POLYNUCLEOTIDES ENCODING RECOMBINANT KETOREDUCTASE POLYPEPTIDES
Granted: December 20, 2012
Application Number:
20120322136
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered…
ENDOGLUCANASES
Granted: December 13, 2012
Application Number:
20120315676
The present invention relates to variant endoglucanases and particularly endoglucanases having improved properties over wild-type endoglucanase.
Substantially Stereomerically Pure Fused Bicyclic Proline Compounds and Processes for Preparing Boceprevir
Granted: November 15, 2012
Application Number:
20120289709
The present disclosure provides substantially enantiomerically pure heterobicyclic compounds of the following structural formulas, wherein A, M, M?, and R5 are as described herein, and to biocatalytic processes for their preparation, and to the enzymes used in those processes.
CELLOBIOHYDROLASE VARIANTS
Granted: November 1, 2012
Application Number:
20120276594
The present invention relates to cellobiohydrolase variants having improved thermostability and/or thermoactivity in comparison to wild-type Myceliophthora thermophila CBH2b.
KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF (R)-3-HYDROXYTHIOLANE
Granted: November 1, 2012
Application Number:
20120276599
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.
COMPOSITIONS AND METHODS FOR PRODUCTION OF FERMENTABLE SUGARS
Granted: October 25, 2012
Application Number:
20120270278
The present application provides genetically modified fungal organisms that produce enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to glucose, enzyme mixtures produced by the genetically modified fungal organisms, and processes for producing glucose from cellulose using such enzyme mixtures.
PENICILLIN-G ACYLASES
Granted: October 25, 2012
Application Number:
20120270282
The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
METHODS FOR GENERATING POLYNUCLEOTIDES HAVING DESIRED CHARACTERISTICS BY ITERATIVE SELECTION AND RECOMBINATION
Granted: October 4, 2012
Application Number:
20120252701
A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
METHODS OF POPULATING DATA STRUCTURES FOR USE IN EVOLUTIONARY SIMULATIONS
Granted: October 4, 2012
Application Number:
20120252684
In particular, this invention provides novel methods of populating data structures for use in evolutionary modeling. In particular, this invention provides methods of populating a data structure with a plurality of character strings. The methods involve encoding two or more biological molecules into character strings to provide a collection of two or more different initial character strings; selecting at least two substrings from the pool of character strings; concatenating the…
EVOLUTION OF WHOLE CELLS AND ORGANISMS BY RECURSIVE SEQUENCE RECOMBINATION
Granted: October 4, 2012
Application Number:
20120252681
The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
PENTOSE FERMENTATION BY A RECOMBINANT MICROORGANISM
Granted: October 4, 2012
Application Number:
20120252074
The present invention provides methods and compositions suitable for use in the conversion of xylose to xylitol and xylulose, including nucleic acid constructs, recombinant fungal host cells, and related materials.
Biocatalytic Processes for the Preparation of Stereomerically Pure Fused Bicyclic Proline Compounds
Granted: September 27, 2012
Application Number:
20120244581
The present disclosure provides substantially enantiomerically pure heterobicyclic compounds of the following structural formulas, wherein A, M, M?, and R5 are as described herein, and to biocatalytic processes for their preparation, and to the enzymes used in those processes.
LOV-D ACYLTRANSFERASE MEDIATED ACYLATION
Granted: September 27, 2012
Application Number:
20120244582
Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.
MULTI-CELLULASE ENZYME COMPOSITIONS FOR HYDROLYSIS OF CELLULOSIC BIOMASS
Granted: September 13, 2012
Application Number:
20120231510
The invention relates to a multi-cellulase enzyme composition for the enzymatic hydrolysis of cellulosic biomass said composition comprising a cellobiohydrolase (CBH) enzyme, an endoglucanase (EG) enzyme and a ?-glucosidase (BG) enzyme.
Recombinant Halohydrin Dehalogenase Polypeptides
Granted: August 30, 2012
Application Number:
20120220002
The present disclosure provides engineered halohydrin dehalogenase (HHDH) polypeptides having improved enzyme properties as compared to the wild-type HHDH enzyme HheC and other reference engineered HHDH polypeptides. Also provided are polynucleotides encoding the engineered HHDH enzymes, host cells capable of expressing the engineered HHDH enzymes, and methods of using the engineered HHDH enzymes to synthesize a variety of chiral compounds including chiral epoxides and chiral alcohols.
ENDOGLUCANASE VARIANTS
Granted: August 16, 2012
Application Number:
20120208235
The present invention relates to variant endoglucanases having improved thermoactivity, improved thermostability, and improved viscosity reduction activity over wild-type M. thermophila endoglucanase.
Halohydrin Dehalogenases and Related Polynucleotides
Granted: August 16, 2012
Application Number:
20120208259
The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.
RECOMBINANT C1 BETA-GLUCOSIDASE FOR PRODUCTION OF SUGARS FROM CELLULOSIC BIOMASS
Granted: July 26, 2012
Application Number:
20120190076
The invention relates to expression of a recombinant C1 ?-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting celiobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 ?-glucosidase, operably linked to heterologous promoter, under conditions in which ?-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said ?-glucosidase to glucose. Methods of the…
Transaminase Reactions
Granted: July 26, 2012
Application Number:
20120190085
The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.
KETOREDUCTASE-MEDIATED STEREOSELECTIVE ROUTE TO ALPHA CHLOROALCOHOLS
Granted: July 26, 2012
Application Number:
20120190086
The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ? chloroalcohols from ? chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides.
