Halohydrin Dehalogenases and Related Polynucleotides
Granted: August 16, 2012
Application Number:
20120208259
The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.
ENDOGLUCANASE VARIANTS
Granted: August 16, 2012
Application Number:
20120208235
The present invention relates to variant endoglucanases having improved thermoactivity, improved thermostability, and improved viscosity reduction activity over wild-type M. thermophila endoglucanase.
RECOMBINANT C1 BETA-GLUCOSIDASE FOR PRODUCTION OF SUGARS FROM CELLULOSIC BIOMASS
Granted: July 26, 2012
Application Number:
20120190076
The invention relates to expression of a recombinant C1 ?-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting celiobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 ?-glucosidase, operably linked to heterologous promoter, under conditions in which ?-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said ?-glucosidase to glucose. Methods of the…
Transaminase Reactions
Granted: July 26, 2012
Application Number:
20120190085
The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.
KETOREDUCTASE-MEDIATED STEREOSELECTIVE ROUTE TO ALPHA CHLOROALCOHOLS
Granted: July 26, 2012
Application Number:
20120190086
The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ? chloroalcohols from ? chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides.
KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF (S,E)-METHYL 2-(3-(3-(2-(7-CHLOROQUINOLIN-2-YL)VINYL)PHENYL)-3-HYDROXYPROPYL)BENZOATE
Granted: July 19, 2012
Application Number:
20120184000
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
ENDOGLUCANASES
Granted: July 12, 2012
Application Number:
20120178132
The present invention relates to variant endoglucanases and particularly endoglucanases having improved properties over wild-type endoglucanase.
Ketoreductases and Uses Thereof
Granted: July 12, 2012
Application Number:
20120178142
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
RECOMBINANT BETA-GLUCOSIDASE VARIANTS FOR PRODUCTION OF SOLUBLE SUGARS FROM CELLULOSIC BIOMASS
Granted: June 28, 2012
Application Number:
20120164709
The invention relates to recombinant expression of a variant form of a fungal C1 strain ?-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the ?-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant ?-glucosidase variant protein,…
GENE DISRUPTANTS PRODUCING FATTY ACYL-COA DERIVATIVES
Granted: June 28, 2012
Application Number:
20120165562
This invention provides microbial organisms, particularly yeasts such as Yarrowia lipolytica, that have one or more disrupted genes. The gene disruption(s) may yield improved production of fatty acyl-CoA derivatives.
RECOMBINANT BETA-GLUCOSIDASE VARIANTS FOR PRODUCTION OF SOLUBLE SUGARS FROM CELLULOSIC BIOMASS
Granted: June 28, 2012
Application Number:
20120164696
The invention relates to recombinant expression of a variant form of a fungal C1 strain ?-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the ?-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant ?-glucosidase variant protein,…
YEAST PROMOTERS
Granted: June 28, 2012
Application Number:
20120164686
The invention relates to recombinant promoters and expression constructs comprising the promoters that may be used to express a protein of interest in yeast, such as Yarrowia lipolytica.
VARIANT CBH2 CELLULASES AND RELATED POLYNUCLEOTIDES
Granted: June 21, 2012
Application Number:
20120156754
The invention provides variants of a Streptomyces sp. CBH2 that have improved properties compared to the wild type enzyme and methods of using the variants in the hydrolysis of substrates comprising cellulose.
Ketoreductase Polypeptides for the Preparation of Phenylephrine
Granted: June 14, 2012
Application Number:
20120149073
The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine.
NITRILASE BIOCATALYSTS
Granted: June 7, 2012
Application Number:
20120142063
The present disclosure relates to polypeptides having nitrilase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Stereomerically Pure Fused Bicyclic Proline Compounds Useful for Preparing Hepatitis C Protease Inhibitors
Granted: May 24, 2012
Application Number:
20120130087
The present disclosure provides substantially enantiomerically pure heterobicyclic compounds of the following structural formulas, wherein A, M, M?, and R5 are as described herein, and to biocatalytic processes for their preparation, and to the enzymes used in those processes.
FUNGAL STRAINS
Granted: May 3, 2012
Application Number:
20120107880
The present invention provides improved fungal strains. In some embodiments, the improved fungal strains find use in hydrolyzing cellulosic material to glucose.
COMPOSITIONS AND METHODS FOR PRODUCTION OF FERMENTABLE SUGARS
Granted: May 3, 2012
Application Number:
20120107881
The present application provides genetically modified fungal organisms that produce enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to glucose, enzyme mixtures produced by the genetically modified fungal organisms, and processes for producing glucose from cellulose using such enzyme mixtures.
Use of an Endogenous 2-Micron Yeast Plasmid for Gene Over Expression
Granted: April 12, 2012
Application Number:
20120088271
Methods and compositions for making stable recombinant yeast 2 ?m plasmids are provided. Homologous recombination is performed to clone a nucleic acid of interest into the yeast 2 ?m plasmid. Heterologous nucleic acid subsequences are recombined between an FLP and a REP2 gene of the plasmid.
Use of Glycoside Hydrolase 61 Family Proteins in Processing of Cellulose
Granted: April 5, 2012
Application Number:
20120083019
The invention provides recombinant GH61 proteins obtained from Myceliophtora thermophila, and nucleic acids that encode such proteins. The invention also provides protein fractions isolated from M. thermophila supernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, ?-glucosidase, or…
