Production of heterologous proteins in filamentous fungi
Granted: October 7, 1997
Patent Number:
5674707
Expression of proteins in filamentous fungal hosts is achieved in the presence of glucose using promoter variants in which creA binding sites are functionally disrupted.
Oxido reductase enzyme system obtainable from P. chrysogenum, the set of genes encoding the same and the use of oxido reductase enzyme systems or genes encoding the same for increasing antibiotic production
Granted: July 29, 1997
Patent Number:
5652132
A new oxido reductase enzyme activity obtainable from P. chrysogenum, involved in the production of .beta.-lactams, the set of genes encoding said enzyme activity, and a method to enhance said production by using the above-mentioned oxido reductase system and other oxido reductase systems or genes encoding for the same have been disclosed.
Baking improver compositions
Granted: July 22, 1997
Patent Number:
5650188
A baking improver composition in a homogeneous solid shaped form which includes one or more baking improver agents and at least one binding agent which permits disintegration of the solid form when in use for mixing a dough. Such a composition form may weigh between 25 grams and 2 kilograms and is capable of manual division into smaller dosage units and obtained, for example, by compacting a powder composition based on flour and containing about 20 to 30% by weight of lecithin as a…
Strains of Phaffia rhodozyma containing high levels of astaxanthin and low levels of 3-hydroxy-3',4'-didehydro-.beta., .PSI.-caroten-4-one (HDCO)
Granted: July 15, 1997
Patent Number:
5648261
The present invention provides Phaffia rhodozyma strains having increased levels of astaxantin and a low percentage of 3-hydroxy-3',4'-didehydro-.beta.,.psi.-caroten-4-one (HDCO). Such strains are obtained by a combination of mutagenesis and selection. The invention further provides a method for obtaining such strains. The invention also provides astaxanthin obtained from a mutagenized strain of Phaffia rhodozyma characterized in that it contains a decreased relative amount of HDCO.
Intersexual heterozygous phycomyces
Granted: July 1, 1997
Patent Number:
5643719
The present invention discloses intersexual heterozygous Phycomyces strains. These strains show an improved .beta.-carotene productivity and an increased stability when compared with both the wild type and heterokaryotic Phycomyces strains. The present invention also discloses a method for obtaining Phycomyces transformants without using selection markers. Furthermore, a process is provided for manufacturing .beta.-carotene comprising the use of intersexual heterozygous Phycomyces…
Pectinesterase in the treatment of fruit and vegetables
Granted: June 17, 1997
Patent Number:
5639494
A method to prepare a food containing a gel of a low-methoxylated pectin of at least one fruit or vegetable is disclosed. The method comprises adding pectin esterase to the fruit or vegetable or to the pulp thereof in an amount effective to demethoxylate pectin, optionally adding calcium chloride, and maintaining the fruit or vegetable or pulp under conditions such that the pectin esterase converts high-methoxylated pectin of the fruit or vegetable or pulp to low-methoxylated pectin and…
Selective N-acylation of amino alcohols
Granted: May 20, 1997
Patent Number:
5631356
The present invention provides an efficient method for the production of N-acyl amino alcohols via the selective acylation of the free amines of amino alcohols by reacting with an organic acid or a salt thereof with an alkyl sulfonyl chloride or an alkyl phenyl sulfonyl chloride, in an organic solvent and in the presence of an organic base, to form the corresponding mixed anhydride, followed by reacting the mixed anhydride with an amino alcohol or a salt thereof to form the corresponding…
Recombinant bioprocess for the preparation of 7-amino cephalosporanic acid (7-ACA)
Granted: May 13, 1997
Patent Number:
5629171
Important intermediates for preparing cephalosporin antibiotics, 7-amino-cephalosporanic acid (7-ACA) and 7-aminodeacetylcephalosporanic acid (7-ADAC), are prepared by a novel bioprocess in which a transformed Penicillium chrysogenum strain is cultured in the presence of an adipate feedstock to produce adipoyl-6-APA (6-amino penicillanic acid); followed by the in situ expression of the following genes with which the P. chrysogenum has been transformed:1) an expandase gene, whose…
Transformed industrial bacillus strains and methods for making and using them
Granted: April 29, 1997
Patent Number:
5624829
Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected. Efficient transfer of the vehicle containing…
Cloning and expression of the exo-polygalacturonase gene from aspergillus
Granted: April 29, 1997
Patent Number:
5624834
The present invention discloses a DNA sequence encoding the exo-polygalacturonase gene form Aspergillus. Specifically the Aspergillus tubigensis exo-polygalacturonase gene is cloned and expressed. The invention relates to vectors comprising the exo-polygalacturonase coding sequence and to host cells transformed with such vectors. The invention further relates to the production of recombinant exo-polygalacturonase and the use of this protein.
