Ketoreductase polypeptides for the production of ()-3-hydroxythiolane
Granted: July 19, 2016
Patent Number:
9394552
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.
Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system
Granted: July 19, 2016
Patent Number:
9394551
The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.
Enone reductases
Granted: July 12, 2016
Patent Number:
9388438
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs
Granted: July 12, 2016
Patent Number:
9388395
The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.
Biocatalysts for Ezetimibe synthesis
Granted: July 12, 2016
Patent Number:
9388391
The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Ketoreductase polypeptides for the production of azetidinone
Granted: July 5, 2016
Patent Number:
9382519
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Biocatalytic process for preparing eslicarbazepine and analogs thereof
Granted: June 14, 2016
Patent Number:
9365878
The present disclosure relates to biocatalysts and its uses for the efficient preparation of eslicarbazepine, eslicarbazepine acetate, and analogs thereof.
Biocatalysts and methods for the synthesis of armodafinil
Granted: June 14, 2016
Patent Number:
9365835
The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (?)-2-[(R)-(diphenylmethyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid,…
Transaminase biocatalysts
Granted: May 31, 2016
Patent Number:
9353355
The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
Granted: March 29, 2016
Patent Number:
9296993
The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
Ketoreductase-mediated stereoselective route to alpha chloroalcohols
Granted: March 29, 2016
Patent Number:
9296992
The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ?-chloroalcohols from ?-chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides.
Biocatalysts and methods for the synthesis of armodafinil
Granted: February 23, 2016
Patent Number:
9267159
The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (?)-2-[(R)-(diphenyl-methyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid,…
Cellobiohydrolase variants
Granted: February 16, 2016
Patent Number:
9260705
The present invention relates to cellobiohydrolase variants having improved thermostability in comparison to wild-type CBH2a.
Structure-activity relationships
Granted: January 5, 2016
Patent Number:
9228223
The present disclosure relates to compositions and methods for screening a plurality of polypeptide variants.
Synthesis of prazole compounds
Granted: January 5, 2016
Patent Number:
9228216
The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Polynucleotides encoding engineered ketoreductase polypeptides
Granted: January 5, 2016
Patent Number:
9228213
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the…
Engineered imine reductases and methods for the reductive animation of ketone and amine compounds
Granted: November 24, 2015
Patent Number:
9193957
The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
Variant LovD polypeptides and their uses
Granted: November 10, 2015
Patent Number:
9181570
The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound.
Recombinant C1 beta-glucosidase for production of sugars from cellulosic biomass
Granted: November 3, 2015
Patent Number:
9175324
The invention relates to expression of a recombinant C1 ?-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting cellobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 ?-glucosidase, operably linked to heterologous promoter, under conditions in which ?-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said ?-glucosidase to glucose. Methods of the…
Pentose fermentation by a recombinant microorganism
Granted: October 27, 2015
Patent Number:
9169468
The present invention provides methods and compositions suitable for use in the conversion of xylose to xylitol and xylulose, including nucleic acid constructs, recombinant fungal host cells, and related materials.
