Mass spectrometry compatible salt formation for ionic liquid sample preparation
Granted: November 22, 2022
Patent Number:
11506581
Reagents and methods for obtaining a metabolite solution comprising a mass spectrometry compatible volatile salt or volatile compound.
Method and system for implementing augmented reality (AR)-based assistance within work environment
Granted: November 8, 2022
Patent Number:
11494988
Novel tools and techniques are provided for implementing augmented reality (AR)-based assistance within a work environment. In various embodiments, a computing system might receive, from a camera having a field of view of a work environment, first images of at least part of the work environment, the first images overlapping with a field of view of a user wearing an AR headset; might analyze the received first images to identify objects; might query a database(s) to determine a task…
Gas-liquid separator for collecting chromatographic fractions
Granted: November 8, 2022
Patent Number:
11491419
A gas-liquid separator includes a fluid inlet, a shell including an inside surface enclosing an interior space, an outlet structure with fingers converging toward a longitudinal axis, and a dripper including a dripper tip. The fingers terminate at fingertips located proximate to an outside surface of the dripper. Gas exit ports are defined between adjacent fingers, and by the dripper. The gas-liquid separator defines a liquid flow path from the fluid inlet, along the inside surface,…
Vacuum system with a multi-stage and multi-inlet vacuum pump with a directional element separating pump stages
Granted: October 25, 2022
Patent Number:
11480181
The invention relates to a vacuum system, comprising a vacuum pump, preferably turbomolecular pump, and at least one vacuum chamber, wherein the vacuum pump comprises: at least a first and a second inlet and a common outlet; at least a first and a second pumping stage, each pumping stage comprising at least one rotor element being arranged on a common rotor shaft, wherein the first inlet is connected to an upstream end of the first pumping stage and the second inlet is connected to an…
Chromogenic peroxidase substrates
Granted: October 11, 2022
Patent Number:
11466314
Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as a rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.
Inductively coupled plasma mass spectrometry (ICP-MS) with ion trapping
Granted: September 13, 2022
Patent Number:
11443933
An inductively coupled plasma-mass spectrometry (ICP-MS) system includes an ion trap, in which ions are trapped and subsequent ejected by mass-selective ejection (MSE). The system may have a linear quadrupole configuration, in which the ion trap is a linear ion trap (LIT) that is preceded by a pre-LIT linear quadrupole device and/or a post-LIT quadrupole device. The pre-LIT and/or post-LIT quadrupole device may be configured or operated as an RF-only ion guide or as a mass filter or mass…
Highly automated capillary electrophoresis system
Granted: September 13, 2022
Patent Number:
11442038
The invention is an improved multiplex capillary electrophoresis instrument or module with at least four and preferably six user-accessible vertically stacked drawers. An x-z stage moves samples from the user accessible drawers to the capillary array for analysis. An additional mechanical stage moves the array from side-to-side. The x-z stage, coupled with the additional array stage allows the system to sample all wells of a 384 well plate with a 96-capillary array. A computer program…
Transposase-random priming DNA sample preparation
Granted: September 13, 2022
Patent Number:
RE49207
Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.
Unions and couplers
Granted: September 6, 2022
Patent Number:
11435328
One aspect of the invention provides a gas chromatography union including: a first port; a second port in fluid communication with the first port; and a first pair of wings defining a first recess. Another aspect of the invention provides a union including: a first port; a second port in fluid communication with the first port; and at least a first protrusion defining a first recess. Another aspect of the invention provides a coupler including: a first port; a second port in fluid…
Chemically modified guide RNAs for CRISPR/CAS-mediated gene regulation
Granted: April 19, 2022
Patent Number:
11306309
Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in…
Mid-infrared scanning system
Granted: April 12, 2022
Patent Number:
11300773
An apparatus and method for generating a mid-infrared region image of a specimen are disclosed. The apparatus includes a mid-infrared region laser that generates a first light beam, and a stage adapted to carry a specimen to be scanned. An optical assembly focuses the first light beam to a point on the specimen. A first light detector measures a first intensity of light leaving the point on the specimen. A stage actuator assembly causes the specimen to move relative to the point in two…
Orthoester compositions for affinity purification of oligonucleotides
Granted: April 12, 2022
Patent Number:
11299483
Compounds and methods for purifying oligonucleotides such as RNA and DNA. A target oligonucleotide is reacted with an orthoester linker comprising an affinity tag to form an orthoester oligonucleotide-orthoester linker conjugate which is subjected to a purification technique to separate the target oligonucleotide from impurities such as truncated oligonucleotides. The orthoester linker can be then removed under mild conditions to generate the target oligonucleotide in high purity.
