Fatty alcohol forming acyl reductases (FARs) and methods of use thereof
Granted: October 20, 2015
Patent Number:
9163221
The present disclosure provides methods useful for producing fatty alcohol compositions from recombinant host cells. The disclosure further provides variant fatty acyl-CoA reductase (FAR) enzymes, polynucleotides encoding the variant FAR enzymes, and vectors and host cells comprising the same.
Beta-glucosidase variants
Granted: October 6, 2015
Patent Number:
9150843
The invention relates to recombinantly produced ?-glucosidase variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant ?-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target ?-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.
Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs
Granted: September 22, 2015
Patent Number:
9139821
The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents. The present disclosure provides engineered polypeptides having transaminase activity, polynucleotides encoding the polypeptides, methods of the…
Ketoreductase polypeptides
Granted: September 22, 2015
Patent Number:
9139820
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered…
Biocatalysts for the preparation of hydroxy substituted carbamates
Granted: September 22, 2015
Patent Number:
9139819
The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
Transaminase biocatalysts
Granted: September 15, 2015
Patent Number:
9133445
The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Ketoreductase polypeptides for the production of azetidinone
Granted: September 15, 2015
Patent Number:
9133442
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Enone reductases
Granted: September 1, 2015
Patent Number:
9121045
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
Biocatalysts and methods for the synthesis of substituted lactams
Granted: August 18, 2015
Patent Number:
9109209
The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.
Biocatalytic process for preparing eslicarbazepine and analogs thereof
Granted: August 11, 2015
Patent Number:
9102963
The present disclosure relates to biocatalysts and its uses for the efficient preparation of eslicarbazepine, eslicarbazepine acetate, and analogs thereof.
Ketoreductase polypeptides for the preparation of phenylephrine
Granted: August 11, 2015
Patent Number:
9102959
The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine.
Variant CBH2 cellulases and related polynucleotides
Granted: August 11, 2015
Patent Number:
9102927
The invention provides variants of a Streptomyces sp. CBH2 that have improved properties compared to the wild type enzyme and methods of using the variants in the hydrolysis of substrates comprising cellulose.
Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system
Granted: July 14, 2015
Patent Number:
9080192
The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.
Cellobiohydrolase variants
Granted: July 14, 2015
Patent Number:
9080163
The invention relates to recombinant expression of variant forms of C1 CBH1a and homologs thereof, having improved thermostability, low-pH tolerance, specific activity and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.
Fungal strains
Granted: June 30, 2015
Patent Number:
9068235
The present invention provides improved fungal strains. In some embodiments, the improved fungal strains find use in hydrolyzing cellulosic material to glucose.
Lov-D acyltransferase mediated acylation
Granted: June 16, 2015
Patent Number:
9057085
Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.
Pentose fermentation by a recombinant microorganism
Granted: June 2, 2015
Patent Number:
9045745
The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.
Biocatalysts for ezetimibe synthesis
Granted: May 26, 2015
Patent Number:
9040262
The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Transaminase polypeptides
Granted: May 12, 2015
Patent Number:
9029106
The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.
Ketoreductase-mediated stereoselective route to alpha chloroalcohols
Granted: May 12, 2015
Patent Number:
9029112
The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ?-chloroalcohols from ?-chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides.
