adding at least one test compound and IgE to the cells, wherein the at least one test compound is suspected of modulating cell responses stimulated by the IgE; adding an antigen to the cells; monitoring the cell-substrate impedance of cells…
Scoring methods for anti-PD therapy eligibility and compositions for performing same
Granted: February 21, 2023
Patent Number:
11585817
Aspects of the present disclosure provide methods for determining the eligibility of a subject having a malignancy for treatment with an anti-PD therapeutic agent based on a Combined Positive Score (CPS) for a tumor tissue sample from the subject. Compositions and kits or performing the disclosed methods are also provided.
Phosphorous protecting groups and methods of preparation and use thereof
Granted: February 21, 2023
Patent Number:
11584773
Aspects of the present disclosure include compositions that make use of phosphorus and/or nucleobase protecting groups which find use in the synthesis of long polynucleotides. Phosphorus protecting groups are provided that help increase the stepwise coupling yield and/or phosphorous protecting groups that can be removed during the oxidation step. Amidine nucleobase protecting groups are provided that find use in the subject compositions and methods which provides for e.g., increased…
Polychromator systems and methods
Granted: February 14, 2023
Patent Number:
11579459
A polychromator system comprising: an optical element defining an aperture; a collimation mirror for receiving light via the aperture and reflecting substantially collimated light; at least a first dispersive optical component and a second dispersive optical component, each configured to disperse the substantially collimated light received from the collimation mirror by different amounts for different wavelengths and to provide cross-dispersed light having different wavelengths of light…
Modified multiplex and multistep amplification reactions and reagents therefor
Granted: February 14, 2023
Patent Number:
11578357
The invention relates to reagents and methods for improving the efficiency of multiplex nucleic acid amplification, in particular where overlapping amplicons are to be generated. The invention also relates to reagents and methods for improving the efficiency of multistep nucleic acid amplification, in particular the performance of two separate amplification reactions designed to occur in sequence in the same reaction mixture or vessel. The invention further relates to reagents and…
Touchless package
Granted: February 7, 2023
Patent Number:
D977341
Injector with fluid supply and mobile phase discharge
Granted: January 31, 2023
Patent Number:
11567040
Injector (40) for injecting a fluidic sample into a mobile phase in a sample separation apparatus (10), the injector (40) comprising a main flow path (100) between a fluid drive (20) and a sample separation device (30), wherein the fluid drive (20) is adapted to drive the mobile phase and the sample separation device (30) is adapted to separate the fluidic sample that is injected into the mobile phase, a discharge device (104) for discharging an, in particular predetermined, amount of…
Precision high voltage power supply with dual feedback loop
Granted: January 17, 2023
Patent Number:
11557974
A high-voltage (HV) power supply outputs an output voltage based on a control signal produced by a dual analog/digital feedback loop. The control signal is determined at least in part by an error amplifier that receives a measurement signal, proportionally attenuated from the output voltage, and a digital-to-analog converter (DAC) output signal. An analog-to-digital converter (ADC) also receives the measurement signal and transmits it in digitized form to a digital processor. The digital…
Molecular fabrication
Granted: January 10, 2023
Patent Number:
11549106
Provided herein is a method for fabricating transformable or transfectable molecules that includes an assembly reaction containing a variety of pre-made cassettes possessing ends that hybridize to one another, transforming or transfecting said molecules into a desired host cell and then selecting a transformed/transfected host cell containing plasmid molecules composed of said the cassettes. A kit for performing the method is also provided.
Orthoester compositions for affinity purification of oligonucleotides
Granted: January 10, 2023
Patent Number:
11548876
Compounds and methods for purifying oligonucleotides such as RNA and DNA. A target oligonucleotide is reacted with an orthoester linker comprising an affinity tag to form an orthoester oligonucleotide-orthoester linker conjugate which is subjected to a purification technique to separate the target oligonucleotide from impurities such as truncated oligonucleotides. The orthoester linker can be then removed under mild conditions to generate the target oligonucleotide in high purity.
Methods to improve reduction or labeling of carbohydrates
Granted: January 3, 2023
Patent Number:
11543414
The present invention provides methods, devices, and kits to improve procedures for reducing carbohydrates, such as glycans released from glycoconjugates, or for labeling carbohydrates by reductive amination.
Use of transposase and Y adapters to fragment and tag DNA
Granted: December 27, 2022
Patent Number:
11535889
Described herein is an adapter comprising a population of first oligonucleotides, a second oligonucleotide and a third oligonucleotide, wherein the first oligonucleotides, the second oligonucleotide and the third oligonucleotide are hybridized together to produce a complex that comprises: (i) a first end comprising a transposase recognition sequence, (ii) a central single-stranded region of variable sequence and (iii) a second end comprising sequences that are non-complementary. A…
Chemically modified guide RNAS for CRISPR/Cas-mediated gene regulation
Granted: December 27, 2022
Patent Number:
11535846
Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in…
Deparaffinization of tissue utilizing electric field
Granted: December 13, 2022
Patent Number:
11525759
Paraffin-embedded tissue is prepared removing paraffin from the tissue. The paraffin is removed by generating an electric field effective to produce plasma and direct charged species of the plasma to the paraffin, thereby rendering the paraffin responsive to the electric field. The electric field may move the paraffin out from the tissue due to electrostatic force. Movement of the paraffin may be assisted by moving an electrode utilized to generate the electric field relative to the…
Sample injector with conduit tip penetrating into needle opening
Granted: December 6, 2022
Patent Number:
11519885
A sample injector for a chromatography system is configured for injecting a sample fluid into a mobile phase, and includes a needle and a conduit. The needle is configured for aspirating the sample fluid and includes a needle tip, a needle channel through the needle for guiding the aspirated sample fluid, and a needle opening at the needle tip into which the needle channel opens. The conduit is configured for fluidically coupling with the needle and includes a conduit tip, and a conduit…
Sample injector with metering device balancing pressure differences in an intermediate valve state
Granted: December 6, 2022
Patent Number:
11519884
A sample injector for use in a fluid separation system for separating compounds of a fluidic sample in a mobile phase, the sample injector comprising a switchable valve, a sample loop in fluid communication with the valve and configured for receiving the fluidic sample, a metering device in fluid communication with the sample loop and configured for introducing a metered amount of the fluidic sample on the sample loop, and a control unit configured for controlling switching of the valve…
Ion guide with varying multipoles
Granted: November 29, 2022
Patent Number:
11515137
An ion guide includes electrodes elongated along an axis from an entrance end to an exit end and spaced around the axis to surround an interior. The electrodes have polygonal shapes with inside surfaces disposed at a radius from the axis and having an electrode width tangential to a circle inscribed by the electrodes. An aspect ratio of the electrode width to the radius varies along the axis. The electrodes are configured to generate a two-dimensional RF electrical field in the interior…
Synthetic silica as packing material in supported liquid extraction
Granted: November 29, 2022
Patent Number:
11511257
In embodiments, a packing material for supported liquid extraction has a sorbent media that includes synthetic silica particles. In embodiments, the synthetic silica particles can have physical properties relating to one or more of particle surface area, shape, size, or porosity. In one embodiment, synthetic silica particles have a surface area less than about 30 m2/g. In another embodiment, the synthetic silica particles have an approximately uniform particle shape. In further examples,…
Mass spectrometry compatible salt formation for ionic liquid sample preparation
Granted: November 22, 2022
Patent Number:
11506581
Reagents and methods for obtaining a metabolite solution comprising a mass spectrometry compatible volatile salt or volatile compound.
