Variant endoglucanases and related polynucleotides
Granted: April 14, 2015
Patent Number:
9005946
The invention provides variants of the Clostridium thermocellum endoglucanase (CelG) that have improved endoglucanase activity compared to the wild type enzyme. Also provided are related polynucleotides, compositions, vectors, host cells, and methods of use.
Polynucleotides encoding beta-glucosidase variants
Granted: March 31, 2015
Patent Number:
8993277
The present invention provides compositions and methods for the expression of recombinant ?-glucosidase variants, as well as their use in the production of fermentable sugars from cellulosic biomass.
Production of monoterpenes
Granted: March 24, 2015
Patent Number:
8986965
The present invention relates to methods for producing monoterpenes, particularly tricyclene, by culturing microbial organisms that express a terpene synthase and optionally a prenyl transferase.
Ketoreductase polypeptides for the production of azetidinone
Granted: March 17, 2015
Patent Number:
8980606
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Ketoreductases and uses thereof
Granted: March 17, 2015
Patent Number:
8980605
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Fungal beta-xylosidase variants
Granted: March 17, 2015
Patent Number:
8980578
The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using…
Ketoreductase polypeptides for the production of (R)-3-hydroxythiolane
Granted: February 24, 2015
Patent Number:
8962285
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.
Engineered ketoreductase polypeptides
Granted: February 17, 2015
Patent Number:
8956840
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered…
?-glucosidase variants
Granted: February 17, 2015
Patent Number:
8956845
The invention relates to recombinantly produced ?-glucosidase variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant ?-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target ?-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.
Fungal xylanases and xylosidases
Granted: February 17, 2015
Patent Number:
8956844
The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by…
GH61 glycoside hydrolase protein variants and cofactors that enhance GH61 activity
Granted: February 10, 2015
Patent Number:
8951758
The present invention provides various GH61 protein variants comprising various amino acid substitutions. The GH61 protein variants have an improved ability to synergize with cellulase enzymes, thereby increasing the yield of fermentable sugars obtained by saccharification of biomass. In some embodiments, sugars obtained from saccharification are fermented to produce numerous end-products, including but not limited to alcohol.
Cellobiohydrolase variants
Granted: February 3, 2015
Patent Number:
8945903
The present invention relates to cellobiohydrolase variants having improved thermostability in comparison to wild-type CBH2a.
Biocatalysts and methods for the synthesis of ()-3-(1-aminoethyl)-phenol
Granted: January 13, 2015
Patent Number:
8932838
The present disclosure provides engineered transaminase polypeptides having improved properties as compared to naturally occurring transaminases including the ability of converting the substrate, 3?-hydroxyacetophenone to (S)-3-(1-aminoethyl)-phenol in enantiomeric excess and high percentage conversion. Also provided are polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases…
Biocatalysts and methods for the synthesis of (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine
Granted: January 13, 2015
Patent Number:
8932836
The disclosure provides transaminase polypeptides capable of converting the substrate, 2-(3,4-dimethoxyphenethoxy)cyclohexanone to the trans diastereomer product (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine in at least a 2:1 diastereomeric ratio relative to the cis diastereomer (1R,2S)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine. The disclosure also provides polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides in processes for preparing…
Transaminase reactions
Granted: December 30, 2014
Patent Number:
8921079
The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.
Multi-cellulase enzyme compositions for hydrolysis of cellulosic biomass
Granted: December 23, 2014
Patent Number:
8916366
The invention relates to a multi-cellulase enzyme composition for the enzymatic hydrolysis of cellulosic biomass said composition comprising a cellobiohydrolase (CBH) enzyme, an endoglucanase (EG) enzyme and a ?-glucosidase (BG) enzyme.
Endoglucanase variants
Granted: December 9, 2014
Patent Number:
8906689
The present invention relates to variant endoglucanases having improved thermoactivity, improved thermostability, and improved viscosity reduction activity over wild-type M. thermophila endoglucanase.
Polynucleotides encoding recombinant C1 beta-glucosidase for production of sugars from cellulosic biomass
Granted: December 2, 2014
Patent Number:
8900847
The invention relates to expression of a recombinant C1 ?-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting celiobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 ?-glucosidase, operably linked to heterologous promoter, under conditions in which ?-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said ?-glucosidase to glucose. Methods of the…
Synthesis of prazole compounds
Granted: November 25, 2014
Patent Number:
8895271
The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Transaminase biocatalysts
Granted: November 18, 2014
Patent Number:
8889380
The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