Enzymatic synthesis of ceramides and hybrid ceramides
Granted: March 11, 1997
Patent Number:
5610040
The present invention provides an efficient method for the production of ceramides and hybrid ceramides via the selective N-acylation of glycosphingolipids by reacting an organic acid or ester thereof with a lysosphingolipid in the presence of a lipase in an organic medium.
Cloning and expression of xylanase B
Granted: March 11, 1997
Patent Number:
5610046
Method and expression constructs are provided for the cloning of genes from fungal origin which encode polypeptides having the activity of the Aspergillus tubigensis XYL B enzyme and their overexpression in a selected microbial host cell. The present invention provides for the high level production of XYL B which may be used in a variety of industrial applications.
Astaxanthin-producing yeast cells, methods for their preparation and their use
Granted: February 4, 1997
Patent Number:
5599711
An isolated pure culture of a strain of Phaffia rhodozyma which produces astaxanthin in an amount of at least 600 .mu.g per g Phaffia rhodozyma dry matter, as determined by HPLC analysis.
Fungicide composition to prevent the growth of mould on foodstuff and agricultural products
Granted: January 28, 1997
Patent Number:
5597598
An antifungal composition is disclosed which comprises an antifungal agent of the polyene type, an acidific antifungal compound and an additional organic acid or its alkali or earth alkali salt. The use of this composition to treat food and agricultural products is disclosed.
Expression of phytase in plants
Granted: January 14, 1997
Patent Number:
5593963
The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific,…
Process for the production of juices from fruits and vegetables
Granted: November 26, 1996
Patent Number:
5578335
The present invention discloses the application of a pectin esterase substantially free from depolymerase activity in the maceration of apple pulp. The use of purified pectin esterase leads to a low amount of pectin degradation products in the juice and to the conservation of protopectin in the apple pomace. Furthermore, the juice yield and the pressability are increased through the use of purified PE.
Process for obtaining aroma components and aromas from their precursors of a glycosidic nature, and aroma components and aromas thereby obtained
Granted: November 12, 1996
Patent Number:
5573926
A method is disclosed for obtaining aroma components and aromas from their glycosidic precursors containing a .beta.-apioside with the use of .beta.-apiosidase.
Gram-negative alkaliphilic microorganisms
Granted: November 5, 1996
Patent Number:
5571716
Gram-negative bacteria, which are obligate alkaliphiles, have been isolated from samples of soil, water and sediment and a number of other sources obtained from in and around soda lakes. These bacteria have been analyzed according to the principles of numerical taxonomy with respect to each other, as well as to a variety of known bacteria. In addition, these bacteria are further circumscribed by an analysis of various chemotaxonomic characteristics. The bacteria produce various…
Stable natamycin suspensions
Granted: September 3, 1996
Patent Number:
5552151
Concentrated suspensions of polyene fungicides such as natamycin are disclosed which exhibit good chemical, microbial and physical stability and which are thus particularly suitable as stock suspensions for the preparation of immersion liquids and coating emulsions for treatment of food, feed and agricultural products. Such suspensions have a pH in the range 3 to 4.8 and include a thickening agent.
Compositions and method for improving flour dough
Granted: August 20, 1996
Patent Number:
5547690
A method for improving the rheological properties of a flour dough through the use of an enzyme preparation which contains sulfhydryl oxidase and glucose oxidase.