System and method for detecting multiple-excitation-induced light in a flow channel
Granted: March 29, 2022
Patent Number:
11287379
A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency…
Sample injection with fluidic connection between fluid drive unit and sample accommodation volume
Granted: March 15, 2022
Patent Number:
11275062
An injector, for injecting a fluidic sample into a flow path between a fluid drive and a sample separation unit, includes a sample accommodation volume, a sample drive, and a fluidic valve switchable to selectively couple the volume with the flow path or decouple the volume from the flow path. In an injection switching state, the fluid drive, the separation unit and the sample drive are coupled by the valve so that fluid driven by the sample drive and flowing from the volume to the…
Microfluidic check valve and related devices and systems
Granted: February 22, 2022
Patent Number:
11255465
A microfluidic check valve includes an inlet bore, an internal chamber, an outlet bore, and a disk freely movable in the chamber between an open position and a closed position. At the open position, the disk permits fluid to flow from the inlet bore, through the chamber, and to the outlet bore. At the closed position, the disk prevents fluid from flowing in the reverse direction from the chamber into the inlet bore. The check valve may be positioned in-line with a fluid conduit, and/or…
Microfluidic dielectrophoretic droplet extraction
Granted: February 22, 2022
Patent Number:
11253859
A microfluidic apparatus for separating a droplet of an emulsion in a microfluidic environment is described. The microfluidic apparatus includes a flow cell comprising a first microfluidic channel configured for flowing a first fluid through the flow cell and a second microfluidic channel configured for flowing a stream of a second fluid through the flow cell. The microfluidic apparatus further comprises a first electrode positioned at the first microfluidic channel and a second…
Method for finding species peaks in mass spectrometry
Granted: February 8, 2022
Patent Number:
11244818
A method for operating a data processing system to find peaks in a mass spectrum that includes an ordered set of measurements of the abundances of species as a function of the mass/charge ratio of the species is disclosed. The method includes selecting a candidate blob that has a plurality of blob peaks from the mass spectrum. The data processing system selects a candidate blob peak for characterization. The candidate blob peak is approximated by a first species peak using a species peak…
Preparation of tissue sections using fluorescence-based detection
Granted: February 8, 2022
Patent Number:
11243148
The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or…
Inductively coupled plasma mass spectrometer with mass correction
Granted: February 1, 2022
Patent Number:
11239068
Systems and methods for controlling mass filtering of polyatomic ions in an ion beam passing through an inductively coupled plasma mass spectrometer (ICP-MS). Polyatomic ion mass data representative of the exact mass of a polyatomic ion having a target isotope is determined. A control signal is generated based on the determined polyatomic ion mass data and output to an ICP-MS to filter based on mass the polyatomic ions in the ion beam traveling through the ICP-MS to an ion detector.
Dual material approach for high pressure bioinert flow path components
Granted: January 18, 2022
Patent Number:
11226316
A method of manufacturing a component having a flow path, wherein the method includes forming a high pressure resistant casing with a cavity therein, inserting a body of bioinert material into the cavity to thereby form a composite block, and further processing the composite block for at least partially forming the flow path defined by the component.
