STRUCTURE-ACTIVITY RELATIONSHIPS
Granted: June 27, 2013
Application Number:
20130165341
The present disclosure relates to compositions and methods for screening a plurality of polypeptide variants.
BIOCATALYSTS AND METHODS FOR THE SYNTHESIS OF (1R,2R)-2-(3,4-DIMETHOXYPHENETHOXY)CYCLOHEXAMINE
Granted: June 27, 2013
Application Number:
20130164794
The disclosure provides transaminase polypeptides capable of converting the substrate, 2-(3,4-dimethoxyphenethoxy)cyclohexanone to the trans diastereomer product (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine in at least a 2:1 diastereomeric ratio relative to the cis diastereomer (1R,2S)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine. The disclosure also provides polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides in processes for preparing…
STRUCTURE-ACTIVITY RELATIONSHIPS
Granted: June 20, 2013
Application Number:
20130157900
The present disclosure relates to compositions and methods for screening a plurality of polypeptide variants.
COMBINED AUTOMATED PARALLEL SYNTHESIS OF POLYNUCLEOTIDE VARIANTS
Granted: June 6, 2013
Application Number:
20130143767
The present disclosure relates to methods for efficient synthesis, cloning, transformation and screening of large diverse libraries of polynucleotide variants comprising well-defined nucleotide differences relative to a reference polynucleotide.
PRODUCTION OF MONOTERPENES
Granted: June 6, 2013
Application Number:
20130143291
The present invention relates to methods for producing monoterpenes, particularly tricyclene, by culturing microbial organisms that express a terpene synthase and optionally a prenyl transferase.
VARIANT LOVD POLYPEPTIDES AND THEIR USES
Granted: May 23, 2013
Application Number:
20130130351
The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound
ENONE REDUCTASES
Granted: May 9, 2013
Application Number:
20130115663
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
BETA-GLUCOSIDASE VARIANT ENZYMES AND RELATED POLYNUCLEOTIDES
Granted: April 18, 2013
Application Number:
20130095530
The invention provides variants of the Azospirillum irakense CelA ?-glucosidase that have improve ?-glucosidase activity, particularly improved thermoactivity, compared to the wild type enzyme. The invention further provides related polynucleotides, vectors, host cell, and methods for making and using the variants.
BIOCATALYSTS AND METHODS FOR THE SYNTHESIS OF (S)-3-(1-AMINOETHYL)-PHENOL
Granted: April 11, 2013
Application Number:
20130089898
The present disclosure provides engineered transaminase polypeptides having improved properties as compared to naturally occurring transaminases including the ability of converting the substrate, 3?-hydroxyacetophenone to (S)-3-(1-aminoethyl)-phenol in enantiomeric excess and high percentage conversion. Also provided are polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases…
FUNGAL PROTEASES
Granted: April 4, 2013
Application Number:
20130084608
The present invention provides fungal proteases and improved fungal strains that are deficient in protease production.
KETOREDUCTASE POLYPEPTIDES FOR THE REDUCTION OF ACETOPHENONES
Granted: March 28, 2013
Application Number:
20130078692
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
GH61 GLYCOSIDE HYDROLASE PROTEIN VARIANTS AND COFACTORS THAT ENHANCE GH61 ACTIVITY
Granted: February 28, 2013
Application Number:
20130052713
The present invention provides various GH61 protein variants comprising various amino acid substitutions. The GH61 protein variants have an improved ability to synergize with cellulase enzymes, thereby increasing the yield of fermentable sugars obtained by saccharification of biomass. In some embodiments, sugars obtained from saccharification are fermented to produce numerous end-products, including but not limited to alcohol.
BIOCATALYSTS FOR EZETIMIBE SYNTHESIS
Granted: February 28, 2013
Application Number:
20130052699
The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
GH61 GLYCOSIDE HYDROLASE PROTEIN VARIANTS AND COFACTORS THAT ENHANCE GH61 ACTIVITY
Granted: February 28, 2013
Application Number:
20130052698
The present invention provides various GH61 protein variants comprising various amino acid substitutions. The GH61 protein variants have an improved ability to synergize with cellulase enzymes, thereby increasing the yield of fermentable sugars obtained by saccharification of biomass. In some embodiments, sugars obtained from saccharification are fermented to produce numerous end-products, including but not limited to alcohol.
CELLOBIOHYDROLASE VARIANTS
Granted: February 28, 2013
Application Number:
20130052693
The present invention relates to cellobiohydrolase variants having improved thermostability in comparison to wild-type CBH2a.
POLYNUCLEOTIDES ENCODING KETOREDUCTASES FOR PRODUCING STEREOISOMERICALLY PURE STATINS AND SYNTHETIC INTERMEDIATES THEREFOR
Granted: February 14, 2013
Application Number:
20130040364
The present disclosure provides ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, method of using the engineered ketoreductase enzymes to synthesize a variety of chirally pure compounds, and the chirally pure compounds prepared therewith.
PRODUCTION OF FATTY ALCOHOLS WITH FATTY ALCOHOL FORMING ACYL-COA REDUCTASES (FAR)
Granted: February 14, 2013
Application Number:
20130040352
The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF AZETIDINONE
Granted: February 7, 2013
Application Number:
20130034895
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Processes Using Amino Acid Dehydrogenases and Ketoreductase-Based Cofactor Regenerating System
Granted: January 31, 2013
Application Number:
20130029385
The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.
Synthesis of Prazole Compounds
Granted: January 17, 2013
Application Number:
20130017580
The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
