DNA comprising regulatory regions from gene y of penicillium chrysogenum
Granted: May 5, 1998
Patent Number:
5747285
A gene expression system and a protein secretion system for eukaryotic, especially fungal, microorganisms has been provided for.
Longterm antimicrobial activity obtained by sustained release of hydrogen peroxide
Granted: May 5, 1998
Patent Number:
5747078
Methods for long-term preservation of food products using a lactoperoxidase system. Long-term preservation is achieved by controlled generation of hydrogen peroxide as a substrate for the lactoperoxidase system. Controlled production of hydrogen peroxide is achieved by generating the hydrogen peroxide enzymatically form a system wherein the substrate for the enzyme generating the hydrogen peroxide is immobilized or is generated in situ from a precursor.
Diamine salts for purification of clavulanic acid
Granted: April 21, 1998
Patent Number:
5741903
A process for the purification of clavulanic acid comprising reacting in an organic solvent impure clavulanic acid with a diamine of the formula ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4 are individually selected from the group consisting of alkyl of 1 to 8 carbon atoms, cycloalkyl of 3 to 8 carbon atoms and cycloalkyl alkyl of 3 to 8 cycloalkyl carbon atoms and alkyl of 1 to 8 carbon atoms, all optionally substituted with 1 to 3 members of the group consisting of halogen,…
Stable gene amplification in chromosomal DNA of prokaryotic microorganisms
Granted: March 31, 1998
Patent Number:
5733723
Transformed prokaryotic hosts are provided comprising two or more copies of a DNA sequence stably maintained in their chromosome, said DNA sequence comprising a gene encoding a polypeptide of interest wherein said copies are separated by endogenous chromosomal DNA sequences. Methods are also provided for producing said transformed host strains. The transformed host strains are capable of increased production of the polypeptide of interest compared to host strains which already produce…
Process for the production of 7-ADCA via expandase activity on penicillin G
Granted: March 24, 1998
Patent Number:
5731165
An overall process for the preparation and recovery of 7-aminodesacetoxycephalosporanic acid (7-ADCA) via enzymatic ring expansion activity on penicillin G, using a Penicillium chrysogenum transformant strain expressing expandase.
Vectors for use in filamentous fungi
Granted: March 17, 1998
Patent Number:
5728547
Novel vectors are disclosed for use in filamentous fungi such as Aspergillus sp. in particular, whereby protein coding regions may be inserted therein to achieve expression or expression followed by secretion of the coded protein from the host. Signal peptide sequences and promoter sequences valuable for this purpose are disclosed as are expression vectors containing coding regions native or foreign to the fungal host. In accordance with the invention, a filamentous fungus such as…
Process for the efficient production of 7-ADCA via 2-(carboxyethylthio)acetyl-7-ADCA and 3-(carboxymethylthio)propionyl-7-ADCA
Granted: March 10, 1998
Patent Number:
5726032
An overall efficient process for the preparation and recovery of 7-aminodesacetoxycephalosporanic acid (7-ADCA) via 2-(carboxyethylthio)acetyl- and 3-(carboxymethylthio)propionyl-7-ADCA, using a Penicillium chrysogenum transformant strain expressing expandase in conjunction with acyltransferase, is provided.
Gram-negative alkaliphilic microorganisms
Granted: February 17, 1998
Patent Number:
5719047
Gram-negative bacteria, which are obligate alkaliphiles, have been isolated from samples of soil, water and sediment and a number of other sources obtained from in and around soda lakes. These bacteria have been analyzed according to the principles of numerical taxonomy with respect to each other, as well as to a variety of known bacteria. In addition, these bacteria are further circumscribed by an analysis of various chemotaxonomic characteristics. The bacteria produce various…
Diamine salts of clavulanic acid
Granted: February 17, 1998
Patent Number:
5719277
New diamine mono and diamine di salts of clavulanic acid, pharmaceutical compositions comprising the same, and a new process using these diamine salts in the preparation of clavulanic acid and salts and esters thereof have been provided.
