Cellobiohydrolase variants
Granted: February 3, 2015
Patent Number:
8945903
The present invention relates to cellobiohydrolase variants having improved thermostability in comparison to wild-type CBH2a.
Biocatalysts and methods for the synthesis of ()-3-(1-aminoethyl)-phenol
Granted: January 13, 2015
Patent Number:
8932838
The present disclosure provides engineered transaminase polypeptides having improved properties as compared to naturally occurring transaminases including the ability of converting the substrate, 3?-hydroxyacetophenone to (S)-3-(1-aminoethyl)-phenol in enantiomeric excess and high percentage conversion. Also provided are polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases…
Biocatalysts and methods for the synthesis of (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine
Granted: January 13, 2015
Patent Number:
8932836
The disclosure provides transaminase polypeptides capable of converting the substrate, 2-(3,4-dimethoxyphenethoxy)cyclohexanone to the trans diastereomer product (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine in at least a 2:1 diastereomeric ratio relative to the cis diastereomer (1R,2S)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine. The disclosure also provides polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides in processes for preparing…
Transaminase reactions
Granted: December 30, 2014
Patent Number:
8921079
The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.
Multi-cellulase enzyme compositions for hydrolysis of cellulosic biomass
Granted: December 23, 2014
Patent Number:
8916366
The invention relates to a multi-cellulase enzyme composition for the enzymatic hydrolysis of cellulosic biomass said composition comprising a cellobiohydrolase (CBH) enzyme, an endoglucanase (EG) enzyme and a ?-glucosidase (BG) enzyme.
Endoglucanase variants
Granted: December 9, 2014
Patent Number:
8906689
The present invention relates to variant endoglucanases having improved thermoactivity, improved thermostability, and improved viscosity reduction activity over wild-type M. thermophila endoglucanase.
Polynucleotides encoding recombinant C1 beta-glucosidase for production of sugars from cellulosic biomass
Granted: December 2, 2014
Patent Number:
8900847
The invention relates to expression of a recombinant C1 ?-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting celiobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 ?-glucosidase, operably linked to heterologous promoter, under conditions in which ?-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said ?-glucosidase to glucose. Methods of the…
Synthesis of prazole compounds
Granted: November 25, 2014
Patent Number:
8895271
The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Transaminase biocatalysts
Granted: November 18, 2014
Patent Number:
8889380
The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
Enone reductases
Granted: November 11, 2014
Patent Number:
8883475
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
Production of fatty alcohols with fatty alcohol forming acyl-CoA reductases (FAR)
Granted: November 11, 2014
Patent Number:
8883467
The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
Polynucleotides encoding engineered ketoreductase polypeptides
Granted: November 4, 2014
Patent Number:
8877475
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the…
GH61 glycoside hydrolase protein variants and cofactors that enhance GH61 activity
Granted: November 4, 2014
Patent Number:
8877474
The present invention provides various GH61 protein variants comprising various amino acid substitutions. The GH61 protein variants have an improved ability to synergize with cellulase enzymes, thereby increasing the yield of fermentable sugars obtained by saccharification of biomass. In some embodiments, sugars obtained from saccharification are fermented to produce numerous end-products, including but not limited to alcohol.
Stereomerically pure fused bicyclic proline compounds useful for preparing hepatitis C protease inhibitors
Granted: October 14, 2014
Patent Number:
8859784
The present disclosure provides substantially enantiomerically pure heterobicyclic compounds of the following structural formulas, wherein A, M, M?, and R5 are as described herein, and to biocatalytic processes for their preparation, and to the enzymes used in those processes.
Ketoreductase polypeptides for the reduction of acetophenones
Granted: October 7, 2014
Patent Number:
8852909
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Biocatalysts and methods for the synthesis of ()-3-(1-aminoethyl)-phenol
Granted: October 7, 2014
Patent Number:
8852900
The present disclosure provides engineered transaminase polypeptides having improved properties as compared to naturally occurring transaminases including the ability of converting the substrate, 3?-hydroxyacetophenone to (S)-3-(1-aminoethyl)-phenol in enantiomeric excess and high percentage conversion. Also provided are polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases…
Methods, systems, and software for identifying functional biomolecules
Granted: September 30, 2014
Patent Number:
8849575
The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention…
Variant LOVD polypeptides and their uses
Granted: September 30, 2014
Patent Number:
8846362
The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound.
Polypeptides for a ketoreductase-mediated stereoselective route to alpha chloroalcohols
Granted: August 5, 2014
Patent Number:
8796002
The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ? chloroalcohols from ? chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides.
Variant CBH2 cellulases and related polynucleotides
Granted: July 22, 2014
Patent Number:
8785170
The invention provides variants of a Streptomyces sp. CBH2 that have improved properties compared to the wild type enzyme and methods of using the variants in the hydrolysis of substrates comprising cellulose.
