Biocatalysts and methods for the synthesis of armodafinil
Granted: February 23, 2016
Patent Number:
9267159
The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (?)-2-[(R)-(diphenyl-methyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid,…
Cellobiohydrolase variants
Granted: February 16, 2016
Patent Number:
9260705
The present invention relates to cellobiohydrolase variants having improved thermostability in comparison to wild-type CBH2a.
Structure-activity relationships
Granted: January 5, 2016
Patent Number:
9228223
The present disclosure relates to compositions and methods for screening a plurality of polypeptide variants.
Synthesis of prazole compounds
Granted: January 5, 2016
Patent Number:
9228216
The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Polynucleotides encoding engineered ketoreductase polypeptides
Granted: January 5, 2016
Patent Number:
9228213
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the…
Engineered imine reductases and methods for the reductive animation of ketone and amine compounds
Granted: November 24, 2015
Patent Number:
9193957
The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
Variant LovD polypeptides and their uses
Granted: November 10, 2015
Patent Number:
9181570
The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound.
Recombinant C1 beta-glucosidase for production of sugars from cellulosic biomass
Granted: November 3, 2015
Patent Number:
9175324
The invention relates to expression of a recombinant C1 ?-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting cellobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 ?-glucosidase, operably linked to heterologous promoter, under conditions in which ?-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said ?-glucosidase to glucose. Methods of the…
Pentose fermentation by a recombinant microorganism
Granted: October 27, 2015
Patent Number:
9169468
The present invention provides methods and compositions suitable for use in the conversion of xylose to xylitol and xylulose, including nucleic acid constructs, recombinant fungal host cells, and related materials.
Fatty alcohol forming acyl reductases (FARs) and methods of use thereof
Granted: October 20, 2015
Patent Number:
9163221
The present disclosure provides methods useful for producing fatty alcohol compositions from recombinant host cells. The disclosure further provides variant fatty acyl-CoA reductase (FAR) enzymes, polynucleotides encoding the variant FAR enzymes, and vectors and host cells comprising the same.
Beta-glucosidase variants
Granted: October 6, 2015
Patent Number:
9150843
The invention relates to recombinantly produced ?-glucosidase variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant ?-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target ?-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.
Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs
Granted: September 22, 2015
Patent Number:
9139821
The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents. The present disclosure provides engineered polypeptides having transaminase activity, polynucleotides encoding the polypeptides, methods of the…
Ketoreductase polypeptides
Granted: September 22, 2015
Patent Number:
9139820
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered…
Biocatalysts for the preparation of hydroxy substituted carbamates
Granted: September 22, 2015
Patent Number:
9139819
The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
Transaminase biocatalysts
Granted: September 15, 2015
Patent Number:
9133445
The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Ketoreductase polypeptides for the production of azetidinone
Granted: September 15, 2015
Patent Number:
9133442
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Enone reductases
Granted: September 1, 2015
Patent Number:
9121045
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
Biocatalysts and methods for the synthesis of substituted lactams
Granted: August 18, 2015
Patent Number:
9109209
The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.
Variant CBH2 cellulases and related polynucleotides
Granted: August 11, 2015
Patent Number:
9102927
The invention provides variants of a Streptomyces sp. CBH2 that have improved properties compared to the wild type enzyme and methods of using the variants in the hydrolysis of substrates comprising cellulose.
Ketoreductase polypeptides for the preparation of phenylephrine
Granted: August 11, 2015
Patent Number:
9102959
The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine.