Method for the preparation of feed pellets
Granted: February 10, 1998
Patent Number:
5716655
The present invention discloses a method for obtaining feed pellets. The method comprises the addition of active ingredients to feed pellets after the pellets have been extruded. The method further comprises the addition of a solution or suspension of the desired feed or ingredient in a water or oil phase to the pellets under reduced pressure and subsequently increasing the pressure. The method results in pellets with a high degree of loading and in which the active ingredients are…
Transformed industrial bacillus strains and methods for making and using them
Granted: February 10, 1998
Patent Number:
5716807
Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected. Efficient transfer of the vehicle containing…
Dry yeast compositions and processes for preparing the same
Granted: February 10, 1998
Patent Number:
5716654
Dry granular yeast compositions for use in the production of bakery products and beverages. In particular, a dry granular yeast composition having a moisture content of not more than about 8% (w/w) and containing about 0.1 to 8% (w/w) of a bread-improving agent, wherein the dry yeast is present in granular form and said bread-improving agent is a coating on the dry yeast granules in the form of a film or adhered particles.
Astaxanthin-producing yeast cells methods for their preparation and their use
Granted: January 27, 1998
Patent Number:
5712110
An isolated pure culture of a strain of Phaffia rhodozyma which produces astaxanthin in an amount of at least 600 .mu.g per g Phaffia rhodozyma dry matter, as determined by HPLC analysis.
Astaxanthin-producing yeast cells, methods for their preparation and their use
Granted: January 20, 1998
Patent Number:
5709856
An isolated pure culture of a strain of Phaffia rhodozyma which produces astaxanthin in an amount of at least 600 .mu.g per g Phaffia rhodozyma dry matter, as determined by HPLC analysis.
Production of heterologous proteins in filamentous fungi
Granted: January 20, 1998
Patent Number:
5710021
Expression of proteins in filamentous fungal hosts is achieved in the presence of glucose using promoter variants in which creA binding sites are functionally disrupted.
Modified signal sequences
Granted: January 6, 1998
Patent Number:
5705362
The present invention describes a method for reducing processing ambiguity of signal peptidases. Specifically, the C-terminus of a signal sequence is mutated in a such a manner that the secreted protein contains the correct N-terminal amino acid.Furthermore, the present invention provides an hIL-3 which is essentially homogenous at its N-terminus.
Penicillin G acylase, a gene encoding the same and a method for the production of this enzyme
Granted: December 9, 1997
Patent Number:
5695978
The invention provides a gene encoding penicillin G acylase, the enzyme encoded by said gene and a method for the production of said enzyme by incorporating said gene in a host and bringing the same to expression. The gene is preferably obtained from a strain of the microorganism Alcaligenes faecalis.
Ceramide 3 derivatives based on monounsaturated fatty acids
Granted: December 2, 1997
Patent Number:
5693677
The present invention discloses ceramide 3 derivatives based on monounsaturated fatty acids. A preferred compound is N-oleoylphytosphingosine (Ceramide IIIB). A method for preparing these compounds is also disclosed. The solubility of these compounds is higher than the solubility of the corresponding saturated ceramide 3 derivatives, whereas their stability is comparable to saturated ceramide 3 stabilities. In addition, a method is described for maintaining the water permeability…
Cloning and expression of acetyl xylan esterases from fungal origin
Granted: October 28, 1997
Patent Number:
5681732
Methods and DNA constructs are provided for the expression of a fungal acetyl xylan esterase gene in microbial hosts. A purified fungal acetyl xylan esterase is obtained which is suited for the use as an accessory enzyme in the degradation of acetylated xylans.
Astaxanthin-producing yeast cells, methods for their preparation and their use
Granted: October 21, 1997
Patent Number:
5679567
An isolated pure culture of a strain of Phaffia rhodozyma which produces astaxanthin in an mount of at least 600 .mu.g per g Phaffia rhodozyma dry matter, as determined by HPLC analysis.
